Construct: ORF TRCN0000491401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002166.2_s317c1
- Derived from:
- ccsbBroadEn_14558
- DNA Barcode:
- GTACCTCCCATCCAAAATTATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALM1 (801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 801 | CALM1 | calmodulin 1 | NM_006888.6 | 99.7% | 99.3% | 428T>A |
| 2 | human | 801 | CALM1 | calmodulin 1 | NM_001363670.1 | 99.1% | 98% | 3_5delGCA;431T>A |
| 3 | human | 805 | CALM2 | calmodulin 2 | NM_001743.6 | 85% | 99.3% | (many diffs) |
| 4 | human | 808 | CALM3 | calmodulin 3 | NM_001329922.1 | 81.6% | 99.3% | (many diffs) |
| 5 | human | 808 | CALM3 | calmodulin 3 | NM_005184.4 | 81.6% | 99.3% | (many diffs) |
| 6 | human | 801 | CALM1 | calmodulin 1 | NM_001363669.1 | 75.6% | 75.1% | 0_1ins108;320T>A |
| 7 | human | 805 | CALM2 | calmodulin 2 | NM_001305625.1 | 64.6% | 75.1% | (many diffs) |
| 8 | human | 805 | CALM2 | calmodulin 2 | NM_001305626.1 | 64.6% | 75.1% | (many diffs) |
| 9 | human | 805 | CALM2 | calmodulin 2 | NM_001305624.1 | 64.1% | 74.6% | (many diffs) |
| 10 | human | 808 | CALM3 | calmodulin 3 | NM_001329921.1 | 61.9% | 75.1% | (many diffs) |
| 11 | human | 808 | CALM3 | calmodulin 3 | NM_001329923.1 | 61.9% | 75.1% | (many diffs) |
| 12 | human | 808 | CALM3 | calmodulin 3 | NM_001329924.2 | 61.9% | 75.1% | (many diffs) |
| 13 | human | 808 | CALM3 | calmodulin 3 | NM_001329925.2 | 61.9% | 75.1% | (many diffs) |
| 14 | human | 808 | CALM3 | calmodulin 3 | NM_001329926.2 | 61.9% | 75.1% | (many diffs) |
| 15 | mouse | 12313 | Calm1 | calmodulin 1 | NM_009790.5 | 92.6% | 99.3% | (many diffs) |
| 16 | mouse | 12314 | Calm2 | calmodulin 2 | NM_007589.5 | 82.9% | 99.3% | (many diffs) |
| 17 | mouse | 12315 | Calm3 | calmodulin 3 | NM_007590.3 | 82.5% | 99.3% | (many diffs) |
| 18 | mouse | 12313 | Calm1 | calmodulin 1 | NM_001313934.1 | 69.1% | 74.6% | (many diffs) |
| 19 | mouse | 12314 | Calm2 | calmodulin 2 | NM_001355703.1 | 63.5% | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgatcagctg accgaagaac agattgctga attcaaggaa gccttctccc 121 tatttgataa agatggcgat ggcaccatca caacaaagga acttggaact gtcatgaggt 181 cactgggtca gaacccaaca gaagctgaat tgcaggatat gatcaatgaa gtggatgctg 241 atggtaatgg caccattgac ttccccgaat ttttgactat gatggctaga aaaatgaaag 301 atacagatag tgaagaagaa atccgtgagg cattccgagt ctttgacaag gatggcaatg 361 gttatatcag tgcagcagaa ctacgtcacg tcatgacaaa cttaggagaa aaactaacag 421 atgaagaagt agatgaaatg atcagagaag cagatattga tGGAGACGGA CAAGTCAACT 481 ATGAAGAATT CGAACAGATG ATGACTGCAA AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 661 ACCTCCCATC CAAAATTATC TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt