Construct: ORF TRCN0000491493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019675.2_s317c1
- DNA Barcode:
- ATTAGACTCCTCTTTCGAACACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMKK1 (84254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | NM_032294.3 | 99.9% | 99.8% | 1515_1516insG |
| 2 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_017025220.2 | 99.9% | 99.8% | 1515_1516insG |
| 3 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | NM_172206.2 | 94.8% | 94.7% | 1_81del;1596_1597insG |
| 4 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_005256817.1 | 92.9% | 92.8% | 685_798del;1629_1630insG |
| 5 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_006721588.3 | 92.9% | 92.8% | 685_798del;1629_1630insG |
| 6 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | NM_172207.2 | 88.7% | 88.6% | 685_798del;1560_1561ins70 |
| 7 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450996.1 | 88.5% | 88.4% | 1_81del;766_879del;1710_1711insG |
| 8 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524031.3 | 84.5% | 84.4% | 1_81del;766_879del;1641_1642ins70 |
| 9 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XR_001752661.2 | 81.4% | 1_312del;995_996ins22;1806_1812delTGAGCCCinsG | |
| 10 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450997.1 | 58.7% | 56.3% | (many diffs) |
| 11 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524033.1 | 52.8% | 52.7% | 0_1ins714;801_802insG |
| 12 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524035.3 | 52.8% | 52.7% | 0_1ins714;801_802insG |
| 13 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450998.1 | 52.8% | 52.7% | 0_1ins714;801_802insG |
| 14 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450999.1 | 52.8% | 52.7% | 0_1ins714;801_802insG |
| 15 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524032.3 | 48.4% | 41.3% | (many diffs) |
| 16 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XR_001752662.2 | 33.3% | (many diffs) | |
| 17 | mouse | 55984 | Camkk1 | calcium/calmodulin-dependen... | NM_018883.2 | 88.6% | 93.2% | (many diffs) |
| 18 | mouse | 55984 | Camkk1 | calcium/calmodulin-dependen... | XM_006533760.3 | 88.6% | 93.2% | (many diffs) |
| 19 | mouse | 55984 | Camkk1 | calcium/calmodulin-dependen... | XM_006533761.3 | 88.6% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1593
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag gggggtccag ctgtctgctg ccaggatcct cgggcagagc 121 tggtagaacg ggtggcagcc atcgatgtga ctcacttgga ggaggcagat ggtggcccag 181 agcctactag aaacggtgtg gaccccccac cacgggccag agctgcctct gtgatccctg 241 gcagtacttc aagactgctc ccagcccggc ctagcctctc agccaggaag ctttccctac 301 aggagcggcc agcaggaagc tatctggagg cgcaggctgg gccttatgcc acggggcctg 361 ccagccacat ctccccccgg gcctggcgga ggcccaccat cgagtcccac cacgtggcca 421 tctcagatgc agaggactgc gtgcagctga accagtacaa gctgcagagt gagattggca 481 agggtgccta cggtgtggtg aggctggcct acaacgaaag tgaagacaga cactatgcaa 541 tgaaagtcct ttccaaaaag aagttactga agcagtatgg ctttccacgt cgccctcccc 601 cgagagggtc ccaggctgcc cagggaggac cagccaagca gctgctgccc ctggagcggg 661 tgtaccagga gattgccatc ctgaagaagc tggaccacgt gaatgtggtc aaactgatcg 721 aggtcctgga tgacccagct gaggacaacc tctatttggt gtttgacctc ctgagaaagg 781 ggcccgtcat ggaagtgccc tgtgacaagc ccttctcgga ggagcaagct cgcctctacc 841 tgcgggacgt catcctgggc ctcgagtact tgcactgcca gaagatcgtc cacagggaca 901 tcaagccatc caacctgctc ctgggggatg atgggcacgt gaagatcgcc gactttggcg 961 tcagcaacca gtttgagggg aacgacgctc agctgtccag cacggcggga accccagcat 1021 tcatggcccc cgaggccatt tctgattccg gccagagctt cagtgggaag gccttggatg 1081 tatgggccac tggcgtcacg ttgtactgct ttgtctatgg gaagtgccca ttcatcgacg 1141 atttcatcct ggccctccac aggaagatca agaatgagcc cgtggtgttt cctgaggagc 1201 cagaaatcag cgaggagctc aaggacctga tcctgaagat gttagacaag aatcccgaga 1261 cgagaattgg ggtgccagac atcaagttgc acccttgggt gaccaagaac ggggaggagc 1321 cccttccttc ggaggaggag cactgcagcg tggtggaggt gacagaggag gaggttaaga 1381 actcagtcag gcTCATCCCC AGCTGGACCA CGGTGATCCT GGTGAAGTCC ATGCTGAGGA 1441 AGCGTTCCTT TGGGAACCCG TTTGAGCCCC AAGCACGGAG GGAAGAGCGA TCCATGTCTG 1501 CTCCAGGAAA CCTACTGGTG AAAGAAGGGT TTGGTGAAGG GGGCAAGAGC CCAGAGCTCC 1561 CCGGCGTCCA GGAAGACGAG GCTGCATCCG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTAG 1741 ACTCCTCTTT CGAACACGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt