Construct: ORF TRCN0000489906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020720.1_s317c1
- DNA Barcode:
- ATAATGCAGGTGGGCCGATGTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK33 (65975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489906
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289061.2 | 100% | 100% | |
| 2 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352387.2 | 100% | 100% | |
| 3 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352388.2 | 100% | 100% | |
| 4 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352389.2 | 100% | 100% | |
| 5 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352390.2 | 100% | 100% | |
| 6 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352391.2 | 100% | 100% | |
| 7 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352392.2 | 100% | 100% | |
| 8 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352393.2 | 100% | 100% | |
| 9 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_030906.4 | 100% | 100% | |
| 10 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520290.2 | 100% | 100% | |
| 11 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520295.2 | 100% | 100% | |
| 12 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018144.2 | 100% | 100% | |
| 13 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018145.1 | 100% | 100% | |
| 14 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018146.1 | 100% | 100% | |
| 15 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018147.1 | 100% | 100% | |
| 16 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018148.1 | 100% | 100% | |
| 17 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018152.1 | 100% | 100% | |
| 18 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018153.2 | 100% | 100% | |
| 19 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018154.1 | 100% | 100% | |
| 20 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018155.2 | 100% | 100% | |
| 21 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018156.1 | 100% | 100% | |
| 22 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289058.2 | 92% | 92% | 0_1ins123 |
| 23 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352394.2 | 92% | 92% | 0_1ins123 |
| 24 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352395.2 | 92% | 92% | 0_1ins123 |
| 25 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520297.2 | 92% | 92% | 0_1ins123 |
| 26 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018157.1 | 92% | 92% | 0_1ins123 |
| 27 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352399.2 | 87.1% | 87.1% | 1146_1147ins198 |
| 28 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018159.1 | 87.1% | 87.1% | 1146_1147ins198 |
| 29 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018161.1 | 79.1% | 72.5% | (many diffs) |
| 30 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352396.2 | 71.1% | 64.7% | (many diffs) |
| 31 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352397.2 | 71.1% | 64.7% | (many diffs) |
| 32 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352398.2 | 71.1% | 64.7% | (many diffs) |
| 33 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289059.2 | 63.6% | 63.6% | 0_1ins561 |
| 34 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747938.1 | 34.7% | (many diffs) | |
| 35 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747939.1 | 26% | 1_2568del;3439_3440ins76;3638_3639ins396 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 1641
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccgtcg acaaacaaat ggctgatagt ggcttagata 121 aaaaatccac aaaatgcccc gactgttcat ctgcttctca gaaagatgta ctttgtgtat 181 gttccagcaa aacaagggtt cctccagttt tggtggtgga aatgtcacag acatcaagca 241 ttggtagtgc agaatcttta atttcactgg agagaaaaaa agaaaaaaat atcaacagag 301 atataacctc caggaaagat ttgccctcaa gaacctcaaa tgtagagaga aaagcatctc 361 agcaacaatg gggtcggggc aactttacag aaggaaaagt tcctcacata aggattgaga 421 atggagctgc tattgaggaa atctatacct ttggaagaat attgggaaaa gggagctttg 481 gaatagtcat tgaagcgaca gacaaggaaa cagaaacgaa gtgggcaatt aaaaaagtga 541 acaaagaaaa ggctggaagc tctgctgtga agttacttga acgagaggtg aacattctga 601 aaagtgtaaa acatgaacac atcatacatc tggaacaagt atttgaaacg ccaaagaaaa 661 tgtaccttgt gatggagctt tgtgaggatg gagaactcaa agaaattctg gataggaaag 721 ggcatttctc agagaatgag acaaggtgga tcattcaaag tctcgcatca gctatagcat 781 atcttcacaa taatgatatt gtacatagag atctgaaact ggaaaatata atggttaaaa 841 gcagtcttat tgatgataac aatgaaataa acttaaacat aaaggtgact gattttggct 901 tagcggtgaa gaagcaaagt aggagtgaag ccatgctgca ggccacatgt gggactccta 961 tctatatggc ccctgaagtt atcagtgccc acgactatag ccagcagtgt gacatttgga 1021 gcataggcgt cgtaatgtac atgttattac gtggagaacc accctttttg gcaagctcag 1081 aagagaagct ttttgagtta ataagaaaag gagaactaca ttttgaaaat gcagtctgga 1141 attccataag tgactgtgct aaaagtgttt tgaaacaact tatgaaagta gatcctgctc 1201 acagaatcac agctaaggaa ctactagata accagtggtt aacaggcaat aaactttcTT 1261 CGGTGAGACC AACCAATGTA TTAGAGATGA TGAAGGAATG GAAAAATAAC CCAGAAAGTG 1321 TTGAGGAAAA CACAACAGAA GAGAAGAATA AGCCGTCCAC TGAAGAAAAG TTGAAAAGTT 1381 ACCAACCCTG GGGAAATGTC CCTGATGCCA ATTACACTTC AGATGAAGAG GAGGAAAAAC 1441 AGTCTACTGC TTATGAAAAG CAATTTCCTG CAACCAGTAA GGACAACTTT GATATGTGCA 1501 GTTCAAGTTT CACATCTAGC AAACTCCTTC CAGCTGAAAT CAAGGGAGAA ATGGAGAAAA 1561 CCCCTGTGAC TCCAAGCCAA GGAACAGCAA CCAAGTACCC TGCTAAATCC GGCGCCCTGT 1621 CCAGAACCAA AAAGAAACTC TAAGGATCCA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA ATAATGCAGG TGGGCCGATG TCAAACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt