Construct: ORF TRCN0000491453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012522.2_s317c1
- Derived from:
- ccsbBroadEn_08921
- DNA Barcode:
- CGCCTTTATCGAGCTGTTGGCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF26 (79102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79102 | RNF26 | ring finger protein 26 | NM_032015.5 | 99.9% | 100% | 543G>A |
| 2 | mouse | 213211 | Rnf26 | ring finger protein 26 | NM_153762.3 | 83.6% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcagtgtac ctggtagtga atgggttggg cctggtgctg gacgtgctga 121 ccttggtgtt ggacctcaac ttcctgctgg tgtcctccct cctggcttcc ctggcctggc 181 tcctggcctt cgtctacaac ctgccgcaca cggtactgac tagtcttctg cacttgggcc 241 gcggagtctt gctttcattg ctggccttga tcgaagccgt ggtccggttc acatgtgggg 301 gcttgcaggc cttgtgtact ctgctgtata gctgctgctc tggcctagag agcctaaagc 361 tcctggggca cctggcctct catggggcac tgcggagcag ggagatactg caccggggcg 421 tcctcaatgt ggtctccagt ggccatgctt tgctgcgcca ggcctgtgac atctgtgcca 481 ttgccatgag cctggtggct tatgtgatca acagcctggt caacatctgc ctcatcggca 541 ctcagaacct cttttccctg gtgctggccc tgtgggatgc agtgaccggg cctctgtgga 601 ggatgacaga cgtagtggct gccttcctag cccacatttc cagcagtgct gtggccatgg 661 ccatcctcct ttggacaccc tgccaactag ccctggagct gctggcctca gctgcccgcc 721 tcctggccag ctttgtgctt gtcaatctca ctggcttggt gttgctagct tgtgtgctgg 781 cagtgacggt gactgtgttg catccggact tcaccctgag gctggctacc caggcactca 841 gccagctcca tgcccggcca tcctaccacc gtcttcgaga ggatgtcatg cggctctctc 901 gcctagcact gggctcagag gcctggcgcc gagtctggag ccgcagtctg cagctggcga 961 gttggccaaa ccggggaggg gcacctggag ctccccaggg tgaccctatg agggtattct 1021 cagttaggac ccggagacag gacactcttc cTGAAGCGGG GCGCAGATCA GAGGCAGAAG 1081 AGGAGGAGGC CAGGACCATC AGAGTGACAC CTGTCAGGGG CCGAGAGAGG CTCAATGAGG 1141 AGGAGCCTCC AGGTGGGCAA GACCCTTGGA AATTGCTGAA GGAGCAAGAG GAGCGGAAGA 1201 AGTGTGTCAT CTGCCAGGAC CAGAGCAAGA CAGTGTTGCT CCTGCCCTGC CGGCATCTGT 1261 GCCTGTGCCA GGCCTGCACT GAAATCCTGA TGCGCCACCC CGTCTACCAC CGCAATTGCC 1321 CGCTCTGCCG CCGGGGCATC CTGCAGACCC TCAATGTCTA CCTCTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA CGCCTTTATC GAGCTGTTGG CGCAACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt