Construct: ORF TRCN0000491479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021693.2_s317c1
- DNA Barcode:
- CGCCAGACTGTCTGACGGCAGATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FGFR4 (2264)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_022963.3 | 51.6% | 1.8% | (many diffs) |
| 2 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_001291980.2 | 50.5% | 1.6% | (many diffs) |
| 3 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_001354984.2 | 49.9% | 1.7% | 1_1204del;1740T>C |
| 4 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_002011.5 | 49.9% | 1.7% | 1_1204del;1740T>C |
| 5 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_213647.3 | 49.9% | 1.7% | 1_1204del;1740T>C |
| 6 | mouse | 14186 | Fgfr4 | fibroblast growth factor re... | XM_006517100.1 | 63% | 2.5% | (many diffs) |
| 7 | mouse | 14186 | Fgfr4 | fibroblast growth factor re... | XM_006517099.2 | 50% | 1.7% | (many diffs) |
| 8 | mouse | 14186 | Fgfr4 | fibroblast growth factor re... | NM_008011.2 | 43.6% | 1.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 239
- ORF end:
- 344
- ORF length:
- 105
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctcgcc cgccactgtg cagaagctct cccgcttccc tctggcccga cagttctccc 121 tggagtcagg ctcttccggc aagtcaagct catccctggt acgaggcgtg cgtctctcct 181 ccagcggccc cgccttgctc gccggcctcg tgagtctaga tctacctctc gacccactat 241 gggagttccc ccgggacagg ctggtgcttg ggaagcccct aggcgagggc tgctttggcc 301 aggtagtacg tgcagaggcc tttggcatgg accctgcccg gcctgaccaa gccagcactg 361 tggccgtcaa gatgctcaaa gacaacgcct ctgacaagga cctggccgac ctggtctcgg 421 agatggaggt gatgaagctg atcggccgac acaagaacat catcaacctg cttggtgtct 481 gcacccagga agggcccctg tacgtgatcg tggagtgcgc cgccaaggga aacctgcggg 541 agttcctgcg ggcccggcgc cccccaggcc ccgacctcag ccccgacggt cctcggagca 601 gcgaggggcc gctctccttc ccagtcctgg tctcctgcgc ctaccaggtg gcccgaggca 661 tgcagtatct ggagtcccgg aagtgtatcc accgggacct ggctgcccgc aatgtgctgg 721 tgactgagga caatgtgatg aagattgctg actttgggct ggcccgcggc gtccaccaca 781 ttgactacta taagaaaacc agcaacggcc gcctgcctgt gaagtggatg gcgcccgagg 841 ccttgtttga ccgggtgtac acacaccaga gtgacgtgtg gtcttttggg atcctgctat 901 gggagatctt caccctcggg ggctccccgt atcctggcat cccggtggag gagctgttct 961 cgctgctgcg ggagggacat cggatggaCC GACCCCCACA CTGCCCCCCA GAGCTGTACG 1021 GGCTGATGCG TGAGTGCTGG CACGCAGCGC CCTCCCAGAG GCCTACCTTC AAGCAGCTGG 1081 TGGAGGCGCT GGACAAGGTC CTGCTGGCCG TCTCTGAGGA GTACCTCGAC CTCCGCCTGA 1141 CCTTCGGACC CTATTCCCCC TCTGGTGGGG ACGCCAGCAG CACCTGCTCC TCCAGCGATT 1201 CTGTCTTCAG CCACGACCCC CTGCCATTGG GATCCAGCTC CTTCCCCTTC GGGTCTGGGG 1261 TGCAGACATG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGC CAGACTGTCT GACGGCAGAT 1441 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t