Construct: ORF TRCN0000491446

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000717.2_s317c1
Derived from:
ccsbBroadEn_08694
DNA Barcode:
ACCTAACCTAACTACGCTTGAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TBX20 (57057)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491446
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57057 TBX20 T-box transcription factor 20 NM_001166220.1 99.8% 100% 39T>C
2 human 57057 TBX20 T-box transcription factor 20 NM_001077653.2 66.3% 66.4% 39T>C;892_1341del
3 human 57057 TBX20 T-box transcription factor 20 XM_017012456.1 21.9% 21.9% 0_1ins597;295_744del
4 mouse 57246 Tbx20 T-box 20 NM_020496.3 89.2% 97.6% (many diffs)
5 mouse 57246 Tbx20 T-box 20 NM_001205085.1 88% 96.3% (many diffs)
6 mouse 57246 Tbx20 T-box 20 NM_194263.2 59.6% 65.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
960
ORF length:
891
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagttcacg gcgtccccca agccccaact ctcctcccgg gccaacgcct
121tctccattgc cgcgctcatg tcgagcggcg gctctaagga gaaggaggcg acggagaaca
181caatcaaacc cctggagcaa tttgtggaga agtcgtcctg tgcccagccc ctgggtgagc
241tgaccagcct ggatgctcat ggggagtttg gtggaggcag tggcagcagc ccgtcctcct
301cctctctgtg cactgagcca ctgatcccca ccacccccat catccccagt gaggaaatgg
361ccaaaattgc ctgcagcctg gagaccaagg agctttggga caaattccat gagctgggca
421ccgagatgat catcaccaag tcgggcagga ggatgtttcc aaccatccgg gtgtcctttt
481cgggggtgga tcctgaggcc aagtacatag tcctgatgga catcgtccct gtggacaaca
541agaggtaccg ctacgcctac caccggtccT CCTGGCTGGT GGCTGGCAAG GCCGACCCGC
601CGTTGCCAGC CAGGCTCTAT GTGCATCCAG ATTCTCCTTT TACCGGTGAG CAACTACTCA
661AACAGATGGT GTCTTTTGAA AAGGTGAAAC TCACCAACAA TGAACTGGAT CAACATGGCC
721ATATAATTTT GAACTCAATG CATAAGTACC AGCCAAGGGT GCACATCATT AAGAAGAAAG
781ACCACACAGC CTCATTGCTC AACCTGAAGT CTGAAGAATT TAGAACTTTC ATCTTTCCAG
841AAACAGTTTT TACGGCAGTC ACTGCCTACC AGAATCAACT GATAACGAAG CTGAAAATAG
901ATAGCAATCC TTTTGCCAAA GGATTCCGGG ATTCCTCCAG GCTCACTGAC ATTGAGAGGT
961TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1021GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1081TTTATATATC TTGTGGAAAG GACGAACCTA ACCTAACTAC GCTTGAACGA CGCGTTAAGT
1141Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)