Construct: ORF TRCN0000491418
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020469.2_s317c1
- DNA Barcode:
- CGTGTGCGTCACAACTAAGAGGCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKD3 (23683)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491418
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23683 | PRKD3 | protein kinase D3 | NM_005813.5 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 23683 | PRKD3 | protein kinase D3 | XM_005264237.4 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 23683 | PRKD3 | protein kinase D3 | XM_024452776.1 | 99.8% | 99.8% | (many diffs) |
| 4 | human | 23683 | PRKD3 | protein kinase D3 | XM_005264238.3 | 88.1% | 88.2% | (many diffs) |
| 5 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003741.2 | 80.4% | 80.4% | (many diffs) |
| 6 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003742.2 | 80.4% | 80.4% | (many diffs) |
| 7 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003743.2 | 70.7% | 70.7% | (many diffs) |
| 8 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003744.2 | 70.7% | 70.7% | (many diffs) |
| 9 | human | 23683 | PRKD3 | protein kinase D3 | XM_024452777.1 | 70.7% | 70.7% | (many diffs) |
| 10 | mouse | 75292 | Prkd3 | protein kinase D3 | NM_001171004.1 | 89.6% | 95.8% | (many diffs) |
| 11 | mouse | 75292 | Prkd3 | protein kinase D3 | NM_029239.3 | 89.5% | 95.6% | (many diffs) |
| 12 | mouse | 75292 | Prkd3 | protein kinase D3 | NM_001171005.1 | 80.1% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2742
- ORF length:
- 2670
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctgca aataattccc ctccatcagc ccagaagtct gtattaccca 121 cagctattcc tgctgtgctt ccagctgctt ctccgtgttc aagtcctaag acgggactct 181 ctgcccgact ctctaatgga agcttcagtg caccatcact caccaactcc agaggctcag 241 tgcatacagt ttcatttcta ctgcaaattg gcctcacacg ggagagtgtt accattgaag 301 cccaggaact gtctttatct gctgtcaagg atcttgtgtg ctccatagtt tatcaaaagt 361 ttccagagtg tggattcttt ggcatgtatg acaaaattct tctctttcgc catgacatga 421 actcagaaaa cattttgcag ctgattacct cagcagatga aatacatgaa ggagacctag 481 tggaagtggt tctttcagct ttagccacag tagaagactt ccagattcgt ccacatactc 541 tctatgtaca ttcttacaaa gctcctactt tctgtgatta ctgtggtgag atgctctggg 601 gattggtacg tcaaggactg aaatgtgaag gctgtggatt aaattaccat aaacgatgtg 661 ccttcaagat tccaaataac tgtagtggag taagaaagag acgtctgtca aatgtatctt 721 taccaggacc cggcctctca gttccaagac ccctacagcc tgaatatgta gcccttccca 781 gtgaagagtc acatgtccac caggaaccaa gtaagagaat tccttcttgg agtggtcgcc 841 caatctggat ggaaaagatg gtaatgtgca gagtgaaagt tccacacaca tttgctgttc 901 actcttacac ccgtcccacg atatgtcagt actgcaagcg gttactgaaa ggcctctttc 961 gccaaggaat gcagtgtaaa gattgcaaat tcaactgcca taaatgctgt gcatcaaaag 1021 taccaagaga ctgccttgga gaggttactt tcaatggaga accttccagt ctgggaacag 1081 atacagatat accaatggat atcgacaata atgacataaa tagtgatagt agtcggggtt 1141 tggatgacac agaagagcca tcacccccag aagataagat gttcttcttg gacccatctg 1201 atctcgatgt ggaaagagat gaagaagccg ttaaaacaat cagtccatca acaagcaata 1261 atattccgct catgagggtt gtacaatcca tcaagcacac aaagaggaag agcagcacaa 1321 tggtgaagga agggtggatg gtccattaca ccagcaggga taacctgaga aagaggcatt 1381 attggagact tgacagcaaa tgtctaacat tatttcagaa tgaatctgga tcaaagtatt 1441 ataaggaaat tccactttca gaaattctcc gcatatcttc accacgagat ttcacaaaca 1501 tttcacaagg cagcaatcca cactgttttg aaatcattac tgatactatg gtatacttcg 1561 ttggtgagaa caatggggac agctctcata atcctgttct tgctgccact ggagttggac 1621 ttgatgtagc acagagctgg gaaaaagcaa ttcgccaagc cctcatgcct gttactcctc 1681 aagcaagtgt ttgcacttct ccagggcaag ggaaagatca caaagatttg tctacaagta 1741 tctctgtatc taattgtcag attcaggaga atgtggatat cagtactgtt taccagatct 1801 ttgcagatga ggtgcttggt tcaggccagt ttggcatcgt ttatggagga aaacatagaa 1861 agactgggag ggatgtggct attaaagtaa ttgataagat gagattcccc acaaaacaag 1921 aaagtcaact ccgtaatgaa gtggctattt tacagaattt gcaccatcct gggattgtaa 1981 acctggaatg tatgtttgaa accccagaac gagtctttgt agtaatggaa aagctgcatg 2041 gagatatgtt ggaaatgatt ctatccagtg agaaaagtcg gcttccagaa cgaattacta 2101 aattcatggt cacacagata cttgttgctt tgaggaatct gcattttaag aatattgtgc 2161 actgtgattt aaagccagaa aatgtgctgc ttgcatcagc agagccattt cctcaggtga 2221 agctgtgtga ctttggattt gcacgcatca ttggtgaaaa gtcattcagg agatctgtgg 2281 taggaactcc agcatactta gcccctgaag ttctccggag caaaggttac aaccgttccc 2341 tAGATATGTG GTCAGTGGGA GTTATCATCT ATGTGAGCCT CAGTGGCACA TTTCCTTTTA 2401 ATGAGGATGA AGATATAAAT GACCAAATCC AAAATGCTGC ATTTATGTAC CCACCAAATC 2461 CATGGAGAGA AATTTCTGGT GAAGCAATTG ATCTGATAAA CAATCTGCTT CAAGTGAAGA 2521 TGAGAAAACG TTACAGTGTT GACAAATCTC TTAGTCATCC CTGGCTACAG GACTATCAGA 2581 CTTGGCTTGA CCTTAGAGAA TTTGAAACTC GCATTGGAGA ACGTTACATT ACACATGAAA 2641 GTGATGATGC TCGCTGGGAA ATACATGCAT ACACACATAA CCTTGTATAC CCAAAGCACT 2701 TCATTATGGC TCCTAATCCA GATGATATGG AAGAAGATCC TTAGGACCCA GCTTTCTTGT 2761 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2821 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2881 GAAAGGACGA CGTGTGCGTC ACAACTAAGA GGCTACGCGT TAAGTCgaca atcaacctct 2941 ggattacaaa atttgtgaaa gatt