Construct: ORF TRCN0000491430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020444.2_s317c1
- DNA Barcode:
- TCATCCCTGACAGACCTTTTAGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MOK (5891)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5891 | MOK | MOK protein kinase | NM_001272011.2 | 93% | 92.8% | 603_683del |
| 2 | human | 5891 | MOK | MOK protein kinase | NM_001353827.2 | 92.8% | 92.5% | 319_320insAGC;600_680del |
| 3 | human | 5891 | MOK | MOK protein kinase | NM_014226.3 | 86.3% | 86.1% | 123_212del;693_773del |
| 4 | human | 5891 | MOK | MOK protein kinase | NM_001330234.2 | 86.1% | 85.9% | 123_212del;409_410insAGC;690_770del |
| 5 | human | 5891 | MOK | MOK protein kinase | NM_001353828.2 | 84% | 82.5% | (many diffs) |
| 6 | human | 5891 | MOK | MOK protein kinase | XM_024449681.1 | 83.9% | 82.7% | (many diffs) |
| 7 | human | 5891 | MOK | MOK protein kinase | XM_017021552.1 | 78.5% | 76.3% | (many diffs) |
| 8 | human | 5891 | MOK | MOK protein kinase | NM_001353829.2 | 73.7% | 73.5% | 0_1ins225;378_458del |
| 9 | human | 5891 | MOK | MOK protein kinase | NM_001353830.2 | 73.5% | 73.2% | 0_1ins225;94_95insAGC;375_455del |
| 10 | human | 5891 | MOK | MOK protein kinase | XM_011537052.2 | 69.1% | 66.2% | (many diffs) |
| 11 | human | 5891 | MOK | MOK protein kinase | XM_017021557.2 | 60.4% | 59.3% | (many diffs) |
| 12 | human | 5891 | MOK | MOK protein kinase | XM_017021558.1 | 60.1% | 59.1% | (many diffs) |
| 13 | human | 5891 | MOK | MOK protein kinase | XR_429322.3 | 57% | (many diffs) | |
| 14 | human | 5891 | MOK | MOK protein kinase | XM_017021556.2 | 56.6% | 53.2% | (many diffs) |
| 15 | human | 5891 | MOK | MOK protein kinase | XM_006720225.3 | 56% | 55.1% | (many diffs) |
| 16 | human | 5891 | MOK | MOK protein kinase | XM_006720226.3 | 56% | 55.1% | (many diffs) |
| 17 | human | 5891 | MOK | MOK protein kinase | XM_017021555.2 | 55.8% | 54.8% | (many diffs) |
| 18 | human | 5891 | MOK | MOK protein kinase | NM_001353831.2 | 53.9% | 53.7% | 0_1ins456;147_227del |
| 19 | human | 5891 | MOK | MOK protein kinase | NR_148552.2 | 53.3% | (many diffs) | |
| 20 | human | 5891 | MOK | MOK protein kinase | XM_011537053.3 | 51.8% | 43.4% | (many diffs) |
| 21 | human | 5891 | MOK | MOK protein kinase | XR_943504.2 | 51.4% | (many diffs) | |
| 22 | human | 5891 | MOK | MOK protein kinase | XR_429323.3 | 50.8% | (many diffs) | |
| 23 | human | 5891 | MOK | MOK protein kinase | XR_001750492.2 | 50.6% | (many diffs) | |
| 24 | human | 5891 | MOK | MOK protein kinase | XR_429320.3 | 49.8% | (many diffs) | |
| 25 | human | 5891 | MOK | MOK protein kinase | NM_001353832.2 | 47.5% | 43.2% | (many diffs) |
| 26 | human | 5891 | MOK | MOK protein kinase | XM_024449682.1 | 47.5% | 43.2% | (many diffs) |
| 27 | human | 5891 | MOK | MOK protein kinase | NR_073541.2 | 38.1% | (many diffs) | |
| 28 | human | 5891 | MOK | MOK protein kinase | NR_073543.2 | 37.7% | (many diffs) | |
| 29 | human | 5891 | MOK | MOK protein kinase | NR_073542.2 | 36.9% | (many diffs) | |
| 30 | human | 5891 | MOK | MOK protein kinase | NR_148553.2 | 34.7% | (many diffs) | |
| 31 | human | 5891 | MOK | MOK protein kinase | NR_148555.2 | 32.1% | (many diffs) | |
| 32 | mouse | 26448 | Mok | MOK protein kinase | XM_006515894.3 | 46.1% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1158
- ORF length:
- 1086
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagaac tataaagcaa ttggcaaaat aggagaggga acgttttctg 121 aagttatgaa gatgcaaagc ctgagagatg gaaactacta tgcatgtaaa caaatgaagc 181 agcgctttga aagtgacaga aaatctggtt ctcttgcact aatatgtgaa cttatggaca 241 tgaatattta tgagctaata cgagggagaa gatacccatt atcagaaaaa aaaattatgc 301 actatatgta ccagttatgt aagtccctgg atcatattca cagaaatgga atatttcaca 361 gagatgtaaa accagaaaat atactaataa agcaggatgt cctgaaatta ggggactttg 421 gctcctgccg gagtgtctat tccaagcagc cgtacacgga atacatctcc acccgctggt 481 accgggcccc ggagtgtctc ctcactgatg ggttctacac gtacaagatg gacctgtgga 541 gcgccggctg tgtgttctac gagatcgcca gtctgcagcc cctctttcct ggagtaaatg 601 aactggacca aatctcaaaa atccacgatg tcatcggcac acccgctcag aagatcctca 661 ccaagttcaa acacctctcc ctcctgcacg caatggtggc ctatgatccc gatgagagaa 721 tcgccgccca ccaggccctg cagcacccct acttccaaga acagaggaaa acagagaagc 781 gggctctggg cagccacaga aaagctggct ttccggagca ccctgtggca ccggaaccac 841 tcagtaacag ctgccagatt tccaaggagg gcagaaagca gaaacagtcc cTAAAGCAAG 901 AGGAGGACCG TCCCAAGAGA CGAGGACCGG CCTATGTCAT GGAACTGCCC AAACTAAAGC 961 TTTCGGGAGT GGTCAGACTG TCGTCTTACT CCAGCCCCAC GCTGCAGTCC GTGCTTGGAT 1021 CTGGAACAAA TGGAAGAGTG CCGGTGCTGA GACCCTTGAA GTGCATCCCT GCGAGCAAGA 1081 AGACAGATCC GCAGAAGGAC CTTAAGCCTG CCCCGCAGCA GTGTCGCCTG CCCACCATAG 1141 TGCGGAAAGG CGGAAGATAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAT CCCTGACAGA 1321 CCTTTTAGAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt