Transcript: Human NM_024583.5

Homo sapiens secernin 3 (SCRN3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-17
Taxon:
Homo sapiens (human)
Gene:
SCRN3 (79634)
Length:
3052
CDS:
98..1372

Additional Resources:

NCBI RefSeq record:
NM_024583.5
NBCI Gene record:
SCRN3 (79634)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024583.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000133844 CAACAGTCGATAACAGGATTA pLKO.1 141 CDS 100% 10.800 15.120 N SCRN3 n/a
2 TRCN0000133668 GAGAACGTCTTAAGTGTACAT pLKO.1 243 CDS 100% 4.950 6.930 N SCRN3 n/a
3 TRCN0000133714 GCATTAATATGGAGGGAGAAT pLKO.1 903 CDS 100% 4.950 3.960 N SCRN3 n/a
4 TRCN0000133880 CAGGCATTGGAAGTAGTAAAT pLKO.1 1160 CDS 100% 13.200 9.240 N SCRN3 n/a
5 TRCN0000134212 CAGGTGATGTACTTGCATTAT pLKO.1 1602 3UTR 100% 13.200 9.240 N SCRN3 n/a
6 TRCN0000134628 GAGATAAACCAAGTGGCATTA pLKO.1 888 CDS 100% 10.800 7.560 N SCRN3 n/a
7 TRCN0000135358 GATGACTTCATCAGGCAGATA pLKO.1 796 CDS 100% 4.950 3.465 N SCRN3 n/a
8 TRCN0000136102 GCCTTTCATATTTGTGCCACA pLKO.1 1027 CDS 100% 2.160 1.512 N SCRN3 n/a
9 TRCN0000134849 CAGACATGAGAAACTATGCTA pLKO.1 699 CDS 100% 3.000 1.800 N SCRN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024583.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12579 pDONR223 100% 97.5% 98.1% None 1233_1245del;1272_1273insTAGTGATCATATGGTCAGC n/a
2 TRCN0000469027 CTATCTAAACTCATGATACTCAAT pLX_317 33.6% 96.7% 98.1% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_12578 pDONR223 100% 52.3% 52.3% None 1_606del n/a
4 ccsbBroad304_12578 pLX_304 0% 52.3% 52.3% V5 1_606del n/a
5 TRCN0000491367 TCATTATCGATCAAGCTCGGACCC pLX_317 54.2% 52.3% 52.3% V5 1_606del n/a
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