Construct: ORF TRCN0000491466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005985.1_s317c1
- Derived from:
- ccsbBroadEn_10716
- DNA Barcode:
- TTAGGCATCGGGTTCTGATAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD22 (933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491466
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 933 | CD22 | CD22 molecule | NM_001278417.2 | 100% | 100% | |
| 2 | human | 933 | CD22 | CD22 molecule | NM_001771.4 | 79.6% | 79.3% | (many diffs) |
| 3 | human | 933 | CD22 | CD22 molecule | NM_001185099.2 | 69.2% | 68.9% | (many diffs) |
| 4 | human | 933 | CD22 | CD22 molecule | NM_001185100.2 | 68.2% | 66.3% | (many diffs) |
| 5 | human | 933 | CD22 | CD22 molecule | NM_001185101.1 | 58.7% | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2094
- ORF length:
- 2025
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctctgtgg ctcctagagg gggttccaat gaggcaggct gctgtcacct 121 cgacctcctt gaccatcaag tctgtcttca cccggagcga gctcaagttc tccccacagt 181 ggagtcacca tgggaagatt gtgacctgcc agcttcagga tgcagatggg aagttcctct 241 ccaatgacac ggtgcagctg aacgtgaagc acaccccgaa gttggagatc aaggtcactc 301 ccagtgatgc catagtgagg gagggggact ctgtgaccat gacctgcgag gtcagcagca 361 gcaacccgga gtacacgacg gtatcctggc tcaaggatgg gacctcgctg aagaagcaga 421 atacattcac gctaaacctg cgcgaagtga ccaaggacca gagtgggaag tactgctgtc 481 aggtctccaa tgacgtgggc ccgggaaggt cggaagaagt gttcctgcaa gtgcagtatg 541 ccccggaacc ttccacggtt cagatcctcc actcaccggc tgtggaggga agtcaagtcg 601 agtttctttg catgtcactg gccaatcctc ttccaacaaa ttacacgtgg taccacaatg 661 ggaaagaaat gcagggaagg acagaggaga aagtccacat cccaaagatc ctcccctggc 721 acgctgggac ttattcctgt gtggcagaaa acattcttgg tactggacag aggggcccgg 781 gagctgagct ggatgtccag tatcctccca agaaggtgac cacagtgatt caaaacccca 841 tgccgattcg agaaggagac acagtgaccc tttcctgtaa ctacaattcc agtaacccca 901 gtgttacccg gtatgaatgg aaaccccatg gcgcctggga ggagccatcg cttggggtgc 961 tgaagatcca aaacgttggc tgggacaaca caaccatcgc ctgcgcagct tgtaatagtt 1021 ggtgctcgtg ggcctcccct gtcgccctga atgtccagta tgccccccga gacgtgaggg 1081 tccggaaaat caagcccctt tccgagattc actctggaaa ctcggtcagc ctccaatgtg 1141 acttctcaag cagccacccc aaagaagtcc agttcttctg ggagaaaaat ggcaggcttc 1201 tggggaaaga aagccagctg aattttgact ccatctcccc agaagatgct gggagttaca 1261 gctgctgggt gaacaactcc ataggacaga cagcgtccaa ggcctggaca cttgaagtgc 1321 tgtatgcacc caggaggctg cgtgtgtcca tgagcccggg ggaccaagtg atggagggga 1381 agagtgcaac cctgacctgt gagagcgacg ccaaccctcc cgtctcccac tacacctggt 1441 ttgactggaa taaccaaagc ctcccctacc acagccagaa gctgagattg gagccggtga 1501 aggtccagca ctcgggtgcc tactggtgcc aggggaccaa cagtgtgggc aagggccgtt 1561 cgcctctcag caccctcacc gtctactata gcccggagac catcggcagg cgagtggctg 1621 tgggactcgg gtcctgcctc gccatcctca tcctggcaat ctgtgggctc aagctccagc 1681 gacgttggaa gaggacacag agccagcagg ggcttcagga gaattccagc ggccagagct 1741 tctttgtgag gaataaaaag gttagaaggg cccccctctc tgaaggcccc cactccctgg 1801 gatgctacaa tccaatgatg gaagatggca ttagctacac cacccTGCGC TTTCCCGAGA 1861 TGAACATACC ACGAACTGGA GATGCAGAGT CCTCAGAGAT GCAGAGACCT CCCCCGGACT 1921 GCGATGACAC GGTCACTTAT TCAGCATTGC ACAAGCGCCA AGTGGGCGAC TATGAGAACG 1981 TCATTCCAGA TTTTCCAGAA GATGAGGGGA TTCATTACTC AGAGCTGATC CAGTTTGGGG 2041 TCGGGGAGCG GCCTCAGGCA CAAGAAAATG TGGACTATGT GATCCTCAAA CATTTGCCAA 2101 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2161 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2221 TATCTTGTGG AAAGGACGAT TAGGCATCGG GTTCTGATAA CTAACGCGTT AAGTCgacaa 2281 tcaacctctg gattacaaaa tttgtgaaag att