Construct: ORF TRCN0000491361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014791.1_s317c1
- Derived from:
- ccsbBroadEn_09949
- DNA Barcode:
- ACCGCTAGATCAATTTGGGCTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HID1 (283987)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283987 | HID1 | HID1 domain containing | NM_030630.3 | 99.9% | 100% | 1506T>C;2139T>C |
| 2 | human | 283987 | HID1 | HID1 domain containing | XM_005257226.2 | 99.7% | 99.8% | 386_387insGCA;1503T>C;2136T>C |
| 3 | human | 283987 | HID1 | HID1 domain containing | XR_001752481.2 | 70% | (many diffs) | |
| 4 | human | 283987 | HID1 | HID1 domain containing | XR_001752482.2 | 69.9% | (many diffs) | |
| 5 | human | 283987 | HID1 | HID1 domain containing | XR_001752478.2 | 61.4% | (many diffs) | |
| 6 | human | 283987 | HID1 | HID1 domain containing | XR_001752479.2 | 55.3% | (many diffs) | |
| 7 | human | 283987 | HID1 | HID1 domain containing | XR_001752480.2 | 55.2% | (many diffs) | |
| 8 | mouse | 217310 | Hid1 | HID1 domain containing | NM_175454.2 | 88.8% | 95.6% | (many diffs) |
| 9 | mouse | 217310 | Hid1 | HID1 domain containing | NM_001346774.1 | 88.7% | 95.5% | (many diffs) |
| 10 | mouse | 217310 | Hid1 | HID1 domain containing | XM_017314488.1 | 66.1% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2430
- ORF length:
- 2364
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtcgaccgac tccaagctga acttccggaa ggcggtgatc cagctcacca 121 ccaagacgca gcccgtggaa gccaccgatg atgccttttg ggaccagttc tgggcagaca 181 cagccacctc ggtgcaggat gtgtttgcac tggtgccggc agcagagatc cgggccgtgc 241 gggaagagtc accctccaac ttggccaccc tgtgctacaa ggccgttgag aagctggtgc 301 agggagctga gagtggctgc cactcggaga aggagaagca gatcgtcctg aactgcagcc 361 ggctgctcac ccgcgtgctg ccctacatct ttgaggaccc cgactggagg ggcttcttct 421 ggtccacagt gcccggggca gggcgaggag ggcagggaga agaggatgat gagcatgcca 481 ggcccctggc cgagtccctg ctcctggcca ttgctgacct gctcttctgc ccggacttca 541 cggttcagag ccaccggagg agcactgtgg actcggcaga ggacgtccac tccctggaca 601 gctgtgaata catctgggag gctggtgtgg gcttcgctca ctccccccag cctaactaca 661 tccacgatat gaaccggatg gagctgctga aactgctgct gacatgcttc tccgaggcca 721 tgtacctgcc cccagctccg gaaagtggca gcaccaaccc atgggttcag ttcttttgtt 781 ccacggagaa cagacatgcc ctgcccctct tcacctccct cctcaacacc gtgtgtgcct 841 atgaccctgt gggctacggg atcccctaca accacctgct cttctctgac taccgggaac 901 ccctggtgga ggaggctgcc caggtgctca ttgtcacttt ggaccacgac agtgccagca 961 gtgccagccc cactgtggac ggcaccacca ctggcaccgc catggatgat gccgatcctc 1021 caggccctga gaacctgttt gtgaactacc tgtcccgcat ccatcgtgag gaggacttcc 1081 agttcatcct caagggtata gcccggctgc tgtccaaccc cctgctccag acctacctgc 1141 ctaactccac caagaagatc cagttccacc aggagctgct agttctcttc tggaagctct 1201 gcgacttcaa caagaaattc ctcttcttcg tgctgaagag cagcgacgtc ctagacatcc 1261 ttgtccccat cctcttcttc ctcaacgatg cccgggccga tcagtctcgg gtgggcctga 1321 tgcacattgg tgtcttcatc ttgctgcttc tgagcgggga gcggaacttc ggggtgcggc 1381 tgaacaaacc ctactcaatc cgcgtgccca tggacatccc agtcttcaca gggacccacg 1441 ccgacctgct cattgtggtg ttccacaaga tcatcaccag cgggcaccag cggttgcagc 1501 ccctcttcga ctgcctgctc accatcgtgg tcaacgtgtc cccctacctc aagagcctgt 1561 ccatggtgac cgccaacaag ttgctgcacc tgctggaggc cttctccacc acctggttcc 1621 tcttctctgc cgcccagaac caccacctgg tcttcttcct cctggaggtc ttcaacaaca 1681 tcatccagta ccagtttgat ggcaactcca acctggtcta cgccatcatc cgcaagcgca 1741 gcatcttcca ccagctggcc aacctgccca cggacccgcc caccattcac aaggccctgc 1801 agcggcgccg gcggacacct gagcccttgt ctcgcaccgg ctcccaggag ggcacctcca 1861 tggagggctc ccgccccgct gcccctgcag agccaggcac cctcaagacc agtctggtgg 1921 ctactccagg cattgacaag ctgaccgaga agtcccaggt gtcagaggat ggcaccttgc 1981 ggtccctgga acctgagccc cagcagagct tggaggatgg cagcccggct aagggggagc 2041 ccagccaggc atggagggag cagcggcgac cgtccacctc atcagccagt gggcagtgga 2101 gcccaacgcc agagtgggTC CTCTCCTGGA AGTCGAAGCT GCCGCTGCAG ACCATCATGA 2161 GGCTGCTGCA GGTGCTGGTT CCGCAGGTGG AGAAGATCTG CATCGACAAG GGCCTGACGG 2221 ATGAGTCTGA GATCCTGCGG TTCCTGCAGC ATGGCACCCT GGTGGGGCTG CTGCCCGTGC 2281 CCCACCCCAT CCTCATCCGC AAGTACCAGG CCAACTCGGG CACTGCCATG TGGTTCCGCA 2341 CCTACATGTG GGGCGTCATC TATCTGAGGA ATGTGGACCC CCCTGTCTGG TACGACACCG 2401 ACGTGAAGCT GTTTGAGATA CAGCGGGTGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2461 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2521 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACCGC 2581 TAGATCAATT TGGGCTTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2641 tgaaagatt