Transcript: Human XM_017021459.1

PREDICTED: Homo sapiens protein kinase C eta (PRKCH), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRKCH (5583)
Length:
1891
CDS:
542..1876

Additional Resources:

NCBI RefSeq record:
XM_017021459.1
NBCI Gene record:
PRKCH (5583)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017021459.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361369 GGGTCTCCAACCCGGAAATAT pLKO_005 1468 CDS 100% 15.000 21.000 N Prkch n/a
2 TRCN0000413744 ATAACCCTTACCGGGAGTTTC pLKO_005 941 CDS 100% 10.800 15.120 N PRKCH n/a
3 TRCN0000022811 CGACAAGGACTTCAGTGTAAA pLKO.1 1352 CDS 100% 13.200 9.240 N Prkch n/a
4 TRCN0000413357 TGCTGAAGAAGGACGTGATTC pLKO_005 1695 CDS 100% 10.800 7.560 N PRKCH n/a
5 TRCN0000006295 GCTGCCATCATCTAATTGTTA pLKO.1 1179 CDS 100% 5.625 3.938 N PRKCH n/a
6 TRCN0000195662 CCAAGCAGAAGACCAACAAAC pLKO.1 717 CDS 100% 10.800 6.480 N PRKCH n/a
7 TRCN0000006297 CCACCAGATCAATGGACACAA pLKO.1 1039 CDS 100% 4.950 2.970 N PRKCH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017021459.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14792 pDONR223 0% 64.1% 63.2% None (many diffs) n/a
2 ccsbBroad304_14792 pLX_304 0% 64.1% 63.2% V5 (many diffs) n/a
3 TRCN0000491934 ATCATCAGAGAAGGCTGTCTTTCT pLX_317 9.9% 64.1% 63.2% V5 (many diffs) n/a
4 TRCN0000491456 TTGACCTCCGAGAGACATACCCTT pLX_317 15.3% 64.1% 63.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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