Construct: ORF TRCN0000491450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021507.2_s317c1
- DNA Barcode:
- TTGCACAGCAGGTGCTAGAGGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EYA2 (2139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491450
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2139 | EYA2 | EYA transcriptional coactiv... | NM_005244.5 | 95.4% | 95.3% | 294_365del;1082G>C |
| 2 | human | 2139 | EYA2 | EYA transcriptional coactiv... | XM_017027721.2 | 89.9% | 89.7% | 294_365del;886_887ins90;992G>C |
| 3 | human | 2139 | EYA2 | EYA transcriptional coactiv... | XM_005260327.2 | 86.6% | 80.1% | (many diffs) |
| 4 | human | 2139 | EYA2 | EYA transcriptional coactiv... | NM_172110.3 | 80.7% | 80.6% | 294_365del;1082G>C;1197_1198ins237 |
| 5 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | NM_001271962.1 | 79.1% | 83.8% | (many diffs) |
| 6 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | NM_001271963.1 | 79.1% | 83.8% | (many diffs) |
| 7 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | NM_010165.3 | 79.1% | 83.8% | (many diffs) |
| 8 | mouse | 14049 | Eya2 | EYA transcriptional coactiv... | XM_006498739.3 | 71.3% | 77.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1611
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtagaacta gtgatctcac ccagcctcac tgtaaacagc gattgtctgg 121 ataaactgaa gtttaaccgt gctgacgctg ctgtgtggac tctgagtgac agacaaggca 181 tcaccaaatc ggcccccctg agagtgtccc agctcttctc cagatcttgc ccacgtgtcc 241 tcccccgcca gccttccaca gccatggcag cctacggcca gacgcagtac agtgcgggga 301 tccagcaggc taccccctat acagcttacc cacctccagc acaagcctat ggaatccctt 361 ccttcagcac ctcacccact ggacagagcc catacaccta ccagatgcac ggcacaacag 421 ggttctatca aggaggaaat ggactgggca acgcagccgg tttcgggagt gtgcaccagg 481 actatccttc ctaccccggc ttcccccaga gccagtaccc ccagtattac ggctcatcct 541 acaaccctcc ctacgtcccg gccagcagca tctgcccttc gcccctctcc acgtccacct 601 acgtcctcca ggaggcatct cacaacgtcc ccaaccagag ttccgagtca cttgctggtg 661 aatacaacac acacaatgga ccttccacac cagcgaaaga gggagacaca gacaggccgc 721 accgggcctc cgacgggaag ctccgaggcc ggtctaagag gagcagtgac ccgtccccgg 781 caggggacaa tgagattgag cgtgtgttcg tgtgggactt ggatgagaca ataattattt 841 ttcactcctt actcacgggg acatttgcat ccagatacgg gaaggacacc acgacgtccg 901 tgcgcattgg ccttatgatg gaagagatga tcttcaacct tgcagataca catctgttct 961 tcaatgacct ggaggattgt gaccagatcc acgttgatga cgtctcatca gatgacaatg 1021 gccaagattt aagcacatac aacttctccg ctgacggctt ccacagttcg gccccagcag 1081 ccaacctgtg cctgggctct ggcgtgcacg gcggcgtgga ctggatgagg aagctggcct 1141 tccgctaccg gcgggtgaag gagatgtaca atacctacaa gaacaacgtt ggtgggttga 1201 taggcactcc caaaagggag acctggctac agctccgagc tgagctggaa gctctcacag 1261 acctctggct gacccactcc ctgaaggcac taaaccTCAT CAACTCCCGG CCCAACTGTG 1321 TCAATGTGCT GGTCACCACC ACTCAACTAA TTCCTGCCCT GGCCAAAGTC CTGCTATATG 1381 GCCTGGGGTC TGTGTTTCCT ATTGAGAACA TCTACAGTGC AACCAAGACA GGGAAGGAGA 1441 GCTGCTTCGA GAGGATAATG CAGAGATTCG GCAGAAAAGC TGTCTACGTG GTGATCGGTG 1501 ATGGTGTGGA AGAGGAGCAA GGAGCGAAAA AGCACAACAT GCCTTTCTGG CGGATATCCT 1561 GCCACGCAGA CCTGGAGGCA CTGAGGCACG CCCTGGAGCT GGAGTATTTA TAGGACCCAG 1621 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1681 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1741 TATCTTGTGG AAAGGACGAT TGCACAGCAG GTGCTAGAGG TTAACGCGTT AAGTCgacaa 1801 tcaacctctg gattacaaaa tttgtgaaag att