Construct: ORF TRCN0000491269
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020785.2_s317c1
- DNA Barcode:
- AAATCCCGCTTGTTCCGGCGGATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADRB2 (154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491269
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154 | ADRB2 | adrenoceptor beta 2 | NM_000024.5 | 99.9% | 99.7% | 647T>C |
| 2 | mouse | 11555 | Adrb2 | adrenergic receptor, beta 2 | NM_007420.3 | 84.5% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1311
- ORF length:
- 1239
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggcaa cccgggaacg gcagcgcctt cttgctggca cccaatagaa 121 gccatgcgcc ggaccacgac gtcacgcagc aaagggacga ggtgtgggtg gtgggcatgg 181 gcatcgtcat gtctctcatc gtcctggcca tcgtgtttgg caatgtgctg gtcatcacag 241 ccattgccaa gttcgagcgt ctgcagacgg tcaccaacta cttcatcact tcactggcct 301 gtgctgatct ggtcatgggc ctggcagtgg tgccctttgg ggccgcccat attcttatga 361 aaatgtggac ttttggcaac ttctggtgcg agttttggac ttccattgat gtgctgtgcg 421 tcacggccag cattgagacc ctgtgcgtga tcgcagtgga tcgctacttt gccattactt 481 cacctttcaa gtaccagagc ctgctgacca agaataaggc ccgggtgatc attctgatgg 541 tgtggattgt gtcaggcctt acctccttct tgcccattca gatgcactgg taccgggcca 601 cccaccagga agccatcaac tgctatgcca atgagacctg ctgtgacttc ttcacgaacc 661 aagcctatgc cattgcctct tccatcgtgt ccttctacgt tcccctggtg atcatggcct 721 tcgtctactc cagggtcttt caggaggcca aaaggcagct ccagaagatt gacaaatctg 781 agggccgctt ccatgtccag aaccttagcc aggtggagca ggatgggcgg acggggcatg 841 gactccgcag atcttccaag ttctgcttga aggagcacaa agccctcaag acgttaggca 901 tcatcatggg cactttcacc ctctgctggc tgcccttctt catcgttaac attgtgcatg 961 tgatccagga taacctcatc cgtaaggaag tttacatcct cctaaattGG ATAGGCTATG 1021 TCAATTCTGG TTTCAATCCC CTTATCTACT GCCGGAGCCC AGATTTCAGG ATTGCCTTCC 1081 AGGAGCTTCT GTGCCTGCGC AGGTCTTCTT TGAAGGCCTA TGGGAATGGC TACTCCAGCA 1141 ACGGCAACAC AGGGGAGCAG AGTGGATATC ACGTGGAACA GGAGAAAGAA AATAAACTGC 1201 TGTGTGAAGA CCTCCCAGGC ACGGAAGACT TTGTGGGCCA TCAAGGTACT GTGCCTAGCG 1261 ATAACATTGA TTCACAAGGG AGGAATTGTA GTACAAATGA CTCACTGCTG TAAGACCCAG 1321 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1381 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1441 TATCTTGTGG AAAGGACGAA AATCCCGCTT GTTCCGGCGG ATAACGCGTT AAGTCgacaa 1501 tcaacctctg gattacaaaa tttgtgaaag att