Transcript: Mouse NM_001004363.2

Mus musculus NUAK family, SNF1-like kinase, 1 (Nuak1), mRNA.

Source:
NCBI, updated 2019-03-12
Taxon:
Mus musculus (mouse)
Gene:
Nuak1 (77976)
Length:
5634
CDS:
312..2288

Additional Resources:

NCBI RefSeq record:
NM_001004363.2
NBCI Gene record:
Nuak1 (77976)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001004363.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360830 CCGGAGGAAGGGCATCTTAAA pLKO_005 1871 CDS 100% 13.200 18.480 N Nuak1 n/a
2 TRCN0000221206 CCGTCCAGTATCATCAGTGAT pLKO.1 2016 CDS 100% 4.950 6.930 N Nuak1 n/a
3 TRCN0000221207 CGAGCTAGACATGGTTCACAT pLKO.1 593 CDS 100% 4.950 6.930 N Nuak1 n/a
4 TRCN0000350746 AGGCCGAGTGGTTGCTATAAA pLKO_005 545 CDS 100% 15.000 10.500 N Nuak1 n/a
5 TRCN0000360831 ACGATGTGACTCAGGTGTATA pLKO_005 2233 CDS 100% 13.200 9.240 N Nuak1 n/a
6 TRCN0000321667 TTGATGACAACTGCAACATTA pLKO_005 871 CDS 100% 13.200 9.240 N Nuak1 n/a
7 TRCN0000321666 GATTGTGTCTGCCGTGCATTA pLKO_005 797 CDS 100% 10.800 7.560 N Nuak1 n/a
8 TRCN0000284951 TTCACATCAGACGAGAGATTG pLKO_005 607 CDS 100% 10.800 7.560 N NUAK1 n/a
9 TRCN0000000903 ACCATCCTCATATCATCAGTA pLKO.1 646 CDS 100% 4.950 3.465 N NUAK1 n/a
10 TRCN0000221209 GCTTGATGACAACTGCAACAT pLKO.1 869 CDS 100% 4.950 3.465 N Nuak1 n/a
11 TRCN0000221205 CCATCCTCATATCATCAGTAT pLKO.1 647 CDS 100% 4.950 2.970 N Nuak1 n/a
12 TRCN0000221208 GCAGAGAGAATCTGGCTACTA pLKO.1 1730 CDS 100% 4.950 2.970 N Nuak1 n/a
13 TRCN0000321730 GCAGAGAGAATCTGGCTACTA pLKO_005 1730 CDS 100% 4.950 2.970 N Nuak1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001004363.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491441 AATCGGACAGTCCTAAGGGAAGTC pLX_317 18.2% 85.8% 90.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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