Construct: ORF TRCN0000491446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000717.2_s317c1
- Derived from:
- ccsbBroadEn_08694
- DNA Barcode:
- ACCTAACCTAACTACGCTTGAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBX20 (57057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57057 | TBX20 | T-box transcription factor 20 | NM_001166220.1 | 99.8% | 100% | 39T>C |
| 2 | human | 57057 | TBX20 | T-box transcription factor 20 | NM_001077653.2 | 66.3% | 66.4% | 39T>C;892_1341del |
| 3 | human | 57057 | TBX20 | T-box transcription factor 20 | XM_017012456.1 | 21.9% | 21.9% | 0_1ins597;295_744del |
| 4 | mouse | 57246 | Tbx20 | T-box 20 | NM_020496.3 | 89.2% | 97.6% | (many diffs) |
| 5 | mouse | 57246 | Tbx20 | T-box 20 | NM_001205085.1 | 88% | 96.3% | (many diffs) |
| 6 | mouse | 57246 | Tbx20 | T-box 20 | NM_194263.2 | 59.6% | 65.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 960
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagttcacg gcgtccccca agccccaact ctcctcccgg gccaacgcct 121 tctccattgc cgcgctcatg tcgagcggcg gctctaagga gaaggaggcg acggagaaca 181 caatcaaacc cctggagcaa tttgtggaga agtcgtcctg tgcccagccc ctgggtgagc 241 tgaccagcct ggatgctcat ggggagtttg gtggaggcag tggcagcagc ccgtcctcct 301 cctctctgtg cactgagcca ctgatcccca ccacccccat catccccagt gaggaaatgg 361 ccaaaattgc ctgcagcctg gagaccaagg agctttggga caaattccat gagctgggca 421 ccgagatgat catcaccaag tcgggcagga ggatgtttcc aaccatccgg gtgtcctttt 481 cgggggtgga tcctgaggcc aagtacatag tcctgatgga catcgtccct gtggacaaca 541 agaggtaccg ctacgcctac caccggtccT CCTGGCTGGT GGCTGGCAAG GCCGACCCGC 601 CGTTGCCAGC CAGGCTCTAT GTGCATCCAG ATTCTCCTTT TACCGGTGAG CAACTACTCA 661 AACAGATGGT GTCTTTTGAA AAGGTGAAAC TCACCAACAA TGAACTGGAT CAACATGGCC 721 ATATAATTTT GAACTCAATG CATAAGTACC AGCCAAGGGT GCACATCATT AAGAAGAAAG 781 ACCACACAGC CTCATTGCTC AACCTGAAGT CTGAAGAATT TAGAACTTTC ATCTTTCCAG 841 AAACAGTTTT TACGGCAGTC ACTGCCTACC AGAATCAACT GATAACGAAG CTGAAAATAG 901 ATAGCAATCC TTTTGCCAAA GGATTCCGGG ATTCCTCCAG GCTCACTGAC ATTGAGAGGT 961 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAACCTA ACCTAACTAC GCTTGAACGA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt