Transcript: Mouse NM_026582.4

Mus musculus wntless homolog (Drosophila) (Wls), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Wls (68151)
Length:
2696
CDS:
248..1873

Additional Resources:

NCBI RefSeq record:
NM_026582.4
NBCI Gene record:
Wls (68151)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026582.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234928 AGTACCCTACACGGCAATAAA pLKO_005 373 CDS 100% 15.000 21.000 N Wls n/a
2 TRCN0000234930 GAGCACGAAGGTCGTTATTAT pLKO_005 758 CDS 100% 15.000 21.000 N Wls n/a
3 TRCN0000234931 TGCAGCTTACTACCACTATAA pLKO_005 1794 CDS 100% 13.200 18.480 N Wls n/a
4 TRCN0000234929 GTCCGTAAGAACCACCATAAA pLKO_005 404 CDS 100% 13.200 9.240 N Wls n/a
5 TRCN0000234932 CCTAGTGCTTCTTAGGTATAG pLKO_005 1972 3UTR 100% 10.800 7.560 N Wls n/a
6 TRCN0000133999 CTGCCTCTTCATATTTGACAT pLKO.1 1288 CDS 100% 4.950 3.465 N WLS n/a
7 TRCN0000138115 GTCATCTTTGCCCTTGGGATT pLKO.1 1052 CDS 100% 4.050 2.835 N WLS n/a
8 TRCN0000138094 GTATTGGAGGAGGATCACCAT pLKO.1 1000 CDS 100% 2.640 1.848 N WLS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026582.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491389 CACACCTTTGTATTCTATGAAAAG pLX_317 12.2% 89.5% 95.9% V5 (many diffs) n/a
2 TRCN0000488049 TAGGCGGCAACGACACCTTGACTC pLX_317 19% 89.5% 96.1% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_12651 pDONR223 100% 73.6% 79.8% None (many diffs) n/a
4 ccsbBroad304_12651 pLX_304 0% 73.6% 79.8% V5 (many diffs) n/a
5 TRCN0000467337 CACGTATATACCTACTTTCAATTG pLX_317 30.3% 73.6% 79.8% V5 (many diffs) n/a
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