Construct: ORF TRCN0000489903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021790.1_s317c1
- DNA Barcode:
- TCAGATCACACCCTGTTTGCAGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- VRK2 (7444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489903
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130480.2 | 100% | 100% | |
| 2 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130481.2 | 100% | 100% | |
| 3 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288837.1 | 100% | 100% | |
| 4 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_006296.6 | 100% | 100% | |
| 5 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_005264540.4 | 100% | 100% | |
| 6 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_011533092.3 | 100% | 100% | |
| 7 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130482.2 | 95.4% | 95.4% | 0_1ins69 |
| 8 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712090.4 | 77.7% | 77.5% | 1184_1186delTAGinsA;1191_1191delTins336 |
| 9 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004858.2 | 77.7% | 77.5% | 1184_1186delTAGinsA;1191_1191delTins336 |
| 10 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130483.2 | 77.6% | 77.5% | 1183_1185delTTTinsGAA;1188_1188delGins337 |
| 11 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288838.1 | 77.6% | 77.5% | 1183_1185delTTTinsGAA;1188_1188delGins337 |
| 12 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712091.4 | 77.6% | 77.5% | 1183_1185delTTTinsGAA;1188_1188delGins337 |
| 13 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288836.1 | 76.7% | 76.7% | 0_1ins354 |
| 14 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288839.1 | 76.7% | 76.7% | 0_1ins354 |
| 15 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712092.4 | 76.7% | 76.7% | 0_1ins354 |
| 16 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712093.4 | 76.7% | 76.7% | 0_1ins354 |
| 17 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004859.2 | 76.7% | 76.7% | 0_1ins354 |
| 18 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004860.2 | 76.7% | 76.7% | 0_1ins354 |
| 19 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004861.2 | 76.7% | 76.7% | 0_1ins354 |
| 20 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004862.2 | 76.7% | 76.7% | 0_1ins354 |
| 21 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004863.2 | 76.7% | 76.7% | 0_1ins354 |
| 22 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004865.1 | 54.4% | 54.3% | 0_1ins354;829_831delTTTinsGAA;834_834delGins337 |
| 23 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004866.1 | 54.4% | 54.3% | 0_1ins354;829_831delTTTinsGAA;834_834delGins337 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1593
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccaccaaaa agaaatgaaa aatacaaact tcctattcca tttccagaag 121 gcaaggttct ggatgatatg gaaggcaatc agtgggtact gggcaagaag attggctctg 181 gaggatttgg attgatatat ttagctttcc ccacaaataa accagagaaa gatgcaagac 241 atgtagtaaa agtggaatat caagaaaatg gcccgttatt ttcagaactt aaattttatc 301 agagagttgc aaaaaaagac tgtatcaaaa agtggataga acgcaaacaa cttgattatt 361 taggaattcc tctgttttat ggatctggtc tgactgaatt caagggaaga agttacagat 421 ttatggtaat ggaaagacta ggaatagatt tacagaagat ctcaggccag aatggtacct 481 ttaaaaagtc aactgtcctg caattaggta tccgaatgtt ggatgtactg gaatatatac 541 atgaaaatga atatgttcat ggtgatataa aagcagcaaa tctacttttg ggttacaaaa 601 atccagacca ggtttatctt gcagattatg gactttccta cagatattgt cccaatggga 661 accacaaaca gtatcaggaa aatcctagaa aaggccataa tgggacaata gagtttacca 721 gcttggatgc ccacaaggga gtagccttgt ccagacgaag tgacgttgag atcctcggct 781 actgcatgct gcggtggttg tgtgggaaac ttccctggga acagaacctg aaggaccctg 841 tggctgtgca gactgctaaa acaaatctgt tggacgagct cccccagtca gtgcttaaat 901 gggctccttc tggaagcagt tgctgtgaaa tagcccaatt tttggtatgt gctcatagtt 961 tagcatatga tgaaaagcca aactatcaag ccctcaagaa aattttgaac cctcatggaa 1021 tacctttagg accactggac ttttccacaa aaggacagag tataaatgtc catactccaa 1081 acagtcaaaa agttgattca caaaaggctg caacaaagca agtcaacaag gcacacaata 1141 ggttaatcga aaaaaaagtc cacagtgaga gaagcgctga gtcctgtgca acatggaaag 1201 tgcagaaaga ggagaaactg attggattga tgaaCAATGA AGCAGCTCAG GAAAGCACAA 1261 GGAGAAGACA GAAATATCAA GAGTCTCAAG AACCTTTGAA TGAAGTAAAC AGTTTCCCAC 1321 AAAAAATCAG CTATACACAA TTCCCAAACT CATTTTATGA GCCTCATCAA GATTTTACCA 1381 GTCCAGATAT ATTCAAGAAG TCAAGATCTC CATCTTGGTA TAAATACACT TCCACAGTCA 1441 GCACGGGGAT CACAGACTTA GAAAGTTCAA CTGGACTTTG GCCTACAATT TCCCAGTTTA 1501 CTCTTAGTGA AGAGACAAAC GCAGATGTTT ATTATTATCG CATCATCATA CCTGTCCTTT 1561 TGATGTTAGT ATTTCTTGCT TTATTTTTTC TCTGAGACCC AGCTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 ATCAGATCAC ACCCTGTTTG CAGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt