Construct: ORF TRCN0000491376
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020313.2_s317c1
- DNA Barcode:
- TTAACGGTAGTAGCGGATTGGAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RIOK3 (8780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491376
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8780 | RIOK3 | RIO kinase 3 | NM_003831.5 | 100% | 100% | |
| 2 | human | 8780 | RIOK3 | RIO kinase 3 | NM_001348193.2 | 96.9% | 96.9% | 179_180ins48 |
| 3 | human | 8780 | RIOK3 | RIO kinase 3 | XM_011526242.1 | 51.6% | 47.4% | 0_1ins625;62_63ins128 |
| 4 | human | 8780 | RIOK3 | RIO kinase 3 | XM_011526243.1 | 50.6% | 37.8% | 0_1ins625;241_242ins143 |
| 5 | human | 8780 | RIOK3 | RIO kinase 3 | NR_145477.2 | 43.2% | 1_153del;1406_1427del;1733_3596del | |
| 6 | human | 8780 | RIOK3 | RIO kinase 3 | XR_002958189.1 | 34.2% | (many diffs) | |
| 7 | mouse | 66878 | Riok3 | RIO kinase 3 | NM_024182.4 | 89.9% | 93.6% | (many diffs) |
| 8 | mouse | 66878 | Riok3 | RIO kinase 3 | XM_017317969.1 | 34.6% | 36.4% | (many diffs) |
| 9 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782390.1 | 24.4% | (many diffs) | |
| 10 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782391.1 | 23.3% | (many diffs) | |
| 11 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782389.1 | 22.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1629
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatctg gtaggagtgg catcgcctga gcccgggacg gcagcggcct 121 ggggacccag caagtgtcca tgggctattc ctcaaaatac aatatcttgt tctttggctg 181 atgtaatgag tgaacagctg gccaaagaat tgcagttaga agaagaagct gccgtttttc 241 ctgaagttgc tgttgctgaa ggaccattta ttactggaga aaacattgat acttccagtg 301 accttatgct ggctcagatg ctacagatgg aatatgacag agaatatgat gcacagctta 361 ggcgtgaaga aaaaaaattc aatggagata gcaaagtttc catttccttt gaaaattatc 421 gaaaagtgca tccttatgaa gacagcgata gctctgaaga tgaggttgac tggcaggata 481 ctcgtgatga tccctacaga ccagcaaaac cggttcccac tcctaaaaag ggctttattg 541 gaaaaggaaa agatatcacc accaaacatg atgaagtagt atgtgggaga aagaacacag 601 caagaatgga aaattttgca cctgagtttc aggtaggaga tggaattgga atggatttaa 661 aactatcaaa ccatgttttc aatgctttaa aacaacatgc ctactcagaa gaacgtcgaa 721 gtgcccgcct acatgagaaa aaggagcatt ctacagcaga aaaagcagtt gatcctaaga 781 cacgtttact tatgtataaa atggtcaact ctggaatgtt ggagacaatc actggctgta 841 ttagtacagg aaaggagtct gttgtctttc atgcatatgg agggagcatg gaggatgaaa 901 aggaagatag taaagttata cctacagaat gtgccatcaa ggtatttaaa acaaccctta 961 atgaatttaa gaatcgtgac aaatatatta aagatgattt caggtttaaa gatcgcttca 1021 gtaaactaaa tccacgtaag atcatccgca tgtgggcaga aaaagaaatg cacaatctcg 1081 caagaatgca gagagctgga attccttgtc caacagttgt actactgaag aaacacattt 1141 tagttatgtc ttttattggc catgatcaag ttccagcccc taaattaaaa gaagtaaagc 1201 tcaatagtga agaaatgaaa gaagcctact atcaaactct tcatttgatg cggcagttat 1261 atcatgaatg tacgcttgtc catgctgacc tcagtgagta taacatgctg tggcatgctg 1321 gaaaggtctg gttgatcgat gtcagtcagt cagtagaacc tacccaccct cacggcctgg 1381 agttcttgtt ccgggactgc aggaatgtct cgcagttttt cCAGAAAGGA GGAGTCAAGG 1441 AAGCCCTTAG TGAACGAGAA CTCTTCAATG CTGTTTCAGG CTTAAACATC ACAGCAGATA 1501 ATGAAGCTGA TTTTTTAGCT GAGATAGAAG CTTTGGAGAA AATGAATGAA GATCACGTTC 1561 AGAAGAATGG AAGGAAAGCT GCTTCATTTT TGAAAGATGA TGGAGACCCA CCACTACTAT 1621 ATGATGAATG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1741 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTA ACGGTAGTAG CGGATTGGAG 1801 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t