Construct: ORF TRCN0000491389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019277.2_s317c1
- DNA Barcode:
- CACACCTTTGTATTCTATGAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WLS (79971)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79971 | WLS | Wnt ligand secretion mediator | NM_024911.7 | 99.8% | 99.8% | 432G>A;1623_1624insG |
| 2 | human | 79971 | WLS | Wnt ligand secretion mediator | XM_011542191.2 | 93.5% | 93.2% | (many diffs) |
| 3 | human | 79971 | WLS | Wnt ligand secretion mediator | NM_001002292.3 | 93.2% | 92.8% | (many diffs) |
| 4 | human | 79971 | WLS | Wnt ligand secretion mediator | XM_017002390.2 | 91.5% | 91.5% | 0_1ins135;297G>A;1488_1489insG |
| 5 | human | 79971 | WLS | Wnt ligand secretion mediator | XM_011542192.3 | 85.5% | 85% | (many diffs) |
| 6 | human | 79971 | WLS | Wnt ligand secretion mediator | NM_001193334.1 | 83% | 82.8% | 106_107ins273;159G>A;1350_1351insG |
| 7 | mouse | 68151 | Wls | wntless homolog (Drosophila) | NM_026582.4 | 89.5% | 95.9% | (many diffs) |
| 8 | mouse | 68151 | Wls | wntless homolog (Drosophila) | XM_006501964.2 | 89.5% | 95.9% | (many diffs) |
| 9 | mouse | 68151 | Wls | wntless homolog (Drosophila) | NR_037590.1 | 52.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1701
- ORF length:
- 1626
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct ggggcaatta tagaaaacat gagcaccaag aagctgtgca 121 ttgttggtgg gattctgctc gtgttccaaa tcatcgcctt tctggtggga ggcttgattg 181 ctccagggcc cacaacggca gtgtcctaca tgtcggtgaa atgtgtggat gcccgtaaga 241 accatcacaa gacaaaatgg ttcgtgcctt ggggacccaa tcattgtgac aagatccgag 301 acattgaaga ggcaattcca agggaaattg aagccaatga catcgtgttt tctgttcaca 361 ttcccctccc ccacatggag atgagtcctt ggttccaatt catgctgttt atcctgcagc 421 tggacattgc cttcaagcta aacaaccaaa tcagagaaaa tgcagaagtc tccatggacg 481 tttccctggc ttaccgtgat gacgcatttg ctgagtggac tgaaatggcc catgaaagag 541 taccacggaa actcaaatgc accttcacat ctcccaagac tccagagcat gagggccgtt 601 actatgaatg tgatgtcctt cctttcatgg aaattgggtc tgtggcccat aagttttacc 661 ttttaaacat ccggctgcct gtgaatgaga agaagaaaat caatgtggga attggggaga 721 taaaggatat ccggttggtg gggatccacc aaaatggagg cttcaccaag gtgtggtttg 781 ccatgaagac cttccttacg cccagcatct tcatcattat ggtgtggtat tggaggagga 841 tcaccatgat gtcccgaccc ccagtgcttc tggaaaaagt catctttgcc cttgggattt 901 ccatgacctt tatcaatatc ccagtggaat ggttttccat cgggtttgac tggacctgga 961 tgctgctgtt tggtgacatc cgacagggca tcttctatgc gatgcttctg tccttctgga 1021 tcatcttctg tggcgagcac atgatggatc agcacgagcg gaaccacatc gcagggtatt 1081 ggaagcaagt cggacccatt gccgttggct ccttctgcct cttcatattt gacatgtgtg 1141 agagaggggt acaactcacg aatcccttct acagtatctg gactacagac attggaacag 1201 agctggccat ggccttcatc atcgtggctg gaatctgcct ctgcctctac ttcctgtttc 1261 tatgcttcat ggtatttcag gtgtttcgga acatcagtgg gaagcagtcc agcctgccag 1321 ctatgagcaa agtccggcgg ctacactatg aggggctaat ttttaggttc aagttcctca 1381 tgcttatcac cttggcctgc gctgccatga ctgtcatctt cttcatcgtt agtcaggtaa 1441 cggaaggcca ttggaaatgg ggcggcgtca cagtccaagt gaacagtgcc tttttcacag 1501 gcatcTATGG GATGTGGAAT CTGTATGTCT TTGCTCTGAT GTTCTTGTAT GCACCATCCC 1561 ATAAAAACTA TGGAGAAGAC CAGTCCAATG GCGATCTGGG TGTCCATAGT GGGGAAGAAC 1621 TCCAGCTCAC CACCACTATC ACCCATGTGG ACGGACCCAC TGAGATCTAC AAGTTGACCC 1681 GCAAGGAGGC CCAGGAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1741 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1801 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACACCT TTGTATTCTA 1861 TGAAAAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt