Construct: ORF TRCN0000491257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015669.1_s317c1
- Derived from:
- ccsbBroadEn_06794
- DNA Barcode:
- TTGAAGTGCGGCCAAAAACCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSKH1 (5681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5681 | PSKH1 | protein serine kinase H1 | NM_006742.3 | 99.9% | 99.7% | 17G>T |
| 2 | mouse | 244631 | Pskh1 | protein serine kinase H1 | NM_173432.2 | 88.4% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1338
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgtgggaca atcaaggtcc ttcccgagcc acccaaggat gtccagctgg 121 atctggtcaa gaaggtggag cccttcagtg gcactaagag tgacgtgtac aagcacttca 181 tcacagaggt ggacagtgtt ggccctgtca aagccgggtt cccagcagca agtcagtatg 241 cacacccctg ccccggtccc ccgactgctg gccacacgga gcctccctca gaaccaccac 301 gcagggccag ggtagctaag tacagggcca agtttgaccc acgtgttaca gctaagtatg 361 acatcaaggc cctaattggc cgaggcagct tcagccgagt ggtacgtgta gagcaccggg 421 caacccggca gccgtatgcc atcaagatga ttgagaccaa gtaccgggag gggcgggagg 481 tgtgtgagtc ggagctgcgt gtgctgcgtc gggtgcgtca tgccaacatc atccagctgg 541 tggaggtgtt cgagacacag gagcgggtgt acatggtgat ggagctggcc actggtggag 601 agctctttga ccgcatcatt gccaagggct ccttcaccga gcgtgacgcc acgcgggtgc 661 tgcagatggt gctggatggc gtccggtatc tgcatgcact gggcatcaca caccgagacc 721 tcaaacctga gaatctgctc tactaccatc cgggcactga ctccaagatc atcatcaccg 781 acttcggcct ggccagtgct cgcaagaagg gtgatgactg cttgatgaag accacctgtg 841 gcacgcctga gtacattgcc ccagaagtcc tggtccgcaa gccatacacc aactcagtgg 901 acatgtgggc gctgggcgtc attgcctaca tcctactcag tggcaccatg ccgtttgagg 961 atgacaaccg tacccggctg taccggcaga tcctcagggg caagtacagt tactctgggg 1021 agccctggcc tagtgtgtcc aacctggcca aggacttcat tgaccgcctg ctgacagtgg 1081 accctggagc ccgtatgact gcactgcagg ccctgaggca cccgtgggtg gtgagcatgg 1141 ctgcctcttc atccatgaag aacctgcacc gctccatatc ccagaacctc cTTAAACGTG 1201 CCTCCTCGCG CTGCCAGAGC ACCAAATCTG CCCAGTCCAC GCGTTCCAGC CGCTCCACAC 1261 GCTCCAATAA GTCACGCCGT GTGCGGGAAC GGGAGCTGCG GGAGCTCAAC CTGCGCTACC 1321 AGCAGCAATA CAATGGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1381 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1441 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGAAGT GCGGCCAAAA 1501 ACCTACGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt