Construct: ORF TRCN0000489920
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021649.1_s317c1
- DNA Barcode:
- TATGCTACCACAGAGAAAGCAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BCL2L1 (598)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489920
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001317919.2 | 100% | 100% | |
| 2 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001317920.2 | 100% | 100% | |
| 3 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001317921.2 | 100% | 100% | |
| 4 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001322239.2 | 100% | 100% | |
| 5 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001322240.2 | 100% | 100% | |
| 6 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001322242.2 | 100% | 100% | |
| 7 | human | 598 | BCL2L1 | BCL2 like 1 | NM_138578.3 | 100% | 100% | |
| 8 | human | 598 | BCL2L1 | BCL2 like 1 | XM_011528964.2 | 100% | 100% | |
| 9 | human | 598 | BCL2L1 | BCL2 like 1 | XM_017027993.1 | 100% | 100% | |
| 10 | human | 598 | BCL2L1 | BCL2 like 1 | NM_001191.4 | 72.9% | 72.9% | 374_375ins189 |
| 11 | human | 598 | BCL2L1 | BCL2 like 1 | NR_134257.1 | 28.2% | 1_112del;674_830del;969_2474del | |
| 12 | human | 598 | BCL2L1 | BCL2 like 1 | XR_001754364.2 | 25.7% | 1_363del;925_1081del;1220_2713del | |
| 13 | human | 598 | BCL2L1 | BCL2 like 1 | XR_936599.3 | 25.5% | 1_381del;943_1099del;1238_2731del | |
| 14 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | NM_001289716.1 | 93.5% | 97.8% | (many diffs) |
| 15 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | NM_001289717.1 | 93.5% | 97.8% | (many diffs) |
| 16 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | NM_009743.5 | 93.5% | 97.8% | (many diffs) |
| 17 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | XM_006498609.3 | 93.5% | 97.8% | (many diffs) |
| 18 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | XM_006498611.3 | 93.5% | 97.8% | (many diffs) |
| 19 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | XM_006498612.2 | 93.5% | 97.8% | (many diffs) |
| 20 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | XM_011239260.2 | 93.5% | 97.8% | (many diffs) |
| 21 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | NM_001289739.1 | 80.3% | 71.6% | (many diffs) |
| 22 | mouse | 12048 | Bcl2l1 | BCL2-like 1 | XR_374396.3 | 15% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 768
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtctcagagc aaccgggagc tggtggttga ctttctctcc tacaagcttt 121 cccagaaagg atacagctgg agtcagttta gtgatgtgga agagaacagg actgaggccc 181 cagaagggac tgaatcggag atggagaccc ccagtgccat caatggcaac ccatcctggc 241 acctggcaga cagccccgcg gtgaatggag ccaCTGGCCA CAGCAGCAGT TTGGATGCCC 301 GGGAGGTGAT CCCCATGGCA GCAGTAAAGC AAGCGCTGAG GGAGGCAGGC GACGAGTTTG 361 AACTGCGGTA CCGGCGGGCA TTCAGTGACC TGACATCCCA GCTCCACATC ACCCCAGGGA 421 CAGCATATCA GAGCTTTGAA CAGGTAGTGA ATGAACTCTT CCGGGATGGG GTAAACTGGG 481 GTCGCATTGT GGCCTTTTTC TCCTTCGGCG GGGCACTGTG CGTGGAAAGC GTAGACAAGG 541 AGATGCAGGT ATTGGTGAGT CGGATCGCAG CTTGGATGGC CACTTACCTG AATGACCACC 601 TAGAGCCTTG GATCCAGGAG AACGGCGGCT GGGATACTTT TGTGGAACTC TATGGGAACA 661 ATGCAGCAGC CGAGAGCCGA AAGGGCCAGG AACGCTTCAA CCGCTGGTTC CTGACGGGCA 721 TGACTGTGGC CGGCGTGGTT CTGCTGGGCT CACTCTTCAG TCGGAAATAG AACCCAGCTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 901 GACGATATGC TACCACAGAG AAAGCAATCA CGCGTTAAGT Cgacaatcaa cctctggatt 961 acaaaatttg tgaaagatt