Construct: ORF TRCN0000491410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020649.2_s317c1
- DNA Barcode:
- ACTATATTCATACGTACTTCCCGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- INSR (3643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3643 | INSR | insulin receptor | NM_000208.4 | 99.9% | 99.9% | 5C>G |
| 2 | human | 3643 | INSR | insulin receptor | XM_011527988.2 | 99.9% | 99.7% | 5C>G;2842_2843insTAG |
| 3 | human | 3643 | INSR | insulin receptor | NM_001079817.3 | 99.1% | 99% | 5C>G;2229_2230ins36 |
| 4 | human | 3643 | INSR | insulin receptor | XM_011527989.3 | 99% | 98.9% | 5C>G;2229_2230ins36;2806_2807insTAG |
| 5 | mouse | 16337 | Insr | insulin receptor | NM_001330056.1 | 87% | 95.1% | (many diffs) |
| 6 | mouse | 16337 | Insr | insulin receptor | XM_006508700.1 | 86.9% | 95% | (many diffs) |
| 7 | mouse | 16337 | Insr | insulin receptor | NM_010568.3 | 86.2% | 94.4% | (many diffs) |
| 8 | mouse | 16337 | Insr | insulin receptor | XM_006508701.1 | 86.1% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 4230
- ORF length:
- 4146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgggca ccgggggccg gcggggggcg gcggccgcgc 121 cgctgctggt ggcggtggcc gcgctgctac tgggcgccgc gggccacctg taccccggag 181 aggtgtgtcc cggcatggat atccggaaca acctcactag gttgcatgag ctggagaatt 241 gctctgtcat cgaaggacac ttgcagatac tcttgatgtt caaaacgagg cccgaagatt 301 tccgagacct cagtttcccc aaactcatca tgatcactga ttacttgctg ctcttccggg 361 tctatgggct cgagagcctg aaggacctgt tccccaacct cacggtcatc cggggatcac 421 gactgttctt taactacgcg ctggtcatct tcgagatggt tcacctcaag gaactcggcc 481 tctacaacct gatgaacatc acccggggtt ctgtccgcat cgagaagaac aatgagctct 541 gttacttggc cactatcgac tggtcccgta tcctggattc cgtggaggat aattacatcg 601 tgttgaacaa agatgacaac gaggagtgtg gagacatctg tccgggtacc gcgaagggca 661 agaccaactg ccccgccacc gtcatcaacg ggcagtttgt cgaacgatgt tggactcata 721 gtcactgcca gaaagtttgc ccgaccatct gtaagtcaca cggctgcacc gccgaaggcc 781 tctgttgcca cagcgagtgc ctgggcaact gttctcagcc cgacgacccc accaagtgcg 841 tggcctgccg caacttctac ctggacggca ggtgtgtgga gacctgcccg cccccgtact 901 accacttcca ggactggcgc tgtgtgaact tcagcttctg ccaggacctg caccacaaat 961 gcaagaactc gcggaggcag ggctgccacc agtacgtcat tcacaacaac aagtgcatcc 1021 ctgagtgtcc ctccgggtac acgatgaatt ccagcaactt gctgtgcacc ccatgcctgg 1081 gtccctgtcc caaggtgtgc cacctcctag aaggcgagaa gaccatcgac tcggtgacgt 1141 ctgcccagga gctccgagga tgcaccgtca tcaacgggag tctgatcatc aacattcgag 1201 gaggcaacaa tctggcagct gagctagaag ccaacctcgg cctcattgaa gaaatttcag 1261 ggtatctaaa aatccgccga tcctacgctc tggtgtcact ttccttcttc cggaagttac 1321 gtctgattcg aggagagacc ttggaaattg ggaactactc cttctatgcc ttggacaacc 1381 agaacctaag gcagctctgg gactggagca aacacaacct caccatcact caggggaaac 1441 tcttcttcca ctataacccc aaactctgct tgtcagaaat ccacaagatg gaagaagttt 1501 caggaaccaa ggggcgccag gagagaaacg acattgccct gaagaccaat ggggaccagg 1561 catcctgtga aaatgagtta cttaaatttt cttacattcg gacatctttt gacaagatct 1621 tgctgagatg ggagccgtac tggccccccg acttccgaga cctcttgggg ttcatgctgt 1681 tctacaaaga ggccccttat cagaatgtga cggagttcga cgggcaggat gcgtgtggtt 1741 ccaacagttg gacggtggta gacattgacc cacccctgag gtccaacgac cccaaatcac 