Construct: ORF TRCN0000491367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013332.1_s317c1
- Derived from:
- ccsbBroadEn_12578
- DNA Barcode:
- TCATTATCGATCAAGCTCGGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCRN3 (79634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79634 | SCRN3 | secernin 3 | XM_011511839.3 | 64.5% | 64.5% | 1_366del |
| 2 | human | 79634 | SCRN3 | secernin 3 | NM_001193528.1 | 53.2% | 53.2% | 1_585del |
| 3 | human | 79634 | SCRN3 | secernin 3 | NM_024583.5 | 52.3% | 52.3% | 1_606del |
| 4 | human | 79634 | SCRN3 | secernin 3 | XM_005246854.5 | 52.3% | 52.3% | 1_606del |
| 5 | human | 79634 | SCRN3 | secernin 3 | XM_005246855.3 | 52.3% | 52.3% | 1_606del |
| 6 | human | 79634 | SCRN3 | secernin 3 | XM_005246856.3 | 52.3% | 52.3% | 1_606del |
| 7 | human | 79634 | SCRN3 | secernin 3 | XM_005246857.3 | 52.3% | 52.3% | 1_606del |
| 8 | human | 79634 | SCRN3 | secernin 3 | XM_017004910.2 | 52.3% | 52.3% | 1_606del |
| 9 | human | 79634 | SCRN3 | secernin 3 | XM_005246853.3 | 50% | 50% | 1_666del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aaactatgct aagcggaaag gttggtggga tggtaaaaag gagtttgatt 121 ttgctgcagc atattcctat cttgacacag ccaagatgat gacttcatca ggcagatact 181 gtgagggcta caagcttcta aataagcaca aaggaaatat aacttttgaa acaatgatgg 241 aaattcttcg agataaacca agtggcatta atatggaggg agaattcctg accactgcaa 301 gcatggtttc tattttacct caagactcca gccttccttg cattcacttc tttacaggga 361 ctcctgatcc TGAGAGATCT GTTTTTAAGC CTTTCATATT TGTGCCACAT ATTTCACAAC 421 TATTGGATAC CAGTTCACCA ACATTTGAAC TTGAAGATCT AGTTAAAAAG AAATCACATT 481 TTAAGCCTGA CAGAAGACAC CCACTCTACC AAAAACATCA ACAGGCATTG GAAGTAGTAA 541 ATAATAATGA GGAAAAAGCC AAAATAATGT TGGACAACAT GAGGAAACTG GAGAAAGAAC 601 TATTCAGAGA GATGGAATCA ATCCTTCAAA ACAAGCATCT TGATGTGGAG AAAATTGTTA 661 ATCTCTTTCC TCAGTGTACA AAAGATGAAA TTCAAATTTA TCAGTCAAAT TTATCAGTCA 721 AAGTTAGTTC TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA TTATCGATCA AGCTCGGACC 901 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t