Transcript: Human NM_002958.3

Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
RYK (6259)
Length:
2933
CDS:
91..1914

Additional Resources:

NCBI RefSeq record:
NM_002958.3
NBCI Gene record:
RYK (6259)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149332 TGAACAGAAATGTTGACCTG pXPR_003 GGG 411 23% 3 0.9199 RYK RYK 76450
2 BRDN0001146690 ACTGAAAGTTGTAAGCTGCG pXPR_003 AGG 1154 63% 10 0.1158 RYK RYK 76452
3 BRDN0001146411 CGTGAGTCTCTACCTGAGCG pXPR_003 AGG 205 11% 1 0.0178 RYK RYK 76451
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002958.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023719 CCACGCACTTTATCAGTGTTT pLKO.1 529 CDS 100% 4.950 6.930 N Ryk n/a
2 TRCN0000278116 CCACGCACTTTATCAGTGTTT pLKO_005 529 CDS 100% 4.950 6.930 N Ryk n/a
3 TRCN0000196324 GCCTTAGAAATGCTTTAGAAT pLKO.1 2037 3UTR 100% 5.625 4.500 N RYK n/a
4 TRCN0000380054 AGCTCAACTTGACAGTAAATT pLKO_005 605 CDS 100% 15.000 10.500 N RYK n/a
5 TRCN0000338273 TGCGAAGTCCAAGGTTGAATA pLKO_005 432 CDS 100% 13.200 9.240 N RYK n/a
6 TRCN0000338220 TGTGAGAAATGACCTTATTAG pLKO_005 345 CDS 100% 13.200 9.240 N RYK n/a
7 TRCN0000338329 ATTTGGAATTAGCTATCTTAG pLKO_005 2240 3UTR 100% 10.800 7.560 N RYK n/a
8 TRCN0000381622 CAACTCCTATCACCAGTTATC pLKO_005 965 CDS 100% 10.800 7.560 N RYK n/a
9 TRCN0000196474 GATGACACACTTCAAGTTAAG pLKO.1 1510 CDS 100% 10.800 7.560 N RYK n/a
10 TRCN0000338219 GATGACACACTTCAAGTTAAG pLKO_005 1510 CDS 100% 10.800 7.560 N RYK n/a
11 TRCN0000382518 GATGTAAGTGACTCACCTTTA pLKO_005 2297 3UTR 100% 10.800 7.560 N RYK n/a
12 TRCN0000001573 GCAAGTTAGTAGAGGCCAATA pLKO.1 1367 CDS 100% 10.800 7.560 N RYK n/a
13 TRCN0000338272 GCAAGTTAGTAGAGGCCAATA pLKO_005 1367 CDS 100% 10.800 7.560 N RYK n/a
14 TRCN0000196255 GTCTTCAAGATGCAATACTTT pLKO.1 2364 3UTR 100% 5.625 3.938 N RYK n/a
15 TRCN0000001572 CAACGCCAACAGAAGCACATT pLKO.1 1998 3UTR 100% 4.950 3.465 N RYK n/a
16 TRCN0000001574 GCCCAACAATGCAACTCCTAT pLKO.1 954 CDS 100% 4.950 3.465 N RYK n/a
17 TRCN0000195387 CGGATAGAGAAGAACGACTTG pLKO.1 994 CDS 100% 4.050 2.835 N RYK n/a
18 TRCN0000023721 GCGGATAGAGAAGAACGACTT pLKO.1 993 CDS 100% 4.050 2.835 N Ryk n/a
19 TRCN0000278182 GCGGATAGAGAAGAACGACTT pLKO_005 993 CDS 100% 4.050 2.835 N Ryk n/a
20 TRCN0000001576 CAAGTTAAGATCACAGACAAT pLKO.1 1522 CDS 100% 4.950 2.970 N RYK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002958.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489927 GTCACAAGTAGCACTAAGCGGGGT pLX_317 23.5% 99.5% 99.5% V5 (not translated due to prior stop codon) 889_890insGCTCCTTAG n/a
2 TRCN0000489953 TAAGCATATTTTGAGATTGTTATT pLX_317 22.5% 99.4% 99.3% V5 889_890insGCTCCTTAG;1821_1822insG n/a
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