Construct: ORF TRCN0000491441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020681.2_s317c1
- DNA Barcode:
- AATCGGACAGTCCTAAGGGAAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NUAK1 (9891)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9891 | NUAK1 | NUAK family kinase 1 | NM_014840.2 | 100% | 100% | |
| 2 | mouse | 77976 | Nuak1 | NUAK family, SNF1-like kina... | NM_001004363.2 | 85.8% | 90.7% | (many diffs) |
| 3 | mouse | 77976 | Nuak1 | NUAK family, SNF1-like kina... | XM_011243603.3 | 68.7% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2055
- ORF length:
- 1983
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaggg gccgccgcgc ctgtggcggg ggaccgcccc gacttggggc 121 tgggggcgcc gggctctccc cgagaggcgg tggcgggggc gactgcagcc ctggagccca 181 ggaagccgca cggggtgaag cggcatcacc acaagcacaa cttgaagcac cgctacgagc 241 tgcaggagac cctgggcaaa ggcacctacg gcaaagtcaa gcgggccacc gagaggtttt 301 ctggccgagt ggttgctata aaatccattc gtaaggacaa aattaaggat gaacaagaca 361 tggttcacat cagacgagag attgagatca tgtcatctct caaccatcct catatcatca 421 gtatttatga agtgtttgag aacaaagata agattgtgat catcatggaa tatgccagca 481 aaggggagct gtacgattac atcagtgagc ggcgacgcct cagtgagagg gagacccggc 541 acttcttccg gcagatcgtc tctgctgtgc actattgtca caagaacggt gtggtccacc 601 gggacttgaa gctggaaaat atactgctcg atgacaactg caatattaag attgctgact 661 ttgggctttc caacctgtac cagaaggata agttcttaca aacgttttgt gggagtccac 721 tctatgcatc tcctgagatt gtcaatggga gaccttaccg agggccagag gtggacagct 781 gggccctggg tgtgttgctt tacactcttg tttatggaac aatgcccttc gatggtttcg 841 atcacaaaaa cctcattcgg caaatcagca gcggagagta ccgggagcca acacagccct 901 cagatgctcg aggactcata cggtggatgc tgatggtgaa ccccgatcgc cgggccacta 961 ttgaggacat tgccaaccac tggtgggtga actggggcta taagagcagc gtgtgtgact 1021 gtgatgccct ccatgactct gagtccccac tcctggctcg gatcattgac tggcaccacc 1081 gttccacagg gctgcaggct gacaccgaag ccaaaatgaa gggcctggcc aaacccacga 1141 cctctgaggt catgctagag cggcagcggt cgctgaagaa atccaagaaa gagaatgact 1201 ttgctcagtc tggtcaggat gcagtgcctg aaagcccatc caagttgagt tctaagaggc 1261 ccaaggggat cctgaagaag cgaagcaaca gcgagcatcg ctctcacagc actggcttca 1321 ttgaaggtgt agttggtcct gccttaccct ctactttcaa gatggagcag gacttgtgca 1381 ggactggcgt gctcctccca agctcaccag aggcagaggt gccgggaaaa ctcagcccca 1441 agcagtcggc cacgatgccc aagaaaggca tcttgaaaaa gacccagcag agagaatcag 1501 gttactactc ttccccagag cgcagtgagt cttcggagct gttggacagt aatgatgtga 1561 tgggcagcag catcccctcc cccagccccc cggacccagc cagggtaacc tcccacagcc 1621 tctcctgccg gaggaagggc atcttgaaac acagcagcaa atactcagcg ggcaccatgg 1681 acccagccct ggtcagcccT GAAATGCCCA CACTGGAATC CCTGTCAGAG CCTGGTGTCC 1741 CTGCCGAGGG CCTCTCCCGG AGCTACAGCC GCCCTTCCAG TGTCATCAGC GATGACAGCG 1801 TGCTGTCCAG CGACTCTTTT GACTTGCTGG ATTTGCAGGA GAATCGCCCT GCCCGCCAGC 1861 GCATCCGCAG CTGCGTCTCT GCAGAAAACT TCCTCCAGAT CCAGGACTTT GAGGGGCTCC 1921 AGAACCGGCC CCGGCCCCAG TACCTGAAGC GGTACCGGAA CCGGCTGGCA GACAGCAGCT 1981 TCTCCCTCCT CACAGACATG GATGATGTGA CTCAGGTCTA CAAGCAAGCG CTGGAGATCT 2041 GCAGCAAGCT CAACTGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 2101 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 2161 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATCGGAC AGTCCTAAGG 2221 GAAGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt