Construct: ORF TRCN0000491456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020674.2_s317c1
- DNA Barcode:
- TTGACCTCCGAGAGACATACCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKCH (5583)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491456
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5583 | PRKCH | protein kinase C eta | NM_006255.4 | 99.9% | 100% | 393T>C;1674C>T |
| 2 | human | 5583 | PRKCH | protein kinase C eta | XM_011536954.3 | 86.3% | 83.7% | (many diffs) |
| 3 | human | 5583 | PRKCH | protein kinase C eta | XM_011536955.1 | 83.2% | 82.1% | (many diffs) |
| 4 | human | 5583 | PRKCH | protein kinase C eta | XM_017021458.1 | 76.3% | 76.4% | 0_1ins483;1191C>T |
| 5 | human | 5583 | PRKCH | protein kinase C eta | XM_024449661.1 | 76.3% | 76.4% | 0_1ins483;1191C>T |
| 6 | human | 5583 | PRKCH | protein kinase C eta | XM_024449662.1 | 76.3% | 76.4% | 0_1ins483;1191C>T |
| 7 | human | 5583 | PRKCH | protein kinase C eta | XM_011536956.1 | 74.4% | 71.7% | (many diffs) |
| 8 | human | 5583 | PRKCH | protein kinase C eta | XM_011536957.1 | 70.6% | 70.1% | (many diffs) |
| 9 | human | 5583 | PRKCH | protein kinase C eta | XM_017021459.1 | 64.1% | 63.2% | (many diffs) |
| 10 | mouse | 18755 | Prkch | protein kinase C, eta | NM_008856.4 | 89.9% | 97.6% | (many diffs) |
| 11 | mouse | 18755 | Prkch | protein kinase C, eta | NM_001313977.1 | 74.5% | 79.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2121
- ORF length:
- 2049
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcgtct ggcaccatga agttcaatgg ctatttgagg gtccgcatcg 121 gtgaggcagt ggggctgcag cccacccgct ggtccctgcg ccactcgctc ttcaagaagg 181 gccaccagct gctggacccc tatctgacgg tgagcgtgga ccaggtgcgc gtgggccaga 241 ccagcaccaa gcagaagacc aacaaaccca cgtacaacga ggagttttgc gctaacgtca 301 ccgacggcgg ccacctcgag ttggccgtct tccacgagac gcccctgggc tacgaccact 361 tcgtggccaa ctgcaccctg cagttccagg agctgctgcg cacgaccggc gcctcggaca 421 ccttcgaggg ttgggtggat ctcgagccag aggggaaagt attcgtggta ataaccctta 481 ccgggagttt cactgaagct actctccaga gagaccggat cttcaaacat tttaccagga 541 agcgccaaag ggctatgcga aggcgagtcc accagatcaa tggacacaag ttcatggcca 601 cgtatctgag gcagcccacc tactgctctc actgcaggga gtttatctgg ggagtgtttg 661 ggaaacaggg ttatcagtgc caagtgtgca cctgtgtcgt ccataaacgc tgccatcatc 721 taattgttac agcctgtact tgccaaaaca atattaacaa agtggattca aagattgcag 781 aacagaggtt cgggatcaac atcccacaca agttcagcat ccacaactac aaagtgccaa 841 cattctgcga tcactgtggc tcactgctct ggggaataat gcgacaagga cttcagtgta 901 aaatatgtaa aatgaatgtg catattcgat gtcaagcgaa cgtggcccct aactgtgggg 961 taaatgcggt ggaacttgcc aagaccctgg cagggatggg tctccaaccc ggaaatattt 1021 ctccaacctc gaaactcgtt tccagatcga ccctaagacg acagggaaag gagagcagca 1081 aagaaggaaa tgggattggg gttaattctt ccaaccgact tggtatcgac aactttgagt 1141 tcatccgagt gttggggaag gggagttttg ggaaggtgat gcttgcaaga gtaaaagaaa 1201 caggagacct ctatgctgtg aaggtgctga agaaggacgt gattctgcag gatgatgatg 1261 tggaatgcac catgaccgag aaaaggatcc tgtctctggc ccgcaatcac cccttcctca 1321 ctcagttgtt ctgctgcttt cagacccccg atcgtctgtt ttttgtgatg gagtttgtga 1381 atgggggtga cttgatgttc cacattcaga agtctcgtcg ttttgatgaa gcacgagctc 1441 gcttctatgc tgcagaaatc atttcggctc tcatgttcct ccatgataaa ggaatcatct 1501 atagagatct gaaactggac aatgtcctgt tggaccacga gggtcactgt aaactggcag 1561 acttcggaat gtgcaaggag gggatttgca atggtgtcac cacggccaca ttctgtggca 1621 cgccagacta tatcgctcca gagatcctcc aggaaatgct gtacgggcct gcagtagact 1681 ggtgggcaat gggcgtgttg ctctatgaga tgctctgtgg tcacgcgcct tttgaggcag 1741 agaatgaaga tgacctcttt gaggccatac tgaatgatga ggtggtctac cctacctggc 1801 tccatgaaga tgccaCAGGG ATCCTAAAAT CTTTCATGAC CAAGAACCCC ACCATGCGCT 1861 TGGGCAGCCT GACTCAGGGA GGCGAGCACG CCATCTTGAG ACATCCTTTT TTTAAGGAAA 1921 TCGACTGGGC CCAGCTGAAC CATCGCCAAA TAGAACCGCC TTTCAGACCC AGAATCAAAT 1981 CCCGAGAAGA TGTCAGTAAT TTTGACCCTG ACTTCATAAA GGAAGAGCCA GTTTTAACTC 2041 CAATTGATGA GGGACATCTT CCAATGATTA ACCAGGATGA GTTTAGAAAC TTTTCCTATG 2101 TGTCTCCAGA ATTGCAACCA TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2161 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2221 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGACCTCCGA 2281 GAGACATACC CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2341 att