Construct: ORF TRCN0000489951
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020694.1_s317c1
- DNA Barcode:
- TCAACAACCCATGATATTGCCACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK32A (202374)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489951
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 202374 | STK32A | serine/threonine kinase 32A | NM_145001.4 | 99.7% | 100% | 9G>C |
| 2 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_011537579.2 | 72.5% | 68.1% | (many diffs) |
| 3 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_017009213.1 | 52.3% | 49.5% | (many diffs) |
| 4 | human | 202374 | STK32A | serine/threonine kinase 32A | NM_001112724.2 | 41.2% | 39.2% | (many diffs) |
| 5 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_011537577.2 | 40.9% | 38.9% | (many diffs) |
| 6 | human | 202374 | STK32A | serine/threonine kinase 32A | NM_001287740.2 | 40.1% | 38.1% | (many diffs) |
| 7 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_011537576.1 | 37.6% | 34.5% | (many diffs) |
| 8 | human | 202374 | STK32A | serine/threonine kinase 32A | XR_001742038.1 | 14.9% | (many diffs) | |
| 9 | human | 202374 | STK32A | serine/threonine kinase 32A | XR_001742037.1 | 6.8% | (many diffs) | |
| 10 | mouse | 269019 | Stk32a | serine/threonine kinase 32A | NM_178749.3 | 38.5% | 37.2% | (many diffs) |
| 11 | mouse | 64293 | Stk32b | serine/threonine kinase 32B | NM_022416.2 | 32.4% | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 570
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggagcc aaCACTTCAA GAAAACCACC AGTGTTTGAT GAAAATGAAG 121 ATGTCAACTT TGACCACTTT GAAATTTTGC GAGCCATTGG GAAAGGCAGT TTTGGGAAGG 181 TCTGCATTGT ACAGAAGAAT GATACCAAGA AGATGTACGC AATGAAGTAC ATGAATAAAC 241 AAAAGTGCGT GGAGCGCAAT GAAGTGAGAA ATGTCTTCAA GGAACTCCAG ATCATGCAGG 301 GTCTGGAGCA CCCTTTCCTG GTTAATTTGT GGTATTCCTT CCAAGATGAG GAAGACATGT 361 TCATGGTGGT GGACCTCCTG CTGGGTGGAG ACCTGCGTTA TCACCTGCAA CAGAACGTCC 421 ACTTCAAGGA AGAAACAGTG AAGCTCTTCA TCTGTGAGCT GGTCATGGCC CTGGACTACC 481 TGCAGAACCA GCGCATCATT CACAGGGATA TGAAGCCTGA CAATATTTTA CTTGACGAAC 541 ATGATACCTG GCTCTCCTAC AAGTCCCACT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 721 AACAACCCAT GATATTGCCA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt