Construct: ORF TRCN0000491372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006888.1_s317c1
- Derived from:
- ccsbBroadEn_10405
- DNA Barcode:
- AAACACTGATCAGAGAAGGTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC727751 (727751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84559 | GOLGA2P2Y | GOLGA2 pseudogene 2 Y-linked | NR_001555.2 | 22.8% | (many diffs) | |
| 2 | human | 401634 | GOLGA2P3Y | GOLGA2 pseudogene 3 Y-linked | NR_002195.2 | 22.8% | (many diffs) | |
| 3 | human | 101929479 | LOC101929479 | golgin A2 pseudogene | NR_158179.1 | 16.7% | (many diffs) | |
| 4 | human | 101929479 | LOC101929479 | golgin A2 pseudogene | NR_160936.1 | 12.1% | (many diffs) | |
| 5 | human | 80154 | GOLGA2P10 | GOLGA2 pseudogene 10 | NR_026811.3 | 8.2% | 1_381del;640_3114del | |
| 6 | human | 727751 | LOC727751 | golgin A2 pseudogene | NR_102748.1 | 8.2% | (many diffs) | |
| 7 | human | 102724135 | LOC102724135 | uncharacterized LOC102724135 | NR_158192.1 | 8% | (many diffs) | |
| 8 | human | 80154 | GOLGA2P10 | GOLGA2 pseudogene 10 | NR_103496.2 | 8% | (many diffs) | |
| 9 | human | 729668 | GOLGA2P6 | GOLGA2 pseudogene 6 | NR_120609.1 | 7.8% | (many diffs) | |
| 10 | human | 2801 | GOLGA2 | golgin A2 | XM_005251932.1 | 7.7% | 6.2% | (many diffs) |
| 11 | human | 2801 | GOLGA2 | golgin A2 | NM_001366246.1 | 7.6% | 6.1% | (many diffs) |
| 12 | human | 2801 | GOLGA2 | golgin A2 | NM_004486.5 | 7.6% | 6.1% | (many diffs) |
| 13 | human | 2801 | GOLGA2 | golgin A2 | NM_001366244.1 | 7.4% | 5.9% | (many diffs) |
| 14 | human | 80154 | GOLGA2P10 | GOLGA2 pseudogene 10 | NR_033936.3 | 7.2% | (many diffs) | |
| 15 | human | 388152 | GOLGA2P7 | GOLGA2 pseudogene 7 | NR_027001.1 | 7.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 348
- ORF length:
- 258
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCGAACAT CCCAGGGGAC CTGGAGAGCC 121 GGGAGGCCAT GGTGGCATTT TTCAACTCCG CTGGAGCCAA TGCCCAGGAG GAACAAAGGG 181 TGTGCTGCCA GCCCCTGGCT CACCCAGTGG CCTCGTCCCA GAAAAAGCCA GAGGTAGCGG 241 CCCCAGCCCC AGAGAGTGGG GGTGAGTCTG TGTTTGGGGA GACCCACCGG GCCCTGCAGG 301 GGGCCATGGA GAAGCTGCAG CGACTTTATG GAAGGAGAAG GTGGACCTGC CCAACTTTCT 361 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 421 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA AAACACTGAT CAGAGAAGGT GCCAACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt