Construct: ORF TRCN0000491474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010028.1_s317c1
- Derived from:
- ccsbBroadEn_00524
- DNA Barcode:
- ATTACAGCCGCGCACCCACGCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXT2 (2132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2132 | EXT2 | exostosin glycosyltransfera... | NM_207122.1 | 100% | 100% | |
| 2 | human | 2132 | EXT2 | exostosin glycosyltransfera... | XM_011519951.1 | 98.2% | 98.2% | 1_39del |
| 3 | human | 2132 | EXT2 | exostosin glycosyltransfera... | NM_001178083.2 | 97.3% | 96.2% | (many diffs) |
| 4 | human | 2132 | EXT2 | exostosin glycosyltransfera... | NM_000401.3 | 95.6% | 95.6% | 1_99del |
| 5 | human | 2132 | EXT2 | exostosin glycosyltransfera... | XM_011519950.1 | 93.9% | 93.9% | 1_138del |
| 6 | human | 2132 | EXT2 | exostosin glycosyltransfera... | XM_024448383.1 | 57.9% | 57.3% | (many diffs) |
| 7 | mouse | 14043 | Ext2 | exostoses (multiple) 2 | NM_010163.3 | 87.8% | 95.4% | (many diffs) |
| 8 | mouse | 14043 | Ext2 | exostoses (multiple) 2 | XM_006498733.3 | 87.8% | 95.4% | (many diffs) |
| 9 | mouse | 14043 | Ext2 | exostoses (multiple) 2 | XM_006498732.3 | 86% | 93.4% | (many diffs) |
| 10 | mouse | 14043 | Ext2 | exostoses (multiple) 2 | XM_006498731.3 | 84.3% | 91.5% | (many diffs) |
| 11 | mouse | 14043 | Ext2 | exostoses (multiple) 2 | XM_006498730.3 | 81.6% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2220
- ORF length:
- 2154
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgcgtcggtc aagtataata tccggggtcc tgccctcatc ccaagaatga 121 agaccaagca ccgaatctac tatatcaccc tcttctccat tgtcctcctg ggcctcattg 181 ccactggcat gtttcagttt tggccccatt ctatcgagtc ctcaaatgac tggaatgtag 241 agaagcgcag catccgtgat gtgccggttg ttaggctgcc agccgacagt cccatcccag 301 agcgggggga tctcagttgc agaatgcaca cgtgttttga tgtctatcgc tgtggcttca 361 acccaaagaa caaaatcaag gtgtatatct atgctctgaa aaagtacgtg gatgactttg 421 gcgtctctgt cagcaacacc atctcccggg agtataatga actgctcatg gccatctcag 481 acagtgacta ctacactgat gacatcaacc gggcctgtct gtttgttccc tccatcgatg 541 tgcttaacca gaacacactg cgcatcaagg agacagcaca agcgatggcc cagctctcta 601 ggtgggatcg aggtacgaat cacctgttgt tcaacatgtt gcctggaggt cccccagatt 661 ataacacagc cctggatgtc cccagagaca gggccctgtt ggctggtggc ggcttttcta 721 cgtggactta ccggcaaggc tacgatgtca gcattcctgt ctatagtcca ctgtcagctg 781 aggtggatct tccagagaaa ggaccaggtc cacggcaata cttcctcctg tcatctcagg 841 tgggtctcca tcctgagtac agagaggacc tagaagccct ccaggtcaaa catggagagt 901 cagtgttagt actcgataaa tgcaccaacc tctcagaggg tgtcctttct gtccgtaagc 961 gctgccacaa gcaccaggtc ttcgattacc cacaggtgct acaggaggct actttctgtg 1021 tggttcttcg tggagctcgg ctgggccagg cagtattgag cgatgtgtta caagctggct 1081 gtgtcccggt tgtcattgca gactcctata ttttgccttt ctctgaagtt cttgactgga 1141 agagagcatc tgtggttgta ccagaagaaa agatgtcaga tgtgtacagt attttgcaga 1201 gcatccccca aagacagatt gaagaaatgc agagacaggc ccggtggttc tgggaagcgt 1261 acttccagtc aattaaagcc attgccctgg ccaccctgca gattatcaat gaccggatct 1321 atccatatgc tgccatctcc tatgaagaat ggaatgaccc tcctgctgtg aagtggggca 1381 gcgtgagcaa tccactcttc ctcccgctga tcccaccaca gtctcaaggg ttcaccgcca 1441 tagtcctcac ctacgaccga gtagagagcc tcttccgggt catcactgaa gtgtccaagg 1501 tgcccagtct atccaaacta cttgtcgtct ggaataatca gaataaaaac cctccagaag 1561 attctctctg gcccaaaatc cgggttccat taaaagttgt gaggactgct gaaaacaagt 1621 taagtaaccg tttcttccct tatgatgaaa tcgagacaga agctgttctg gccattgatg 1681 atgatatcat tatgctgacc tctgacgagc tgcaatttgg ttatgaggtc tggcgggaat 1741 ttcctgaccg gttggtgggt tacccgggtc gtctgcatct ctgggaccat gagatgaata 1801 agtggaagta tgagtctgag tggacgaatg aagtgtccat ggtgctcact ggggcagctt 1861 tttatcacaa gtattttaat tacctgtata cctacaaaat gcctggGGAT ATCAAGAACT 1921 GGGTAGATGC TCATATGAAC TGTGAAGATA TTGCCATGAA CTTCCTGGTG GCCAACGTCA 1981 CGGGAAAAGC AGTTATCAAG GTAACCCCAC GAAAGAAATT CAAGTGTCCT GAGTGCACAG 2041 CCATAGATGG GCTTTCACTA GACCAAACAC ACATGGTGGA GAGGTCAGAG TGCATCAACA 2101 AGTTTGCTTC AGTCTTCGGG ACCATGCCTC TCAAGGTGGT GGAACACCGA GCTGACCCTG 2161 TCCTGTACAA AGATGACTTT CCTGAGAAGC TGAAGAGCTT CCCCAACATT GGCAGCTTAT 2221 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2281 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2341 TTTATATATC TTGTGGAAAG GACGAATTAC AGCCGCGCAC CCACGCCATA CGCGTTAAGT 2401 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt