Construct: ORF TRCN0000491459
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019939.2_s317c1
- DNA Barcode:
- AAAGGTGTCCGTACCCTGTTCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRE3 (84658)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491459
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84658 | ADGRE3 | adhesion G protein-coupled ... | NM_032571.5 | 99.8% | 99.6% | 1154G>A;1956_1957insG |
| 2 | human | 84658 | ADGRE3 | adhesion G protein-coupled ... | XM_011528374.2 | 97.2% | 96.9% | 25_26ins51;1103G>A;1905_1906insG |
| 3 | human | 84658 | ADGRE3 | adhesion G protein-coupled ... | NM_001289158.1 | 91.9% | 91.7% | 198_199ins156;998G>A;1800_1801insG |
| 4 | human | 84658 | ADGRE3 | adhesion G protein-coupled ... | XR_001753772.1 | 85% | 1_79del;1233G>A;2036_2300delinsG | |
| 5 | human | 84658 | ADGRE3 | adhesion G protein-coupled ... | NM_001289159.2 | 80.5% | 80.3% | 198_199ins378;776G>A;1578_1579insG |
| 6 | human | 84658 | ADGRE3 | adhesion G protein-coupled ... | XM_017027383.1 | 66.9% | 66.7% | 0_1ins645;509G>A;1311_1312insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2031
- ORF length:
- 1959
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcaggga ccattgcttc ttccaggcct ctgctttctg ctgagcctct 121 ttggagctgt gactcagaaa accaaaactt cctgtgctaa gtgcccccca aatgcttcct 181 gtgtcaataa cactcactgc acctgcaacc atggatatac ttctggatct gggcagaaac 241 tattcacatt ccccttggag acatgtaacg acattaatga atgtacacca ccctatagtg 301 tatattgtgg atttaacgct gtgtgttaca atgtcgaagg aagtttctac tgtcaatgtg 361 tcccaggata tagactgcat tctgggaatg aacaattcag taattccaat gagaacacct 421 gtcaggacac cacctcctca aagacaaccg agggcaggaa agagctgcaa aagattgtgg 481 acaaatttga gtcacttctc accaatcaga ctttatggag aacagaaggg agacaagaaa 541 tctcatccac agctaccact attctccggg atgtggaatc gaaagttcta gaaactgcct 601 tgaaagatcc agaacaaaaa gtcctgaaaa tccaaaacga tagtgtagct attgaaactc 661 aagcgattac agacaattgc tctgaagaaa gaaagacatt caacttgaac gtccaaatga 721 actcaatgga catccgttgc agtgacatca tccagggaga cacacaaggt cccagtgcca 781 ttgcctttat ctcatattct tctcttggaa acatcataaa tgcaactttt tttgaagaga 841 tggataagaa agatcaagtg tatctgaact ctcaggttgt gagtgctgct attggaccca 901 aaaggaacgt gtctctctcc aagtctgtga cgctgacttt ccagcacgtg aagatgaccc 961 ccagtaccaa aaaggtcttc tgtgtctact ggaagagcac agggcagggc agccagtggt 1021 ccagggatgg ctgcttcctg atacacgtga acaagagtca caccatgtgt aattgcagtc 1081 acctgtccag cttcgctgtc ctgatggccc tgaccagcca ggaggaggat cccgtgctga 1141 ctgtcatcac ctacgtgggg ctgagcgtct ctctgctgtg cctcctcctg gcggccctca 1201 cttttctcct gtgtaaagcc atccagaaca ccagcacctc actgcatctg cagctctcgc 1261 tctgcctctt cctggcccac ctcctcttcc tcgtggggat tgatcgaact gaacccaagg 1321 tgctgtgctc catcatcgcc ggtgctttgc actatctcta cctggccgcc ttcacctgga 1381 tgctgctgga gggtgtgcac ctcttcctca ctgcacggaa cctgacagtg gtcaactact 1441 caagcatcaa tagactcatg aagtggatca tgttcccagt cggctatggc gttcccgctg 1501 tgactgtggc catttctgca gcctcctggc ctcaccttta tggaactgct gatcgatgct 1561 ggctccacct ggaccaggga ttcatgtgga gtttccttgg cccagtctgt gccattttct 1621 ctgcgaattt agtattgttt atcttggtct tttggatttt GAAAAGAAAA CTTTCCTCCC 1681 TCAATAGTGA AGTGTCAACC ATCCAGAACA CAAGGATGCT GGCTTTCAAA GCAACAGCTC 1741 AGCTCTTCAT CCTGGGCTGC ACATGGTGTC TGGGCTTGCT ACAGGTGGGT CCAGCTGCCC 1801 AGGTCATGGC CTACCTCTTC ACCATCATCA ACAGCCTCCA AGGCTTCTTC ATCTTCTTGG 1861 TCTACTGCCT CCTCAGCCAG CAGGTCCAGA AACAATATCA AAAGTGGTTT AGAGAGATCG 1921 TAAAATCAAA ATCTGAGTCT GAGACATACA CACTTTCCAG CAAGATGGGT CCTGACTCAA 1981 AACCCAGTGA GGGGGATGTT TTTCCAGGAC AAGTGAAGAG AAAATATGAC CCAGCTTTCT 2041 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2101 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2161 GTGGAAAGGA CGAAAAGGTG TCCGTACCCT GTTCCGAACG CGTTAAGTCg acaatcaacc 2221 tctggattac aaaatttgtg aaagatt