Construct: ORF TRCN0000489937
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020299.1_s317c1
- DNA Barcode:
- GGCACATGTTGTATCCCCAGCACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ITK (3702)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489937
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3702 | ITK | IL2 inducible T cell kinase | NM_005546.4 | 99.7% | 99.8% | 1098G>C;1860_1861insTTG |
| 2 | human | 3702 | ITK | IL2 inducible T cell kinase | XM_017009443.1 | 79.6% | 79.7% | 0_1ins375;723G>C;1485_1486insTTG |
| 3 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_010583.3 | 87.7% | 94% | (many diffs) |
| 4 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281968.1 | 87.6% | 93.8% | (many diffs) |
| 5 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281966.1 | 87.3% | 93.5% | (many diffs) |
| 6 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281965.1 | 86.9% | 93.1% | (many diffs) |
| 7 | mouse | 16428 | Itk | IL2 inducible T cell kinase | NM_001281967.1 | 37.4% | 38.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1935
- ORF length:
- 1863
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacaac tttatcctcc tggaagaaca gctcatcaag aaatcccaac 121 aaaagagaag aacttctccc tcgaacttta aagtccgctt ctttgtgtta accaaagcca 181 gcctggcata ctttgaagat cgtcatggga agaagcgcac gctgaagggg tccattgagc 241 tctcccgaat caaatgtgtt gagattgtga aaagtgacat cagcatccca tgccactata 301 aatacccgtt tcaggtggtg catgacaact acctcctata tgtgtttgct ccagatcgtg 361 agagccggca gcgctgggtg ctggccctta aagaagaaac gaggaataat aacagtttgg 421 tgcctaaata tcatcctaat ttctggatgg atgggaagtg gaggtgctgt tctcagctgg 481 agaagcttgc aacaggctgt gcccaatatg atccaaccaa gaatgcttca aagaagcctc 541 ttcctcctac tcctgaagac aacaggcgac cactttggga acctgaagaa actgtggtca 601 ttgccttata tgactaccaa accaatgatc ctcaggaact cgcactgcgg cgcaacgaag 661 agtactgcct gctggacagt tctgagattc actggtggag agtccaggac aggaatgggc 721 atgaaggata tgtaccaagc agttatctgg tggaaaaatc tccaaataat ctggaaacct 781 atgagtggta caataagagt atcagccgag acaaagctga aaaacttctt ttggacacag 841 gcaaagaagg agccttcatg gtaagggatt ccaggactgc aggaacatac accgtgtctg 901 ttttcaccaa ggctgttgta agtgagaaca atccctgtat aaagcattat cacatcaagg 961 aaacaaatga caatcctaag cgatactatg tggctgaaaa gtatgtgttc gattccatcc 1021 ctcttctcat caactatcac caacataatg gaggaggcct ggtgactcga ctccggtatc 1081 cagtttgttt tgggaggcag aaagccccag ttacagcagg gctgagatac gggaaatggg 1141 tgatcgaccc ctcagagctc acttttgtcc aagagattgg cagtgggcaa tttgggttgg 1201 tgcatctggg ctactggctc aacaaggaca aggtggctat caaaaccatt cgggaagggg 1261 ctatgtcaga agaggacttc atagaggagg ctgaagtaat gatgaaactc tctcatccca 1321 aactggtgca gctgtatggg gtgtgcctgg agcaggcccc catctgcctg gtgtttgagt 1381 tcatggagca cggctgcctg tcagattatc tacgcaccca gcggggactt tttgctgcag 1441 agaccctgct gggcatgtgt ctggatgtgt gtgagggcat ggcctacctg gaagaggcat 1501 gtgTCATCCA CAGAGACTTG GCTGCCAGAA ATTGTTTGGT GGGAGAAAAC CAAGTCATCA 1561 AGGTGTCTGA CTTTGGGATG ACAAGGTTCG TTCTGGATGA TCAGTACACC AGTTCCACAG 1621 GCACCAAATT CCCGGTGAAG TGGGCATCCC CAGAGGTTTT CTCTTTCAGT CGCTATAGCA 1681 GCAAGTCCGA TGTGTGGTCA TTTGGTGTGC TGATGTGGGA AGTTTTCAGT GAAGGCAAAA 1741 TCCCGTATGA AAACCGAAGC AACTCAGAGG TGGTGGAAGA CATCAGTACC GGATTTCGGT 1801 TGTACAAGCC CCGGCTGGCC TCCACACACG TCTACCAGAT TATGAATCAC TGCTGGAAAG 1861 AGAGACCAGA AGATCGGCCA GCCTTCTCCA GACTGCTGCG TCAACTGGCT GAAATTGCAG 1921 AATCAGGACT TTTGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1981 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2041 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCACAT GTTGTATCCC 2101 CAGCACTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt