Construct: ORF TRCN0000491261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021325.2_s317c1
- DNA Barcode:
- CGGAAAGATACCACACAATGCCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ISG20 (3669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303237.2 | 52% | 45.2% | (many diffs) |
| 2 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_017022148.1 | 52% | 45.2% | (many diffs) |
| 3 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303233.2 | 44.9% | 42.5% | (many diffs) |
| 4 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303234.2 | 44.9% | 42.5% | (many diffs) |
| 5 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_002201.6 | 44.9% | 42.5% | (many diffs) |
| 6 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_005254899.2 | 44.9% | 42.5% | (many diffs) |
| 7 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_006720488.3 | 44.9% | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 333
- ORF length:
- 261
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctggg agccgtgagg tggtggccat ggactgcgag atggtggggc 121 tggggcccca ccgggagagt gGCCTGGCTC GTTGCAGCCT CGTGAACGTC CACGGTGCTG 181 TGCTGTACGA CAAGTTCATC CGGCCTGAGG GAGAGATCAC CGATTACAGA ACCCGGGTCA 241 GCGGGGTCAC CCCTCAGCAC ATGGTGGGGG CCACACCATT TGCCGTGGCC AGGCTAGAGG 301 TCCCCTTCCC ATCCTCTCCA ACGGCAGCTG TGTAGAACCC AGCTTTCTTG TACAAAGTGG 361 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 421 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 481 ACGGAAAGAT ACCACACAAT GCCTAACGCG TTAAGTCgac aatcaacctc tggattacaa 541 aatttgtgaa agatt