Construct: ORF TRCN0000489962
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019487.1_s317c1
- DNA Barcode:
- CGCTTCAACGAGGCTGAGACCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT1 (207)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489962
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_001014431.2 | 97.2% | 96.9% | (many diffs) |
| 2 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_001014432.1 | 97.2% | 96.9% | (many diffs) |
| 3 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_005163.2 | 97.2% | 96.9% | (many diffs) |
| 4 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | XR_002957536.1 | 33.6% | (many diffs) | |
| 5 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | NM_001331107.1 | 88.3% | 95.3% | (many diffs) |
| 6 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | NM_009652.3 | 88.3% | 95.3% | (many diffs) |
| 7 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | XM_006515415.1 | 88.3% | 95.3% | (many diffs) |
| 8 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | NM_001165894.1 | 84.5% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 1557
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccatg ggtagcaaca agagcaagcc caaggatgcc agccagcgga 121 gcgacgtggc tattgtgaag gagggttggc tgcacaaacg aggggagtac atcaagacct 181 ggcggccacg ctacttcctc ctcaagaatg atggcacctt cattggctac aaggagcggc 241 cgcaggatgt ggaccaacgt gaggctcccc tcaacaactt ctctgtggcg cagtgccagc 301 tgatgaagac ggagcggccc cggcccaaca ccttcatcat ccgctgcctg cagtggacca 361 ctgtcatcga acgcaccttc catgtggaga ctcctgagga gcgggaggag tggacaaccg 421 ccatccagac tgtggctgac ggcctcaaga agcaggagga ggaggagatg gacttccggt 481 cgggctcacc cagtgacaac tcaggggctg aagagatgga ggtgtccctg gccaagccca 541 agcaccgcgt gaccatgaac gagtttgagt acctgaagct gctgggcaag ggcactttcg 601 gcaaggtgat cctggtgaag gagaaggcca caggccgcta ctacgccatg aagatcctca 661 agaaggaagt catcgtggcc aaggacgagg tggcccacac actcaccgag aaccgcgtct 721 tgcagaactc caggcacccc ttcctcacag ccctgaagta ctctttccag acccacgacc 781 gcctctgctt tgtcatggag tacgccaacg ggggcgagct gttcttccac ctgtcccggg 841 agcgtgtgtt ctccgaggac cgggcccgct tctatggcgc tgagattgtg tcagccctgg 901 actacctgca ctcggagaag aacgtggtgt accgggacct caagctggag aacctcatgc 961 tggacaagga cgggcacatt aagatcacag acttcgggct gtgcaaggag gggatcaagg 1021 acggtgccac catgaagacc ttttgcggca cacctgagta cctggccccc gaggtgctgg 1081 aggacaatga ctacggccgt gcagtggact ggtgggggct gggcgtggtc atgtacgaga 1141 tgatgtgcgg tcgcctgccc ttctacaacc aggaccatga gaagcttttt gagctcatcc 1201 tcatGGAGGA GATCCGCTTC CCGCGCACGC TTGGTCCCGA GGCCAAGTCC TTGCTTTCAG 1261 GGCTGCTCAA GAAGGACCCC AAGCAGAGGC TTGGCGGGGG CTCCGAGGAC GCCAAGGAGA 1321 TCATGCAGCA TCGCTTCTTT GCCGGTATCG TGTGGCAGCA CGTGTACGAG AAGAAGCTCA 1381 GCCCACCCTT CAAGCCCCAG GTCACGTCGG AGACTGACAC CAGGTATTTT GATGAGGAGT 1441 TCACGGCCCA GATGATCACC ATCACACCAC CTGACCAAGA TGACAGCATG GAGTGTGTGG 1501 ACAGCGAGCG CAGGCCCCAC TTCCCCCAGT TCTCCTACTC GGCCAGCAGC ACGGCCAAGG 1561 GTGGGCGCGC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1621 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1681 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGC TTCAACGAGG CTGAGACCTT 1741 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t