Construct: ORF TRCN0000491480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021519.2_s317c1
- DNA Barcode:
- GAATACGCGCGTGTCACATCCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KIF3C (3797)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3797 | KIF3C | kinesin family member 3C | XM_005264299.3 | 95% | 94.9% | 1890_2006del |
| 2 | human | 3797 | KIF3C | kinesin family member 3C | NM_002254.8 | 94.9% | 94.8% | 1890_2009del |
| 3 | mouse | 16570 | Kif3c | kinesin family member 3C | NM_008445.2 | 84.6% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2328
- ORF length:
- 2259
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccagtaag accaaggcca gcgaggccct caaggtggtg gcccggtgcc 121 gccccctcag caggaaggag gaggctgctg gtcacgagca gatcctgacc atggacgtga 181 aactgggcca ggtgaccctg cggaaccccc gcgccgcccc gggggagctg cccaagacct 241 tcacctttga cgccgtgtat gatgccagct ccaagcaggc cgacctgtat gacgaaaccg 301 tgaggcccct gatagactcc gtgctccagg gtttcaatgg cacggtgttt gcctatggcc 361 agacgggcac tggcaagacc tataccatgc aggggacctg ggtggagccc gagctgcgcg 421 gggtcatccc gaatgccttt gagcacatct tcacccacat ctcccgctcc cagaaccaac 481 agtacctggt ccgggcctcc tatttggaga tctaccagga agagattcga gacctgctct 541 ccaaggagcc gggcaagagg ctagagctga aagagaaccc cgagactggc gtctacatca 601 aggacctctc ctccttcgtc accaagaatg tcaaggagat tgagcatgtg atgaacctgg 661 ggaaccagac ccgggctgtg ggcagcaccc acatgaatga ggtcagctcc cgctcccatg 721 ccatcttcat catcactgtg gagtgcagcg aacgtggctc tgatggccag gaccacatcc 781 gagtgggcaa gctcaacctc gtggacctgg ctggcagcga gaggcagaac aaggcaggcc 841 ccaacacagc gggaggggca gccacaccat cctcgggtgg cggtggtggc ggtggaggca 901 gtggtggtgg tgctggtgga gagaggccta aggaagcctc caaaatcaac ctctcattat 961 ctgccctggg caacgtgatt gctgccctgg cgggcaacag gagcacccac attccctacc 1021 gggactccaa gctgacccgg ctgctccagg actccctggg ggggaatgcc aagaccatca 1081 tggtagccac actggggcca gcttctcaca gctacgatga gagcctctcc accttgcgct 1141 ttgccaaccg agccaagaac atcaagaaca agccccgggt gaacgaggac cccaaggaca 1201 cactgctgcg ggaattccaa gaggagattg cccgcctgaa ggcccagctg gagaagaggg 1261 ggatgctggg gaagcggccc cggaggaaga gcagccgcag gaagaaggcc gtgtccgccc 1321 cgcctgggta ccctgagggc ccagtgattg aggcctgggt ggcagaagag gaggatgaca 1381 acaacaacaa ccaccgcccg ccccagccca tcctggagtc agccttggag aagaacatgg 1441 agaattacct gcaggaacag aaggagcggc tggaggagga gaaggcagcc atccaggatg 1501 accgcagcct ggtgagcgag gagaagcaga agctgctgga ggagaaggag aagatgctgg 1561 aggacctgcg gcgggaacag caggccacag agctgcttgc ggccaagtac aaggccatgg 1621 agagcaagct cctcatcggg ggcaggaaca tcatggatca caccaacgaa cagcagaaga 1681 tgttggaact gaagaggcag gagattgccg agcagaaacg tcgtgagcgg gagatgcagc 1741 aggagatgat gctccgggac gaggagacta tggagctccg gggcacctac acatccctgc 1801 agcaggaggt ggaggtcaaa accaagaaac tcaagaagct ctacgccaag ctgcaggcgg 1861 tgaaggcgga gatccaggac cagcatgatg agtatatccg cgtgcggcag gacctggagg 1921 aggcgcagaa cgagcagacc cgcgaactca agctcaatag cagccagatg aagaagcggc 1981 caacatctgc agtgggctac aagaggccta tcagccagta tgctcgggtt gccatGGCAA 2041 TGGGGTCCCA CCCCAGGTAC AGGGCTGAAA ACATAATGTT TCTGGAGTTG GATGTGTCCC 2101 CTCCAGCTGT CTTTGAGATG GAATTCTCTC ACGACCAAGA ACAAGACCCT CGTGCGCTAC 2161 ACATGGAGAG GCTCATGCGA TTGGACAGCT TTCTGGAAAG ACCTTCCACG TCTAAAGTCC 2221 GAAAGTCCAG ATCCTGGTGC CAGAGTCCTC AGCGGCCTCC ACCTTCCACC ACACATGCCT 2281 CCCTGGCCTC TGCTTCTCTG CGCCCTGCAA CAGTGGCGGA CCATGAGTGA GACCCAGCTT 2341 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2401 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2461 CTTGTGGAAA GGACGAGAAT ACGCGCGTGT CACATCCGTC ACGCGTTAAG TCgacaatca 2521 acctctggat tacaaaattt gtgaaagatt