Construct: ORF TRCN0000491285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007044.2_s317c1
- Derived from:
- ccsbBroadEn_04618
- DNA Barcode:
- GAGCAAATTCGCCGCCAGCTTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYTL5 (94122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94122 | SYTL5 | synaptotagmin like 5 | NM_001163334.1 | 100% | 100% | |
| 2 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_011544001.2 | 100% | 100% | |
| 3 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_011544002.2 | 100% | 100% | |
| 4 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_017029972.1 | 100% | 100% | |
| 5 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_017029973.1 | 100% | 100% | |
| 6 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_017029974.1 | 100% | 100% | |
| 7 | human | 94122 | SYTL5 | synaptotagmin like 5 | NM_001163335.2 | 97% | 97% | 1152_1153ins66 |
| 8 | human | 94122 | SYTL5 | synaptotagmin like 5 | NM_138780.3 | 97% | 97% | 1152_1153ins66 |
| 9 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | NM_177704.3 | 85.9% | 84.2% | (many diffs) |
| 10 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_006527604.3 | 85.9% | 84.2% | (many diffs) |
| 11 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_006527607.3 | 85.9% | 84.2% | (many diffs) |
| 12 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_006527608.3 | 85.9% | 84.2% | (many diffs) |
| 13 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247460.2 | 85.9% | 84.2% | (many diffs) |
| 14 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247463.2 | 85.9% | 84.2% | (many diffs) |
| 15 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247464.1 | 85.9% | 84.2% | (many diffs) |
| 16 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247465.1 | 85.9% | 84.2% | (many diffs) |
| 17 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247468.1 | 85.9% | 84.2% | (many diffs) |
| 18 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247469.2 | 85.9% | 84.2% | (many diffs) |
| 19 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247470.2 | 85.9% | 84.2% | (many diffs) |
| 20 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247471.2 | 85.9% | 84.2% | (many diffs) |
| 21 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_017318467.1 | 85.9% | 84.2% | (many diffs) |
| 22 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247472.1 | 85.6% | 83.9% | (many diffs) |
| 23 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | NM_001290728.1 | 83.4% | 81.6% | (many diffs) |
| 24 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247473.1 | 83.4% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2325
- ORF length:
- 2256
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctaagaac tcagagttca tcaatctgtc atttttatta gatcatgaga 121 aggaaatgat cctgggcgtc ctaaagagag atgaatattt gaaaaaagtg gaggacaaga 181 gaataaggaa gctgaaaaat gaactcttag aagcaaaacg tagaagtggg aaaactcaac 241 aagaggccag cagagtttgt gttcactgtc acagaaacct gggcctaatc tttgaccggg 301 gagacccttg tcaggcttgc tcactgaggg tatgcaggga gtgtcgagtt gcaggcccca 361 atggcagctg gaagtgcact gtctgtgaca aaatcgcgca gctaaggatt ataactggtg 421 agtggttttt tgaagaaaag gcaaaacgtt tcaagcaagt caatgttctc ggcactgatg 481 ttgtccgaca gtccatttta agaagaagtc caggagctga agaagtacag agccaagagc 541 aaacccgcca ggatgcagaa aagtcagaca cttcacctgt tgctgggaag aaggccagcc 601 atgatgggcc caagagaaag ggatttcttc ttagcaagtt cagatcggca accagaggag 661 aaatcataac tcccaaaact gacactgggc ggagctatag cttggactta gacggtcaac 721 attttcggag tttaaaatca cctcctggtt cagacagggg aagcactggc tcatcagatc 781 tcaatgacca ggaacctggt cctaggaccc cgaagagcag tcggagcaat ggtgtgaccc 841 caggcactca gagttcacca gccccaagca cacgaactgt gacctcagtc atcagtagag 901 agtatggttt tgaaaattcc atggatttgg ctgctattga aggtacctct caggagctca 961 caaagagtca ccgcagaaac acttctggca caccttccat agcagtgtct ggaacctctc 1021 tctcctcaga tcagagtcga tctgagttag atttgagtga gtcatttaca gaagactcag 1081 aggatactgt aagcataaga agcaagtctg tccctggggc tttagacaag gactccttgg 1141 aagagactga agaaagcatt gatgccttag tgtcctcgca gttatctaca aacactcacc 1201 gtctggcaag tggcctatca actagttctc aagcaggttc tgacaggaag tggacctacc 1261 taaatgtgcc tgatgctgac tcagacacta ccagccttaa cagcatgatg agcgtttaca 1321 gtgaaacggg agactatggc aacgtgaaag tcagtggtga aatccttctc catatcagct 1381 actgctacaa aactggtggg ctgtacattt ttgtcaagaa ttgcagaaat ctggccatag 1441 gagatgaaaa gaaacagagg acagatgctt atgtcaagtc atatcttctt cctgacaagt 1501 cccggaacaa caagcgtaag accaaaatca gaacaggcac caatccagaa ttcaatgaaa 1561 cactaaagta cactatcagc catacccagc tggaaacaag aactctgcag ctctcagtct 1621 ggcactatga tcgatttgga cgtaatagct tcctcgggga agtagagatt ccttttgact 1681 catggaactt tgaaaatcca actgatgagt ggtttgtgct tcaacccaag gtggagtttg 1741 ctcctgatat tggccttcaa tacaaaggag agctgacagt tgttttacgt tacattcccc 1801 cagaagagaa cctgatgctt ccaccagaac aactccaagg aaataagact tttaaaaagg 1861 gaaagaagaa ggagtcacct gtaatctctg gaggaatact agaagtgttc atcaaagagg 1921 caaagaattt gacagcagtg aagtcaggag gcacttctga tagctttgtg aagggctacc 1981 tgctccctga tgatagcaaa gccaccaagc acaaaactct ggtaataaaa aagagtgtta 2041 acccTCAGTG GAATCATACA TTCATGTTCA GTGGCATCCA TCCCCAGGAT ATAAAGAATG 2101 TTTGCCTAGA ACTTACTATC TGGGACAAGG AGGCCTTTTC CAGCAACATC TTTCTGGGAG 2161 GAGTTCGTTT GAATTCTGGA AGTGGTGTGA GCCATGGGAA GAACGTGGAT TGGATGGACT 2221 CTCAGGGGGA AGAGCAGCGC CTTTGGCAGA AGATGGCCAA CAACCCTGGA ACTCCCTTTG 2281 AGGGTGTACT CATGCTTCGT TCCAGCATGG GAAAATGTAG GCTCTTGCCA ACTTTCTTGT 2341 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2401 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2461 GAAAGGACGA GAGCAAATTC GCCGCCAGCT TATAACGCGT TAAGTCgaca atcaacctct 2521 ggattacaaa atttgtgaaa gatt