Construct: ORF TRCN0000491458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019191.2_s317c1
- DNA Barcode:
- AGGGCGAGTATAGAGCTCCTGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LYPD1 (116372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491458
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_144586.7 | 100% | 100% | |
| 2 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001321234.2 | 89.1% | 87.2% | (many diffs) |
| 3 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001077427.4 | 63.1% | 63.1% | 0_1ins156 |
| 4 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001321235.2 | 63.1% | 63.1% | 0_1ins156 |
| 5 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | XM_011510546.3 | 53.9% | 53.9% | 0_1ins195 |
| 6 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_145100.4 | 89.5% | 93.6% | (many diffs) |
| 7 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_017312443.1 | 80% | 80.8% | (many diffs) |
| 8 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_001311089.1 | 56% | 58.1% | (many diffs) |
| 9 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_001311090.1 | 56% | 58.1% | (many diffs) |
| 10 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_006529885.3 | 56% | 58.1% | (many diffs) |
| 11 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_011248088.2 | 43.3% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 498
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtgg gtcctaggca tcgcggcaac tttttgcgga ttgttcttgc 121 ttccaggctt TGCGCTGCAA ATCCAGTGCT ACCAGTGTGA AGAATTCCAG CTGAACAACG 181 ACTGCTCCTC CCCCGAGTTC ATTGTGAATT GCACGGTGAA CGTTCAAGAC ATGTGTCAGA 241 AAGAAGTGAT GGAGCAAAGT GCCGGGATCA TGTACCGCAA GTCCTGTGCA TCATCAGCGG 301 CCTGTCTCAT CGCCTCTGCC GGGTACCAGT CCTTCTGCTC CCCAGGGAAA CTGAACTCAG 361 TTTGCATCAG CTGCTGCAAC ACCCCTCTTT GTAACGGGCC AAGGCCCAAG AAAAGGGGAA 421 GTTCTGCCTC GGCCCTCAGG CCAGGGCTCC GCACCACCAT CCTGTTCCTC AAATTAGCCC 481 TCTTCTCGGC ACACTGCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 541 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 601 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGGGCGA GTATAGAGCT 661 CCTGACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt