Construct: ORF TRCN0000489935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020781.1_s317c1
- DNA Barcode:
- GCTCGGGTGGTGGACCCCCTTGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LRFN5 (145581)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145581 | LRFN5 | leucine rich repeat and fib... | NM_001346173.1 | 99.9% | 100% | 1284A>G;1566T>C |
| 2 | human | 145581 | LRFN5 | leucine rich repeat and fib... | NM_152447.4 | 99.9% | 100% | 1284A>G;1566T>C |
| 3 | human | 145581 | LRFN5 | leucine rich repeat and fib... | XM_017021048.1 | 99.9% | 100% | 1284A>G;1566T>C |
| 4 | human | 145581 | LRFN5 | leucine rich repeat and fib... | NM_001330106.1 | 64.6% | 64.2% | (many diffs) |
| 5 | human | 145581 | LRFN5 | leucine rich repeat and fib... | NM_001346175.1 | 64.6% | 64.2% | (many diffs) |
| 6 | human | 145581 | LRFN5 | leucine rich repeat and fib... | NR_144389.1 | 46.4% | (many diffs) | |
| 7 | human | 145581 | LRFN5 | leucine rich repeat and fib... | NR_144388.1 | 45.1% | (many diffs) | |
| 8 | mouse | 238205 | Lrfn5 | leucine rich repeat and fib... | NM_001310586.1 | 91.3% | 96.8% | (many diffs) |
| 9 | mouse | 238205 | Lrfn5 | leucine rich repeat and fib... | XM_017315056.1 | 91.3% | 96.8% | (many diffs) |
| 10 | mouse | 238205 | Lrfn5 | leucine rich repeat and fib... | NM_178714.5 | 87.3% | 91.4% | (many diffs) |
| 11 | mouse | 238205 | Lrfn5 | leucine rich repeat and fib... | XM_006515830.2 | 87.3% | 91.4% | (many diffs) |
| 12 | mouse | 238205 | Lrfn5 | leucine rich repeat and fib... | XM_006515831.3 | 87.3% | 91.4% | (many diffs) |
| 13 | mouse | 238205 | Lrfn5 | leucine rich repeat and fib... | XR_001780450.1 | 40.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2229
- ORF length:
- 2157
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaaaa attctttttt atctgtttct cattggcata gcagtgaaag 121 ctcagatctg tccaaagcgt tgtgtctgtc agattttgtc tcctaatctt gcaacccttt 181 gtgccaagaa agggctttta tttgttccac caaacattga cagaagaact gtggaactgc 241 ggttggcaga caattttgtt acaaatatta aaaggaaaga ttttgccaat atgaccagct 301 tggtggacct gactctatcc aggaatacaa taagttttat tacacctcat gctttcgctg 361 acctacgaaa tttgagggct ttgcatttga atagcaacag attgactaaa attacaaatg 421 atatgttcag tggtctttcc aatcttcatc atttgatact gaacaacaat cagctgactt 481 taatttcctc tacagcgttt gatgatgtct tcgcccttga ggagctggat ctgtcctata 541 ataatctaga aaccattcct tgggatgctg ttgagaagat ggttagcttg cataccctta 601 gtttggatca caatatgatt gataacattc ctaaggggac cttctcccat ttgcacaaga 661 tgactcggtt agatgtgaca tcaaataaat tgcagaagct accacctgac cctctctttc 721 agcgagctca ggtactagca acctcaggaa tcataagccc atctactttt gcattaagtt 781 ttggtggaaa ccccttgcat tgcaattgtg aattgttgtg gttgaggcgt ctgtccagag 841 aagatgactt agagacctgt gcttctcctc cacttttaac tggccgctac ttttggtcaa 901 ttcctgaaga agagtttttg tgtgagcctc ctctcattac tcgtcataca catgagatga 961 gagtcctgga gggacaaagg gcaacactga ggtgcaaagc caggggagac cctgagcctg 1021 caattcactg gatttctcct gaagggaagc ttatttcaaa tgcaacaaga tctctggtgt 1081 atgataacgg aacacttgac attcttatca caactgtaaa ggatacaggt gcttttacct 1141 gcattgcttc caatcctgct ggggaagcaa cacaaatagt ggatcttcat ataattaagc 1201 tccctcactt actaaatagt acaaaccata tccatgagcc tgatcctggt tcttcagata 1261 tctcaacttc taccaagtca ggttctaata caagcagtag taatggtgat actaaattga 1321 gtcaagataa aattgtggtg gcagaagcta catcgtcaac ggcactactt aaatttaatt 1381 ttcaaagaaa tatccctgga atacgtatgt ttcaaatcca gtacaatggt acttatgatg 1441 acacccttgt ttacagaatg atacctccta cgagcaaaac ttttctggtc aataatctgg 1501 ctgctggaac tatgtatgac ttgtgtgtct tggccatata tgatgatggc atcacttccc 1561 tcactgccac aagagtcgtg ggttgcatcc agtttactac ggaacaggat tatgtgcgtt 1621 gccatttcat gcagtcccag tttttgggag gcaccatgat tattattatt ggtggaatca 1681 ttgtagcatc tgtgctggta ttcatcatta ttcTGATGAT CCGGTATAAG GTTTGCAACA 1741 ATAATGGGCA ACACAAGGTC ACCAAGGTTA GCAATGTTTA TTCCCAAACT AACGGGGCTC 1801 AAATACAAGG CTGTAGTGTA ACGCTGCCCC AGTCCGTGTC CAAACAAGCT GTGGGACACG 1861 AAGAGAATGC CCAGTGTTGT AAAGCTACCA GTGACAATGT GATTCAATCT TCAGAAACTT 1921 GTTCGAGTCA GGACTCCTCT ACCACTACCT CTGCTTTGCC TCCTTCCTGG ACTTCAAGCA 1981 CTTCTGTGTC CCAAAAGCAG AAAAGAAAGA CTGGCACAAA GCCAAGTACA GAACCACAGA 2041 ATGAAGCCGT CACAAATGTT GAATCCCAAA ACACTAACAG GAACAACTCA ACTGCCTTGC 2101 AGTTAGCTAG CCGTCCTCCC GATTCTGTCA CAGAGGGGCC CACGTCTAAA AGAGCACATA 2161 TAAAGCCAAA TGCTTTGCTG ACTAATGTTG ACCAGATTGT CCAGGAAACA CAGAGGCTGG 2221 AGTTAATCTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2281 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2341 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCT CGGGTGGTGG ACCCCCTTGG 2401 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t