Transcript: Human XM_005246853.3

PREDICTED: Homo sapiens secernin 3 (SCRN3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SCRN3 (79634)
Length:
3957
CDS:
444..1778

Additional Resources:

NCBI RefSeq record:
XM_005246853.3
NBCI Gene record:
SCRN3 (79634)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005246853.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000133844 CAACAGTCGATAACAGGATTA pLKO.1 547 CDS 100% 10.800 15.120 N SCRN3 n/a
2 TRCN0000133668 GAGAACGTCTTAAGTGTACAT pLKO.1 649 CDS 100% 4.950 6.930 N SCRN3 n/a
3 TRCN0000133714 GCATTAATATGGAGGGAGAAT pLKO.1 1309 CDS 100% 4.950 3.960 N SCRN3 n/a
4 TRCN0000133880 CAGGCATTGGAAGTAGTAAAT pLKO.1 1566 CDS 100% 13.200 9.240 N SCRN3 n/a
5 TRCN0000134212 CAGGTGATGTACTTGCATTAT pLKO.1 2008 3UTR 100% 13.200 9.240 N SCRN3 n/a
6 TRCN0000134628 GAGATAAACCAAGTGGCATTA pLKO.1 1294 CDS 100% 10.800 7.560 N SCRN3 n/a
7 TRCN0000135358 GATGACTTCATCAGGCAGATA pLKO.1 1202 CDS 100% 4.950 3.465 N SCRN3 n/a
8 TRCN0000136102 GCCTTTCATATTTGTGCCACA pLKO.1 1433 CDS 100% 2.160 1.512 N SCRN3 n/a
9 TRCN0000134849 CAGACATGAGAAACTATGCTA pLKO.1 1105 CDS 100% 3.000 1.800 N SCRN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005246853.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12579 pDONR223 100% 93.1% 93.7% None 1_60del;1293_1305del;1332_1333insTAGTGATCATATGGTCAGC n/a
2 TRCN0000469027 CTATCTAAACTCATGATACTCAAT pLX_317 33.6% 92.4% 93.7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_12578 pDONR223 100% 50% 50% None 1_666del n/a
4 ccsbBroad304_12578 pLX_304 0% 50% 50% V5 1_666del n/a
5 TRCN0000491367 TCATTATCGATCAAGCTCGGACCC pLX_317 54.2% 50% 50% V5 1_666del n/a
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