Construct: ORF TRCN0000489953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019142.1_s317c1
- DNA Barcode:
- TAAGCATATTTTGAGATTGTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RYK (6259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6259 | RYK | receptor like tyrosine kinase | NM_001005861.2 | 99.9% | 99.8% | 1830_1831insG |
| 2 | human | 6259 | RYK | receptor like tyrosine kinase | NM_002958.3 | 99.4% | 99.3% | 889_890insGCTCCTTAG;1821_1822insG |
| 3 | human | 6259 | RYK | receptor like tyrosine kinase | XR_924160.2 | 55.9% | 1_180del;1356_1357ins133;1878_2900delinsG | |
| 4 | mouse | 20187 | Ryk | receptor-like tyrosine kinase | NM_013649.3 | 87.9% | 92.6% | (many diffs) |
| 5 | mouse | 20187 | Ryk | receptor-like tyrosine kinase | NM_001042607.1 | 87.5% | 92.3% | (many diffs) |
| 6 | mouse | 20187 | Ryk | receptor-like tyrosine kinase | NM_001284258.1 | 70.7% | 76.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1905
- ORF length:
- 1833
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgtggg gcggcgcggc tggggcggcc gggccggagt tgcctcccgg 121 gggcccgcgg cctgagggcc ccgccgccgc cgccgctgct gcttctgctt gcgctgttgc 181 cgctgctgcc cgcgcctggc gctgccgccg cccccgcccc gcggcccccg gagctgcagt 241 cggcttccgc ggggcccagc gtgagtctct acctgagcga ggacgaggtg cgccggctga 301 tcggtcttga tgcagaactt tattatgtga gaaatgacct tattagtcac tacgctctat 361 cctttagtct gttagtaccc agtgagacaa atttcctgca cttcacctgg catgcgaagt 421 ccaaggttga atataagctg ggattccaag tggacaatgt tttggcaatg gatatgcccc 481 aggtcaacat ttctgttcag ggggaagttc cacgcacttt atcagtgttt cgggtagagc 541 tttcctgtac tggcaaagta gattctgaag ttatgatact aatgcagctc aacttgacag 601 taaattcttc aaaaaatttt accgtcttaa attttaaacg aaggaaaatg tgctacaaaa 661 aacttgaaga agtaaaaact tcagccttgg acaaaaacac tagcagaact atttatgatc 721 ctgtacatgc agctccaacc acttctacgc gtgtgtttta tattagtgta ggggtttgtt 781 gtgcagtaat atttctcgta gcaataatat tagctgtttt gcaccttcat agtatgaaaa 841 ggattgaact ggatgacagc attagtgcca gcagtagttc ccaagggctg tctcagccat 901 ccacccagac gactcagtat ctgagagcag acacgcccaa caatgcaact cctatcacca 961 gctccttagg ttatcctacc ttgcggatag agaagaacga cttgagaagt gtcactcttt 1021 tggaggccaa aggcaaggtg aaggatatag caatatccag agagaggata actctaaaag 1081 atgtactcca agaaggtact tttgggcgta ttttccatgg gattttaata gatgaaaaag 1141 atccaaataa agaaaaacaa gcatttgtca aaacagttaa agatcaagct tctgaaattc 1201 aggtgacaat gatgctcact gaaagttgta agctgcgagg tcttcatcac agaaatcttc 1261 ttcctattac tcatgtgtgt atagaagaag gagaaaagcc catggtgata ttgccttaca 1321 tgaattgggg gaatcttaaa ttgtttttac gacagtgcaa gttagtagag gccaataatc 1381 cacaggcaat ttctcagcaa gacctggtac acatggctat tcagattgcc tgtggaatga 1441 gctacctggc cagaagggaa gtcatccaca aagacctggc tgccaggaac tgtGTCATTG 1501 ATGACACACT TCAAGTTAAG ATCACAGACA ATGCCCTCTC CAGAGACTTG TTCCCCATGG 1561 ACTATCACTG TCTGGGGGAC AATGAAAACA GGCCAGTTCG TTGGATGGCT CTTGAAAGTC 1621 TGGTTAATAA CGAGTTCTCT AGCGCTAGTG ATGTGTGGGC CTTTGGAGTG ACGCTGTGGG 1681 AACTCATGAC TCTGGGCCAG ACTCCCTACG TGGACATTGA CCCCTTCGAG ATGGCCGCAT 1741 ACCTGAAAGA TGGTTACCGA ATAGCCCAGC CAATCAACTG TCCTGATGAA TTATTTGCTG 1801 TGATGGCCTG TTGCTGGGCC TTAGATCCAG AGGAGAGGCC CAAGTTTCAG CAGCTGGTAC 1861 AGTGCCTAAC AGAGTTTCAT GCAGCCCTGG GGGCCTACGT CGACCCAGCT TTCTTGTACA 1921 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1981 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2041 AGGACGATAA GCATATTTTG AGATTGTTAT TACGCGTTAA GTCgacaatc aacctctgga 2101 ttacaaaatt tgtgaaagat t