Construct: ORF TRCN0000491281

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021270.2_s317c1
DNA Barcode:
ACTAATCGTCTACCCTGAGCTCTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ARL17A (51326)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491281
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51326 ARL17A ADP ribosylation factor lik... NM_001288813.1 85.4% 84.4% 260_261insTAAAGATTA;264_265ins36
2 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_006725288.3 85.4% 84.4% 260_261insTAAAGATTA;264_265ins36
3 human 100506084 ARL17B ADP ribosylation factor lik... NM_001330240.1 85.2% 84.6% (many diffs)
4 human 51326 ARL17A ADP ribosylation factor lik... NM_001330244.1 85.2% 84.6% (many diffs)
5 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_011546391.2 85.2% 84.6% (many diffs)
6 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_017030181.2 85.1% 83.4% 191G>C;260_261insTAAAGATTA;264_265ins36
7 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_011546379.2 84.9% 83.6% (many diffs)
8 human 100506084 ARL17B ADP ribosylation factor lik... NM_001103154.2 76.5% 72.8% (many diffs)
9 human 51326 ARL17A ADP ribosylation factor lik... NM_001288812.1 76.5% 72.8% (many diffs)
10 human 100506084 ARL17B ADP ribosylation factor lik... NM_001352769.1 76.5% 72.8% (many diffs)
11 human 51326 ARL17A ADP ribosylation factor lik... NM_016632.2 76.5% 72.8% (many diffs)
12 human 51326 ARL17A ADP ribosylation factor lik... XM_005257439.5 76.5% 72.8% (many diffs)
13 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_006725284.3 76.5% 72.8% (many diffs)
14 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_024452506.1 76.5% 72.8% (many diffs)
15 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_024452507.1 76.5% 72.8% (many diffs)
16 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_011546390.3 67.5% 58.5% (many diffs)
17 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_011546376.3 67.2% 57.8% (many diffs)
18 human 51326 ARL17A ADP ribosylation factor lik... NM_001288811.1 64.9% 62.7% (many diffs)
19 human 100506084 ARL17B ADP ribosylation factor lik... NM_001039083.4 52.9% 51.1% (many diffs)
20 human 51326 ARL17A ADP ribosylation factor lik... NM_001113738.1 52.9% 51.1% (many diffs)
21 human 100506084 ARL17B ADP ribosylation factor lik... NM_001363805.1 51.7% 50% (many diffs)
22 human 51326 ARL17A ADP ribosylation factor lik... XM_011524877.3 51.7% 50% (many diffs)
23 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_011546389.2 51.7% 50% (many diffs)
24 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_011546369.3 51.6% 49.4% (many diffs)
25 human 51326 ARL17A ADP ribosylation factor lik... XM_011524876.3 47.9% 46.1% (many diffs)
26 human 51326 ARL17A ADP ribosylation factor lik... XM_017024729.2 47.9% 46.1% (many diffs)
27 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_017030176.1 47.9% 46.1% (many diffs)
28 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_017030180.2 47.7% 45.6% (many diffs)
29 human 51326 ARL17A ADP ribosylation factor lik... XM_011524875.3 46.3% 44.6% (many diffs)
30 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XM_011546387.2 46.3% 44.6% (many diffs)
31 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_011546367.3 46.1% 44.1% (many diffs)
32 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XM_017030179.2 43.4% 41.4% (many diffs)
33 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_002958882.1 34.1% (many diffs)
34 human 51326 ARL17A ADP ribosylation factor lik... XR_934477.3 30.5% (many diffs)
35 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_951555.2 29.6% (many diffs)
36 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756214.1 29.5% (many diffs)
37 human 51326 ARL17A ADP ribosylation factor lik... XR_934479.2 27.7% (many diffs)
38 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756211.2 27.7% (many diffs)
39 human 51326 ARL17A ADP ribosylation factor lik... XR_934475.3 25.8% (many diffs)
40 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_951553.3 25.1% (many diffs)
41 human 51326 ARL17A ADP ribosylation factor lik... XR_934476.3 24.8% (many diffs)
42 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_951558.2 23.3% (many diffs)
43 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756217.1 23.2% (many diffs)
44 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756218.2 21.7% (many diffs)
45 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_951557.2 19.4% (many diffs)
46 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756215.1 19.3% (many diffs)
47 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_002958881.1 18.5% (many diffs)
48 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_001756189.1 18.1% (many diffs)
49 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_951554.2 17.9% (many diffs)
50 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756213.2 17.8% (many diffs)
51 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756210.2 17.1% (many diffs)
52 human 100996709 LOC100996709 ADP-ribosylation factor-lik... XR_951556.2 15.1% (many diffs)
53 human 51326 ARL17A ADP ribosylation factor lik... XR_934478.3 14.2% (many diffs)
54 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_002958883.1 12.3% (many diffs)
55 human 51326 ARL17A ADP ribosylation factor lik... XR_934480.3 11% (many diffs)
56 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756216.2 10.9% (many diffs)
57 human 107984156 LOC107984156 ADP-ribosylation factor-lik... XR_001756212.2 10.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
381
ORF length:
309
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgggaaac atttttgaaa agctctttaa aagtctactt gggaaaaaaa
121agatgcggat tcttatattg agtttGGATA CAGCTGGAAA AACCACCATC TTGTATAAAT
181TGAAGCTGGG GGAGACTGTG CCTGCCGTCC CTACAGTAGG TTTCTGTGTG GAGACAGTAG
241AATATAAAAA TAACACCTTC GCTGTCTGGG ATGTTGGCAG CCACTTCAAA ATCAGACCTC
301TGTGGCAGCA TTTTTTCCAG AACACAAAAG GTAAAGATTA TTCGGTTTGT AGCCTCGATA
361CTGTTTCTGT GAAATGTCTC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
421AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
481TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTAATCGTCT
541ACCCTGAGCT CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
601att

Download FASTA (ORF) (Full)