1801 agaaccaccc agggtggctg atgcggggtc tcaagccctg gacccagtat gccatctttg 1861 tgaagaccct ggtcaccttt tcggatgaac gccggaccta tggggccaag agtgacatca 1921 tttatgtcca gacagatgcc accaacccct ctgtgcccct ggatccaatc tcagtgtcta 1981 actcatcatc ccagattatt ctgaagtgga aaccaccctc cgaccccaat ggcaacatca 2041 cccactacct ggttttctgg gagaggcagg cggaagacag tgagctgttc gagctggatt 2101 attgcctcaa agggctgaag ctgccctcga ggacctggtc tccaccattc gagtctgaag 2161 attctcagaa gcacaaccag agtgagtatg aggattcggc cggcgaatgc tgctcctgtc 2221 caaagacaga ctctcagatc ctgaaggagc tggaggagtc ctcgtttagg aagacgtttg 2281 aggattacct gcacaacgtg gttttcgtcc ccagaaaaac ctcttcaggc actggtgccg 2341 aggaccctag gccatctcgg aaacgcaggt cccttggcga tgttgggaat gtgacggtgg 2401 ccgtgcccac ggtggcagct ttccccaaca cttcctcgac cagcgtgccc acgagtccgg 2461 aggagcacag gccttttgag aaggtggtga acaaggagtc gctggtcatc tccggcttgc 2521 gacacttcac gggctatcgc atcgagctgc aggcttgcaa ccaggacacc cctgaggaac 2581 ggtgcagtgt ggcagcctac gtcagtgcga ggaccatgcc tgaagccaag gctgatgaca 2641 ttgttggccc tgtgacgcat gaaatctttg agaacaacgt cgtccacttg atgtggcagg 2701 agccgaagga gcccaatggt ctgatcgtgc tgtatgaagt gagttatcgg cgatatggtg 2761 atgaggagct gcatctctgc gtctcccgca agcacttcgc tctggaacgg ggctgcaggc 2821 tgcgtgggct gtcaccgggg aactacagcg tgcgaatccg ggccacctcc cttgcgggca 2881 acggctcttg gacggaaccc acctatttct acgtgacaga ctatttagac gtcccgtcaa 2941 atattgcaaa aattatcatc ggccccctca tctttgtctt tctcttcagt gttgtgattg 3001 gaagtattta tctattcctg agaaagaggc agccagatgg gccgctggga ccgctttacg 3061 cttcttcaaa ccctgagtat ctcagtgcca gtgatgtgtt tccatgctct gtgtacgtgc 3121 cggacgagtg ggaggtgtct cgagagaaga tcaccctcct tcgagagctg gggcagggct 3181 ccttcggcat ggtgtatgag ggcaatgcca gggacatcat caagggtgag gcagagaccc 3241 gcgtggcggt gaagacggtc aacgagtcag ccagtctccg agagcggatt gagttcctca 3301 atgaggcctc ggtcatgaag ggcttcacct gccatcacgt ggtgcgcctc ctgggagtgg 3361 tgtccaaggg ccagcccacg ctggtggtga tggagctgat ggctcacgga gacctgaaga 3421 gctacctccg ttctctgcgg ccagaggctg agaataatcc tggccgccct ccccctaccc 3481 ttcaagagat gattcagatg gcggcagaga ttgctgacgg gatggcctac ctgaacgcca 3541 agaagtttgt gcatcgggac ctggcagcga gaaactgcat ggtcgcccat gattttactg 3601 tcaaaattgg agactttgga atgaccagag acatctatga aacggattac taccggaaag 3661 ggggcaaggg tctgctccct gtacggtgga tggcaccgga gtccctgaag gatggggtct 3721 tcaccacttc ttctgacatg tggtcctttg gcgtggtcct ttgggaaatc accagcttgg 3781 cagaacagcc ttaccaaggc ctgtctaatg aacaggtgtt gaaatttgtc atggatggag 3841 ggtatctgga tcaacccgac aactgtccag agagagtcac tgacctcatg cGCATGTGCT 3901 GGCAATTCAA CCCCAAGATG AGGCCAACCT TCCTGGAGAT TGTCAACCTG CTCAAGGACG 3961 ACCTGCACCC CAGCTTTCCA GAGGTGTCGT TCTTCCACAG CGAGGAGAAC AAGGCTCCCG 4021 AGAGTGAGGA GCTGGAGATG GAGTTTGAGG ACATGGAGAA TGTGCCCCTG GACCGTTCCT 4081 CGCACTGTCA GAGGGAGGAG GCGGGGGGCC GGGATGGAGG GTCCTCGCTG GGTTTCAAGC 4141 GGAGCTACGA GGAACACATC CCTTACACAC ACATGAACGG AGGCAAGAAA AACGGGCGGA 4201 TTCTGACCTT GCCTCGGTCC AATCCTTCCT AAAAGGGTGG GCGCGCCGAC CCAGCTTTCT 4261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 4321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 4381 GTGGAAAGGA CGAACTATAT TCATACGTAC TTCCCGAACG CGTTAAGTCg acaatcaacc 4441 tctggattac aaaatttgtg aaagatt