Construct: ORF TRCN0000491489
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013515.1_s317c1
- Derived from:
- ccsbBroadEn_01623
- DNA Barcode:
- ACTTACCTAAAGCCTCTCCGCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUPT4H1 (6827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491489
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6827 | SUPT4H1 | SPT4 homolog, DSIF elongati... | NM_003168.3 | 100% | 100% | |
| 2 | human | 6827 | SUPT4H1 | SPT4 homolog, DSIF elongati... | NR_073470.2 | 23.5% | 1_66del;136_137delGT;420_1490del | |
| 3 | mouse | 100041294 | Supt4b | suppressor of Ty 4B | NM_011509.2 | 95.1% | 99.1% | (many diffs) |
| 4 | mouse | 20922 | Supt4a | suppressor of Ty 4A | NM_009296.1 | 94.8% | 100% | (many diffs) |
| 5 | mouse | 20922 | Supt4a | suppressor of Ty 4A | XM_006532741.2 | 59.3% | 50% | (many diffs) |
| 6 | mouse | 20922 | Supt4a | suppressor of Ty 4A | XM_006532742.2 | 59.3% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctggagacg gtgccgaagg accTGCGGCA TCTGCGGGCC TGTTTGCTGT 121 GTTCGCTGGT CAAGACTATA GACCAGTTTG AATATGATGG TTGTGACAAT TGTGATGCAT 181 ATCTACAAAT GAAGGGTAAC CGAGAGATGG TATATGACTG CACTAGCTCT TCCTTTGATG 241 GAATCATTGC GATGATGAGT CCAGAGGACA GCTGGGTCTC CAAGTGGCAG CGAGTCAGTA 301 ACTTTAAGCC AGGTGTATAT GCGGTGTCAG TCACTGGTCG CCTGCCCCAA GGAATCGTGC 361 GGGAGCTGAA AAGTCGAGGA GTGGCCTACA AATCCAGAGA CACAGCTATA AAGACCTACC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAACTTACCT AAAGCCTCTC CGCCGCACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt