Construct: ORF TRCN0000491288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019750.2_s317c1
- DNA Barcode:
- TAGGGCGCTACTTAGAGATGACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM4 (1132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1132 | CHRM4 | cholinergic receptor muscar... | NM_000741.5 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 1132 | CHRM4 | cholinergic receptor muscar... | NM_001366692.1 | 99.7% | 99.7% | (many diffs) |
| 3 | mouse | 12672 | Chrm4 | cholinergic receptor, musca... | NM_007699.2 | 89.2% | 95.6% | (many diffs) |
| 4 | mouse | 12672 | Chrm4 | cholinergic receptor, musca... | XM_006498654.2 | 85.8% | 92% | (many diffs) |
| 5 | mouse | 12672 | Chrm4 | cholinergic receptor, musca... | XM_011239271.2 | 67.1% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1509
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccaacttc acacctgtca atggcagctc tggcaatcag tccgtgcgcc 121 tggtcacgtc atcatcccac aatcgctatg agacggtgga aatggtcttc attgccacag 181 tgacaggctc cctgagcctg gtgactgtcg tgggcaacat cctggtgatg ctgtccatca 241 aggtcaacag gcagctgcag acagtcaaca actacttcct cttcagcctg gcgtgtgctg 301 atctcatcat aggcgccttc tccatgaacc tctacaccgt gtacatcatc aagggctact 361 ggcccctggg cgccgtggtc tgcgacctgt ggctggccct ggactacgtg gtgagcaacg 421 cctccgtcat gaaccttctc atcatcagct ttgaccgcta cttctgcgtc accaagcctc 481 tcacctaccc tgcccggcgc accaccaaga tggcaggcct catgattgct gctgcctggg 541 tactgtcctt cgtgctctgg gcgcctgcca tcttgttctg gcagtttgtg gtgggtaagc 601 ggacggtgcc cgacaaccag tgcttcatcc agttcctgtc caacccagca gttacctttg 661 gcacagccat tgctgccttc tacctgcctg tggtcatcat gacggtgctg tacatccaca 721 tctccctggc cagtcgcagc cgagtccaca agcaccggcc cgagggcccg aaggagaaga 781 aagccaagac gctggccttc ctcaagagcc cactaatgaa gcagagcgtc aagaagcccc 841 cgcccgggga ggccgcccgt gaggagctgc gcaatggcaa gctggaggag gcccccccgc 901 cagcgctgcc accgccaccg cgccccgtgg ctgataagga cacttccaat gagtccagct 961 caggcagtgc cacccagaac accaaggaac gcccagccac agagctgtcc accacagagg 1021 ccaccacgcc cgccatgccc gcccctcccc tgcagccgcg ggccctcaac ccagcctcca 1081 gatggtccaa gatccagatt gtgacgaagc agacaggcaa tgagtgtgtg acagccattg 1141 agattgtgcc tgccacgccg gctggcatgc gcccTGCGGC CAACGTGGCC CGCAAGTTCG 1201 CCAGCATCGC TCGCAACCAG GTGCGCAAGA AGCGGCAGAT GGCGGCCCGG GAGCGCAAAG 1261 TGACACGAAC GATCTTTGCC ATTCTGCTAG CCTTCATCCT CACCTGGACG CCCTACAACG 1321 TCATGGTCCT GGTGAACACC TTCTGCCAGA GCTGCATCCC TGACACGGTG TGGTCCATTG 1381 GCTACTGGCT CTGCTACGTC AACAGCACCA TCAACCCTGC CTGCTATGCT CTGTGCAACG 1441 CCACCTTTAA AAAGACCTTC CGGCACCTGC TGCTGTGCCA GTATCGGAAC ATCGGCACTG 1501 CCAGGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATAGGGCGCT ACTTAGAGAT GACAGACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt