Construct: ORF TRCN0000491322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009802.1_s317c1
- Derived from:
- ccsbBroadEn_14272
- DNA Barcode:
- GACTTGCGCCGCTCCAACTCGGGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMC5 (79838)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491322
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_024780.5 | 99.9% | 99.7% | 2274delA |
| 2 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001105248.1 | 71.4% | 63.2% | (many diffs) |
| 3 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001261841.2 | 71.4% | 63.2% | (many diffs) |
| 4 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001308161.1 | 66.4% | 58.3% | (many diffs) |
| 5 | human | 79838 | TMC5 | transmembrane channel like 5 | NM_001105249.1 | 65.8% | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2346
- ORF length:
- 2280
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtccgatgac cacgtgaatg aaatcatcat acaggttgag aatgtttcct 121 ctggggtcca aagccaccca tcctcaaatc agatttttca agaaaaggtg ctgctagact 181 caagcatcaa catggttttg tcaatatctg acattgatgt gatagactct cagacagtca 241 gcaaaaggaa tgaccaaaag ggtaaccagg tgctgcggtt ttcaacatct ttgaatgagt 301 cgatgtctca gacccttcat agcctagaat gcatgggcat agacactcct ggttcttcac 361 atgaaactgt tcaaggacag aagttaatcg catcccttat acccatgaca tccagagaca 421 gaattaaagc catcaggaac cagccaagga ccatggaaga gaaaaggaac cttaggaaaa 481 tagttgacaa agaaaaaagc aaacagaccc atcgtatcct tcagctcaat tgctgtattc 541 agtgtctgaa ctccatttcc cgggcttatc ggagatccaa gaacagcctg tcggaaattc 601 tgaattccat cagcctgtgg cagaagacgc tgaagatcat tggaggcaag tttggaacca 661 gcgtcctctc ctatttcaac tttctgagat ggcttttgaa gttcaacatt ttctcattca 721 tcctgaactt cagcttcatc ataatccctc agtttaccgt ggccaaaaag aacaccctcc 781 agttcactgg gctggagttt ttcactgggg tgggttattt tagggacaca gtgatgtact 841 atggctttta caccaattcc accatccagc acgggaacag cggggcatcc tacaacatgc 901 agctggccta catcttcaca atcggagcat gcttgaccac ctgcttcttc agtttgctgt 961 tcagcatggc caagtatttc cggaacaact tcattaatcc ccacatttac tccggaggga 1021 tcaccaagct gatcttttgc tgggacttca ctgtcactca tgaaaaagct gtgaagctaa 1081 aacagaagaa tcttagcact gagataaggg agaacctgtc agagctccgt caggagaatt 1141 ccaagttgac gttcaatcag ctgctgaccc gcttctctgc ctacatggta gcctgggttg 1201 tctctacagg agtggccata gcctgctgtg cagccgttta ttacctggct gagtacaact 1261 tagagttcct gaagacacac agtaaccctg gggcggtgct gttactgcct ttcgttgtgt 1321 cctgcattaa tctggccgtg ccatgcatct actccatgtt caggcttgtg gagaggtacg 1381 agatgccacg gcacgaagtc tacgttctcc tgatccgaaa catctttttg aaaatatcaa 1441 tcattggcat tctttgttac tattggctca acaccgtggc cctgtctggt gaagagtgtt 1501 gggaaaccct cattggccag gacatctacc ggctccttct gatggatttt gtgttctctt 1561 tagtcaattc cttcctgggg gagtttctga ggagaatcat tgggatgcaa ctgatcacaa 1621 gtcttggcct tcaggagttt gacattgcca ggaacgttct agaactgatc tatgcacaaa 1681 ctctggtgtg gattggcatc ttcttctgcc ccctgctgcc ctttatccaa atgattatgc 1741 ttttcatcat gttctactcc aaaaatatca gcctgatgat gaatttccag cctccgagca 1801 aagcctggcg ggcctcacag atgatgactt tcttcatctt cttgctcttt ttcccatcct 1861 tcaccggggt cttgtgcacc ctggccatca ccatctggag attgaagcct tcagctgact 1921 gtggcccttt tcgaggtctg cctctcttca ttcactccat ctacagctgg atcgacaccc 1981 taagtacacg gcctggctac ctgtgggttg tttggatcta tcggaacctc attGGAAGTG 2041 TGCACTTCTT TTTCATCCTC ACCCTCATTG TGCTAATCAT CACCTATCTT TACTGGCAGA 2101 TCACAGAGGG AAGGAAGATT ATGATAAGGC TGCTCCATGA GCAGATCATT AATGAGGGCA 2161 AAGATAAAAT GTTCCTGATA GAAAAATTGA TCAAGCTGCA GGATATGGAG AAGAAAGCAA 2221 ACCCCAGCTC ACTTGTTCTG GAAAGGAGAG AGGTGGAGCA ACAAGGCTTT TTGCATTTGG 2281 GGGAACATGA TGGCAGTCTT GACTTGCGAT CTAGAAGATC AGTTCAAGAA GGTAATCCAG 2341 GGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 2401 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 2461 TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACTTGCGCC GCTCCAACTC GGGCACGCGT 2521 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt