Construct: ORF TRCN0000491464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003125.1_s317c1
- Derived from:
- ccsbBroadEn_12370
- DNA Barcode:
- CGAGTGGTTGACCCCTTCCATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP97 (57587)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57587 | CFAP97 | cilia and flagella associat... | NM_001292033.2 | 99.8% | 99.7% | 240C>T;712A>G |
| 2 | human | 57587 | CFAP97 | cilia and flagella associat... | NM_020827.3 | 83.4% | 82.4% | (many diffs) |
| 3 | human | 57587 | CFAP97 | cilia and flagella associat... | XM_017008483.2 | 83.4% | 82.4% | (many diffs) |
| 4 | human | 57587 | CFAP97 | cilia and flagella associat... | XM_017008484.2 | 83.4% | 82.4% | (many diffs) |
| 5 | human | 57587 | CFAP97 | cilia and flagella associat... | XM_017008486.2 | 83.4% | 82.4% | (many diffs) |
| 6 | human | 57587 | CFAP97 | cilia and flagella associat... | XM_024454160.1 | 83.4% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcagtttgga gatatattag aaggtgaagt ggaccattct ttctttgaca 121 gtgactttga agaaggaaag aaatgtgaaa ctaactcagt ttttgacaag caaaatgatg 181 acccaaagga aagaatagat aaagatacaa aaaatgtaaa ttcgaacact ggaatgcaaa 241 caacagaaaa ttatcttact gagaagggaa atgaaagaaa cgtgaaattt cccccagaac 301 accctgtaga gaatgatgtt acacaaactg taagttcttt ctcattgcca gcctcttcaa 361 gatcaaaaaa attgtgtgat gttacaacag gacttaaaat acacgtgtcc attccaaata 421 gaattcccaa aattgtaaaa gaaggtgaag atgattacta cacagatgga gaggaaagca 481 gtgatgatgg gaagaaatac catgtgaagt ccaagtccgc taaaccatct actaacgtta 541 aaaaaagcat aaggaaaaag tattgcaaag ttagctcctc ttcctcctcc tctttatctt 601 cctcatcttc aggttcaggt acagattgtt tagatgcagg gtctgatagc catctatctg 661 attcgtctcc gtcatctaag tcatctaaga aacatgtatc tggtataacc ctcctgtcac 721 caaaacacaa gtataaatca ggaataaaat cgacagaaac acagccttca agtactgcac 781 caaaatgtgg ccactaccct gaggagtctg aagatactgt gactgacgta agtcccttat 841 caactccaga cattagccct cttcagtctt ttgaactggg catagcaaat gatcaaaaag 901 tgaaaattaa aaagcaagaa aatgtgagcc aagaaatata tgaagatgtt gaggatttga 961 aaaataattc aaaatatttg aaagcagcca aaaaagggaa agaaaaacat gagcctgatg 1021 tctccTCAAA GTCGTCTTCA GTGTTAGACT CCAGTTTAGA CCACAGACAT AAACAGAAAG 1081 TCTTACATGA CACAATGGAT CTGAATCATC TCTTGAAAGC TTTTCTGCAA TTAGATAAAA 1141 AAGGACCACA AAAACATCAC TTTGATCAGC CTTCAGTAGC ACCCGGGAAA AACTACTCTT 1201 TCACAAGAGA AGAGGTGAGA CAGATCGATC GGGAAAATCA GAGGCTTTTG AAAGAACTGT 1261 CAAGACAGGC GGAAAAGCCG GGAAGCAAAA GTACAATTCC TAGATCGGCT GATCATCCCC 1321 CAAAGTTATA TCACAGTGCT CTCAACAGAC AGAAGGAACA ACAAAGGATT GAGAGAGAAA 1381 ACTTGACTAT CTCGCTCTGT CACCCAGGCT GGAGTGCAGT GACGTGCCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA CGAGTGGTTG ACCCCTTCCA TATAACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt