Construct: ORF TRCN0000491439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020802.2_s317c1
- DNA Barcode:
- CGACCAGAAATTGTACTAACTCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CCR3 (1232)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491439
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | NM_001837.4 | 100% | 100% | |
| 2 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | NM_178329.3 | 100% | 100% | |
| 3 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | XM_006712960.3 | 100% | 100% | |
| 4 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | XM_011533335.2 | 100% | 100% | |
| 5 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | NM_001164680.2 | 95.1% | 95.1% | 1_54del |
| 6 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | NM_178328.1 | 94.4% | 94.4% | 1_63del |
| 7 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | XM_017005685.1 | 86.5% | 86.5% | 1_165del |
| 8 | human | 1232 | CCR3 | C-C motif chemokine receptor 3 | XM_017005686.1 | 86.5% | 86.5% | 1_165del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1137
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacaacc tcactagata cagttgagac ctttggtacc acatcctact 121 atgatgacgt gggcctgctc tgtgaaaaag ctgataccag agcactgatg gcccagtttg 181 tgcccccgct gtactccctg gtgttcactg tgggcctctt gggcaatgtg gtggtggtga 241 tgatcctcat aaaatacagg aggctccgaa ttatgaccaa catctacctg ctcaacctgg 301 ccatttcgga cctgctcttc ctcgtcaccc ttccattctg gatccactat gtcagggggc 361 ataactgggt ttttggccat ggcatgtgta agctcctctc agggttttat cacacaggct 421 tgtacagcga gatctttttc ataatcctgc tgacaatcga caggtacctg gccattgtcc 481 atgctgtgtt tgcccttcga gcccggactg tcacttttgg tgtcatcacc agcatcgtca 541 cctggggcct ggcagtgcta gcagctcttc ctgaatttat cttctatgag actgaagagt 601 tgtttgaaga gactctttgc agtgctcttt acccagagga tacagtatat agctggaggc 661 atttccacac tctgagaatg accatcttct gtctcgttct ccctctgctc gttatggcca 721 tctgctacac aggaatcatc aaaacgctgc tgaggtgccc cagtaaaaaa aagtacaagg 781 CCATCCGGCT CATTTTTGTC ATCATGGCGG TGTTTTTCAT TTTCTGGACA CCCTACAATG 841 TGGCTATCCT TCTCTCTTCC TATCAATCCA TCTTATTTGG AAATGACTGT GAGCGGAGCA 901 AGCATCTGGA CCTGGTCATG CTGGTGACAG AGGTGATCGC CTACTCCCAC TGCTGCATGA 961 ACCCGGTGAT CTACGCCTTT GTTGGAGAGA GGTTCCGGAA GTACCTGCGC CACTTCTTCC 1021 ACAGGCACTT GCTCATGCAC CTGGGCAGAT ACATCCCATT CCTTCCTAGT GAGAAGCTGG 1081 AAAGAACCAG CTCTGTCTCT CCATCCACAG CAGAGCCGGA ACTCTCTATT GTGTTTTAGG 1141 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGACGACC AGAAATTGTA CTAACTCGGA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt