Construct: ORF TRCN0000491355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019186.2_s317c1
- DNA Barcode:
- GGTGGCATAAGATCCCCTACGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPAN (56342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56342 | PPAN | peter pan homolog | NM_020230.7 | 100% | 100% | |
| 2 | human | 56342 | PPAN | peter pan homolog | NM_001346139.1 | 99.7% | 99.7% | 590_591insTAG |
| 3 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001198690.2 | 90% | 86.9% | (many diffs) |
| 4 | human | 56342 | PPAN | peter pan homolog | NM_001346141.1 | 88.7% | 88.7% | 0_1ins159 |
| 5 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001040664.3 | 56.9% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1494
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggga cagtcaggga ggtcccggca ccagaagcgc gcccgcgccc 121 aggcgcagct ccgcaacctc gaggcctatg ccgcgaaccc gcactcgttc gtgttcacgc 181 gaggctgcac gggtcgcaac atccggcagc tcagcctgga cgtgcggcgg gtcatggagc 241 cgctcactgc cagccgtctg caggttcgta agaagaactc gctgaaggac tgcgtggcag 301 tggctgggcc cctcggggtc acacactttc tgatcctgag caaaacagag accaatgtct 361 actttaagct gatgcgcctc ccaggaggcc ccaccttgac cttccaggtc aagaagtact 421 cgctggtgcg tgatgtggtc tcctcactgc gccggcaccg catgcacgag cagcagtttg 481 cccacccacc cctcctggta ctcaacagct ttggccccca tggtatgcat gtgaagctca 541 tggccaccat gttccagaac ctgttcccct ccatcaacgt gcacaaggtg aacctgaaca 601 ccatcaagcg ctgcctcctc atcgactaca accccgactc ccaggagctg gacttccgcc 661 actatagcat caaagttgtt cctgtgggcg cgagtcgcgg gatgaagaag ctgctccagg 721 agaagttccc caacatgagc cgcctgcagg acatcagcga gctgctggcc acgggcgcgg 781 ggctgtcgga gagcgaggca gagcctgacg gcgaccacaa catcacagag ctgcctcagg 841 ctgtcgctgg ccgtggcaac atgcgggccc agcagagtgc agtgcggctc accgagatcg 901 gcccgcggat gacactgcag ctcatcaagg tccaggaggg cgtcggggag ggcaaagtga 961 tgttccacag ttttgtgagc aagacggagg aggagctgca ggccatcctg gaagccaagg 1021 agaagaagct gcggctgaag gcgcagaggc aggcccagca ggcccagaat gtgcagcgca 1081 agcaggagca gcgggaggcc cacagaaaga agagcctgga gggcatgaag aaggcacggg 1141 tcgggggtag tgatgaagag gcctctggga tcccttcaag gacggcgagc ctGGAGTTGG 1201 GTGAGGACGA TGATGAACAG GAAGATGATG ACATCGAGTA TTTCTGCCAG GCGGTGGGCG 1261 AGGCGCCCAG TGAGGACCTG TTCCCCGAGG CCAAGCAGAA ACGGCTTGCC AAGTCTCCAG 1321 GGCGGAAGCG GAAGCGGTGG GAAATGGATC GAGGCAGGGG TCGCCTTTGT GACCAGAAGT 1381 TTCCCAAGAC CAAGGACAAG TCCCAGGGAG CCCAGGCCAG GCGGGGGCCC AGAGGGGCTT 1441 CCCGGGATGG TGGGCGAGGC CGGGGCCGGG GCCGCCCAGG GAAGAGAGTG GCCGACCCAG 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAG GTGGCATAAG ATCCCCTACG TCAACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att