Construct: ORF TRCN0000491434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021678.2_s317c1
- DNA Barcode:
- ACAATTTGACCGCTCTACCGATCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TNFSF14 (8740)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491434
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8740 | TNFSF14 | TNF superfamily member 14 | NM_003807.4 | 99.8% | 99.5% | 640A>G |
| 2 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XM_017027417.1 | 99.8% | 99.5% | 640A>G |
| 3 | human | 8740 | TNFSF14 | TNF superfamily member 14 | NM_172014.3 | 84.8% | 84.5% | 111_112ins108;532A>G |
| 4 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XM_017027418.1 | 48.1% | 45.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 789
- ORF length:
- 720
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaggagagt gtcgtacggc cctcagtgtt tgtggtggat ggacagaccg 121 acatcccatt cacgaggctg ggacgaagcc accggagaca gtcgtgcagt gtggcccggg 181 tgggtctggg tctcttgctg ttgctgatgg gggccgggct ggccgtccaa ggctggttcc 241 tcctgcagct gcactggcgt ctaggagaga tggtcacccg cctgcctgac ggacctgcag 301 gctcctggga gcagctgata caagagcgaa ggtctcacga ggtcaaccca gcagcgcatc 361 tcacaggggc caactccagc ttgaccggca gcggggggcc gctgttatgg gagactcagc 421 tgggcctggc cttcctgagg ggcctcagct accacgatgg ggcccttgtg gtcaccaaag 481 ctggctacta ctacatctac TCCAAGGTGC AGCTGGGCGG TGTGGGCTGC CCGCTGGGCC 541 TGGCCAGCAC CATCACCCAC GGCCTCTACA AGCGCACACC CCGCTACCCC GAGGAGCTGG 601 AGCTGTTGGT CAGCCAGCAG TCACCCTGCG GACGGGCCAC CAGCAGCTCC CGGGTCTGGT 661 GGGACAGCAG CTTCCTGGGT GGTGTGGTAC ACCTGGAGGC TGGGGAGGAG GTGGTCGTCC 721 GTGTGCTGGA TGAACGCCTG GTTCGACTGC GTGATGGTAC CCGGTCTTAC TTCGGGGCTT 781 TCATGGTGTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA ATTTGACCGC TCTACCGATC 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t