Transcript: Human XM_011546367.3

PREDICTED: Homo sapiens ADP-ribosylation factor-like protein 17 (LOC107984156), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LOC107984156 (107984156)
Length:
1280
CDS:
219..830

Additional Resources:

NCBI RefSeq record:
XM_011546367.3
NBCI Gene record:
LOC107984156 (107984156)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011546367.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000344420 GTGTGGAGACAGTAGAATATA pLKO_005 373 CDS 100% 15.000 7.500 Y ARL17B n/a
2 TRCN0000344481 AGCAGCGGTTTCCCAAGTTTC pLKO_005 690 CDS 100% 10.800 5.400 Y ARL17B n/a
3 TRCN0000353088 CAGACCTCTGTGGCAGCATTT pLKO_005 440 CDS 100% 10.800 5.400 Y ARL17B n/a
4 TRCN0000263038 CGGATTCTTATATTGAGTTTG pLKO_005 273 CDS 100% 10.800 5.400 Y ARL17B n/a
5 TRCN0000140294 GATGTTGGCAGCCACTTCAAA pLKO.1 417 CDS 100% 5.625 2.813 Y ARL17A n/a
6 TRCN0000371106 GAATGTGGAAAGGAGGTAGAA pLKO_005 559 CDS 100% 4.950 2.475 Y ARL17B n/a
7 TRCN0000141827 GAGTTTGGATACAGCTGGAAA pLKO.1 287 CDS 100% 4.950 2.475 Y ARL17A n/a
8 TRCN0000140598 GCCAGGTAACACCAAAGCTTT pLKO.1 1017 3UTR 100% 4.950 2.475 Y ARL17A n/a
9 TRCN0000371107 TGCCGTCCCTACAGTAGGTTT pLKO_005 350 CDS 100% 4.950 2.475 Y ARL17B n/a
10 TRCN0000263039 TGGTCAGCTGGACTCCATACT pLKO_005 623 CDS 100% 4.950 2.475 Y ARL17B n/a
11 TRCN0000140324 CAGTAGGTTTCTGTGTGGAGA pLKO.1 361 CDS 100% 2.640 1.320 Y ARL17A n/a
12 TRCN0000139147 CCCATCTCTTGGACAGATGAT pLKO.1 843 3UTR 100% 0.495 0.248 Y ARL17A n/a
13 TRCN0000166030 CCTCTGGAAATCAGCTGTGTT pLKO.1 725 CDS 100% 4.950 2.475 Y NBR2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011546367.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11975 pDONR223 100% 77.4% 77.3% None (many diffs) n/a
2 ccsbBroad304_11975 pLX_304 0% 77.4% 77.3% V5 (many diffs) n/a
3 TRCN0000472181 ATTATGTTATCGCGATTGCCACTT pLX_317 93.6% 77.4% 77.3% V5 (many diffs) n/a
4 TRCN0000491281 ACTAATCGTCTACCCTGAGCTCTT pLX_317 73.6% 46.1% 44.1% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_14147 pDONR223 100% 35.2% 30% None (many diffs) n/a
6 ccsbBroad304_14147 pLX_304 0% 35.2% 30% V5 (many diffs) n/a
7 TRCN0000472660 ACATCAACTGCAGGATTCTATGTG pLX_317 100% 35.2% 30% V5 (many diffs) n/a
8 ccsbBroadEn_11473 pDONR223 100% 29.5% 28.5% None (many diffs) n/a
9 ccsbBroad304_11473 pLX_304 0% 29.5% 28.5% V5 (many diffs) n/a
10 TRCN0000479820 CGTGGTACGCCAGCATATGCCAGA pLX_317 100% 29.5% 28.5% V5 (many diffs) n/a
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