Construct: ORF TRCN0000491338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020810.2_s317c1
- DNA Barcode:
- ACGTTATTCAATCGTGCCACTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADORA1 (134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491338
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134 | ADORA1 | adenosine A1 receptor | NM_000674.3 | 99.8% | 99.6% | 782C>A |
| 2 | human | 134 | ADORA1 | adenosine A1 receptor | NM_001048230.2 | 99.8% | 99.6% | 782C>A |
| 3 | human | 134 | ADORA1 | adenosine A1 receptor | NM_001365065.1 | 74.7% | 60.6% | (many diffs) |
| 4 | human | 134 | ADORA1 | adenosine A1 receptor | XM_005244901.1 | 74.7% | 60.6% | (many diffs) |
| 5 | human | 134 | ADORA1 | adenosine A1 receptor | XM_005244902.4 | 74.7% | 60.6% | (many diffs) |
| 6 | human | 134 | ADORA1 | adenosine A1 receptor | NM_001365066.1 | 64.3% | 64.1% | 0_1ins348;434C>A |
| 7 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001008533.3 | 88.5% | 94.4% | (many diffs) |
| 8 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001039510.2 | 88.5% | 94.4% | (many diffs) |
| 9 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001282945.1 | 88.5% | 94.4% | (many diffs) |
| 10 | mouse | 11539 | Adora1 | adenosine A1 receptor | XM_006529079.2 | 88.5% | 94.4% | (many diffs) |
| 11 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001291928.1 | 61.1% | 60.3% | (many diffs) |
| 12 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001291930.1 | 50.1% | 52.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1050
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgccc tccatctcag ctttccaggc cgcctacatc ggcatcgagg 121 tgctcatcgc cctggtctct gtgcccggga acgtgctggt gatctgggcg gtgaaggtga 181 accaggcgct gcgggatgcc accttctgct tcatcgtgtc gctggcggtg gctgatgtgg 241 ccgtgggtgc cctggtcatc cccctcgcca tcctcatcaa cattgggcca cagacctact 301 tccacacctg cctcatggtt gcctgtccgg tcctcatcct cacccagagc tccatcctgg 361 ccctgctggc aattgctgtg gaccgctacc tccgggtcaa gatccctctc cggtacaaga 421 tggtggtgac cccccggagg gcggcggtgg ccatagccgg ctgctggatc ctctccttcg 481 tggtgggact gacccctatg tttggctgga acaatctgag tgcggtggag cgggcctggg 541 cagccaacgg cagcatgggg gagcccgtga tcaagtgcga gttcgagaag gtcatcagca 601 tggagtacat ggtctacttc aacttctttg tgtgggtgct gcccccgctt ctcctcatgg 661 tcctcatcta cctggaggtc ttctacctaa tccgcaagca gctcaacaag aaggtgtcgg 721 cctccTCCGG CGACCCGCAG AAGTACTATG GGAAGGAGCT GAAGATCGCC AAGTCGCTGG 781 CCCTCATCCT CTTCCTCTTT GCCCTCAGCT GGCTGCCTTT GCACATCCTC AACTGCATCA 841 CCCTCTTCTG CCAGTCCTGC CACAAGCCCA GCATCCTTAC CTACATTGCC ATCTTCCTCA 901 CGCACGGCAA CTCGGCCATG AACCCCATTG TCTATGCCTT CCGCATCCAG AAGTTCCGCG 961 TCACCTTCCT TAAGATTTGG AATGACCATT TCCGCTGCCA GCCTGCACCT CCCATTGACG 1021 AGGATCTCCC AGAAGAGAGG CCTGATGACT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 1201 GTTATTCAAT CGTGCCACTT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt