Construct: ORF TRCN0000491305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003857.1_s317c1
- Derived from:
- ccsbBroadEn_10202
- DNA Barcode:
- GCGGGACACACGCACGAGTTCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BORCS8 (729991)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 729991 | BORCS8 | BLOC-1 related complex subu... | NM_001145784.2 | 99.7% | 100% | 81G>A |
| 2 | human | 729991 | BORCS8 | BLOC-1 related complex subu... | NM_001145783.2 | 91% | 90.7% | 81G>A;327_327delGins31 |
| 3 | human | 4207 | BORCS8-MEF2B | BORCS8-MEF2B readthrough | NR_027308.2 | 21% | 1_35del;116G>A;393_1691del | |
| 4 | human | 4207 | BORCS8-MEF2B | BORCS8-MEF2B readthrough | NR_027307.2 | 19.7% | 1_35del;116G>A;393_1803del | |
| 5 | mouse | 72368 | Borcs8 | BLOC-1 related complex subu... | NM_001145552.2 | 85.2% | 94.1% | (many diffs) |
| 6 | mouse | 72368 | Borcs8 | BLOC-1 related complex subu... | NM_001309841.1 | 80% | 84.1% | (many diffs) |
| 7 | mouse | 72368 | Borcs8 | BLOC-1 related complex subu... | NM_001309645.1 | 79.8% | 89% | (many diffs) |
| 8 | mouse | 72368 | Borcs8 | BLOC-1 related complex subu... | NR_132131.1 | 27.7% | (many diffs) | |
| 9 | mouse | 72368 | Borcs8 | BLOC-1 related complex subu... | NR_132132.1 | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagccggag atgcagctca aggGGAAGAA AGTCACGGAC AAGTTCACTG 121 AGAGCGTCTA CGTCCTGGCC AACGAACCAT CCGTGGCCCT GTACCGGCTG CAGGAGCATG 181 TGCGTCGCTC CCTCCCCGAG CTGGCCCAGC ACAAGGCAGA CATGCAGCGT TGGGAGGAGC 241 AGAGCCAGGG AGCCATCTAC ACTGTGGAGT ACGCCTGCAG CGCCGTGAAG AACCTGGTGG 301 ACAGCAGCGT CTACTTCCGC AGCGTGGAGG GTCTGCTCAA ACAGGCCATC AGCATCCGGG 361 ACCATATGAA TGCCAGTGCC CAGGGCCACA GCCCGGAGGA ACCACCCCCG CCCTCCTCAG 421 CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGAGC GGGACACACG CACGAGTTCC TTACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt